A student attempted the quantitative analysis of skim milk proteins as described in practical manual. However, they initially forgot to dilute the skim milk sample before determination of protein concentrations in the Bradford assay. The spectrophotometer was blanked, absorbance 595nm was measured in duplicate and the average protein concentration was determined. After completing the measurements they realised the error and decided to repeat the experiment and to include the dilution step. However, when doing so they erroneously applied a 40,000 X dilution instead of the required 400 X dilution and obtaind and average absorbance value of 0.002. Explain whether and how the quantitative determination of the protein concentration will be impacted by i) not diluting the sample; and ii) by applying the 40,000 X dilution factor. Justify your answer.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Chemistry
A student attempted the quantitative analysis of skim milk proteins as described in practical manual. However, they initially forgot to dilute the skim milk sample before determination of protein concentrations in the Bradford assay. The spectrophotometer was blanked, absorbance 595nm was measured in duplicate and the average protein concentration was determined. After completing the measurements they realised the error and decided to repeat the experiment and to include the dilution step. However, when doing so they erroneously applied a 40,000 X dilution instead of the required 400 X dilution and obtaind and average absorbance value of 0.002. Explain whether and how the quantitative determination of the protein concentration will be impacted by i) not diluting the sample; and ii) by applying the 40,000 X dilution factor. Justify your answer. Answer must be less than 150 words total.
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