A peptide has the sequence Glu-His-Trp-Ser-Gly-Leu-Arg-Pro-Gly The pK₂ values for the peptide's side chains, terminal amino groups, and carboxyl groups are provided in the table. Amino acid Amino pK₁ Carboxyl pKa Side-chain pKa glutamate 9.60 2.34 4.25 histidine 9.17 1.82 6.00 9.39 2.38 9.15 2.21 9.60 2.34 9.60 2.36 9.04 2.17 10.96 1.99 tryptophan serine glycine leucine arginine proline Calculate the net charge of the molecule at pH 3. Calculate the net charge of the molecule at pH 8. 12.48 net charge at pH 3: net charge at pH 8:

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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A peptide has the sequence
Glu-His-Trp-Ser-Gly-Leu-Arg-Pro-Gly
The pK₁ values for the peptide's side chains, terminal amino groups, and carboxyl groups are provided in the table.
Amino acid Amino pKa Carboxyl pKa Side-chain pKa
glutamate
9.60
2.34
4.25
histidine
9.17
1.82
6.00
9.39
2.38
9.15
2.21
9.60
2.34
9.60
2.36
9.04
2.17
10.96
1.99
tryptophan
serine
glycine
leucine
arginine
proline
Calculate the net charge of the molecule at pH 3.
Calculate the net charge of the molecule at pH 8.
12.48
net charge at pH 3:
net charge at pH 8:
Transcribed Image Text:A peptide has the sequence Glu-His-Trp-Ser-Gly-Leu-Arg-Pro-Gly The pK₁ values for the peptide's side chains, terminal amino groups, and carboxyl groups are provided in the table. Amino acid Amino pKa Carboxyl pKa Side-chain pKa glutamate 9.60 2.34 4.25 histidine 9.17 1.82 6.00 9.39 2.38 9.15 2.21 9.60 2.34 9.60 2.36 9.04 2.17 10.96 1.99 tryptophan serine glycine leucine arginine proline Calculate the net charge of the molecule at pH 3. Calculate the net charge of the molecule at pH 8. 12.48 net charge at pH 3: net charge at pH 8:
Calculate the net charge of the molecule at pH 11.
Estimate the isoelectric point (pl) for this peptide.
net charge at pH 11:
pl:
Transcribed Image Text:Calculate the net charge of the molecule at pH 11. Estimate the isoelectric point (pl) for this peptide. net charge at pH 11: pl:
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