A mixture of proteins with the following properties were purified using cation exchange chromatography. Which of the following peaks correspond to the correct protein? Protein M- pI- 11, size- 60 Kda Protein N- pI- 8, size- 112 Kda Protein O- pI- 6, size- 280 Kda Protein P- pI- 4, size- 36 Kda [N.B: I was trying to upload pictures, but it was interrupting, Peak C is the highest peak, Peak D is lowest, Peak B is higher than D; however lower than C and A, Peak A is lower than C only, higher than B and D] a) Peak A is P b) Peak C in N c) Peak D is P d) Peak B is O e) Peak A is M
1. A mixture of proteins with the following properties were purified using cation exchange chromatography. Which of the following peaks correspond to the correct protein?
Protein M- pI- 11, size- 60 Kda
Protein N- pI- 8, size- 112 Kda
Protein O- pI- 6, size- 280 Kda
Protein P- pI- 4, size- 36 Kda
[N.B: I was trying to upload pictures, but it was interrupting, Peak C is the highest peak, Peak D is lowest, Peak B is higher than D; however lower than C and A, Peak A is lower than C only, higher than B and D]
a) Peak A is P b) Peak C in N c) Peak D is P d) Peak B is O
e) Peak A is M
2. Which transition state analog is the most effective competitor for serine protease such as Chymotrypsin?
a) R-C=O-NH-R’
b) R-C=O(CH3)-NH-R’
c) R-CH(OH)-CH(NH2)-R’
d) R-CH(OH)-NH-R’
e) R-CH=NR’
3. If a 14C fatty acid labelled with C14 is fed to a cow, after 30 minutes of Beta oxidation, where will the C14 be found?
a) Acetyl coA
b) Propionyl coA
c) Glutamine
d) Glucose
4. How many carbons will be removed from 16C fatty acids during beta oxidation?
5. What are the inhibitors of the enzyme phosphofructokinase-1 (PFK-1)?
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