A missense mutation in one of the enzymes encoded by the trp operon prevents a bacterial cell from producing tryptophan. What is the effect (if any) of this mutation on transcription of the trp operon? The trp operon will be transcribed whether or not tryptophan is supplied in the medium. The trp operon will not be transcribed under any conditions. The trp operon will be transcribed only if tryptophan is supplied in the medium. O The mutation will have no effect on transcription of the trp operon. The trp operon will be transcribed, unless tryptophan is supplied in the medium.

A missense mutation in one of the enzymes encoded by the trp operon prevents a bacterial cell from producing tryptophan. What is the effect (if any) of this mutation on transcription of the trp operon? The trp operon will be transcribed whether or not tryptophan is supplied in the medium. The trp operon will not be transcribed under any conditions. The trp operon will be transcribed only if tryptophan is supplied in the medium. O The mutation will have no effect on transcription of the trp operon. The trp operon will be transcribed, unless tryptophan is supplied in the medium.

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 11TYU

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Which of the following statements about the trp operon is false? If a mutation in region 2 prevents the formation of the 2 + 3 loop in the 5’UTR transcription levels will increase. The trp operon is an example of negative repressible operon. Formation of the anti-terminator in the 5’UTR of the trp operon occurs when trp levels are low. In the presence of high trp-tRNA, the genes of the trp operon are not expressed. The role of trp is to bind to the repressor protein and activate it.
What would happen if the operator sequence of the trp operon contained a mutation that prevented the repressor protein from binding to the operator? (Explain what would happen in both the presence and absence of tryptophan)
How will transcription of the E. coli trp operon be affected by the following manipulations of the leader region of the trp mRNA? - Changing several nucleotides in region 3 so that in can base pair with region 4 but not with region 2 - Removing region 4 - Increasing the distance between region 2 and region 3 - Increasing the distance (number of bases) between the leader peptide gene and region 2
Which of the following statements about the lactose operon in E. coli is TRUE? It contains the genes that encode the proteins for lactose metabolism. Its transcription is blocked when allolactose binds to the lac repressor. It contains a promoter, an operator and an attenuator. It is barely transcribed, when lactose is present in the medium and glucose is absent. It is transcribed at very high levels, when lactose is absent, and glucose is present.
The trp operon is a repressible operon. A strain of e coli develops a mutation whereby the trp repressor protein can no longer bind to the operator. How will this effect the production of tryptophan producing enzymes? Will these bacteria be able to live in a tryptophan free media (bacteria food)?
Which of the following conditions is most likely to result in an increase rate of transcription of the lac operon? when lactose is present AND the repressor is permanently attached to the operator when lactose is present and there is high levels of glucose when lactose is absent and there are low levels of glucose when lactose is present and there are low levels of glucose
As diagrammed below, some amino acid biosynthetic operons have stem loop structures in the mRNA. These structures play a role in the regulation of the operon by which of the following mechanisms? Translation stop codon- Attenuator loop. mRNA 5' 3' DNA attenuator trpE O Catabolite repressor protein can bind and prevent the hairpins from forming thus allowing translation to occur. O The leader sequence shown contains codons for the amino acid. When these are low, the ribosome stalls, allowing a hairpin between the two middle regions to form so that the rest of the message can be translated. In the presence of large amounts of the amino acid, the hairpin will wind around the amino acid and prevent further translation. O A repressor protein made of that amino acid will bind to the region and prevent translation when large amounts of the amino acid are present. O The uridine-rich region has codons for that amino acid; when present, this region will be a terminator region. The hairpins allow…
In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is removed from its co-repressor, causing it to detach from the operator tryptophan is removed from its co-repressor, causing it to attach to the operator tryptophan is bound to its co-repressor, causing it to detach from the operator tryptophan is bound to its co-repressor, causing it to attach to the operator all of the above occur at equal rates when tryptophan levels get too high (Option C is the answer for the question above) What effect will the above process have on the tryptophan operon? RNA polymerase will detach from the promoter, initiating transcription RNA polymerase will detach from the promoter, stopping transcription RNA polymerase will attach to the promoter, initiating transcription RNA polymerase will attach to the promoter, stopping transcription all of the above occur at equal rates when tryptophan levels get too high
What would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)
In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is removed from its co-repressor, causing it to detach from the operator tryptophan is removed from its co-repressor, causing it to attach to the operator tryptophan is bound to its co-repressor, causing it to detach from the operator tryptophan is bound to its co-repressor, causing it to attach to the operator all of the above occur at equal rates when tryptophan levels get too high (Option D is the answer for the question above) What effect will the above process have on the tryptophan operon? RNA polymerase will detach from the promoter, initiating transcription RNA polymerase will detach from the promoter, stopping transcription RNA polymerase will attach to the promoter, initiating transcription RNA polymerase will attach to the promoter, stopping transcription all of the above occur at equal rates when tryptophan levels get too high
In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is removed from its co-repressor, causing it to detach from the operator tryptophan is removed from its co-repressor, causing it to attach to the operator tryptophan is bound to its co-repressor, causing it to detach from the operator tryptophan is bound to its co-repressor, causing it to attach to the operator all of the above occur at equal rates when tryptophan levels get too high What effect will the above process have on the tryptophan operon? RNA polymerase will detach from the promoter, initiating transcription RNA polymerase will detach from the promoter, stopping transcription RNA polymerase will attach to the promoter, initiating transcription RNA polymerase will attach to the promoter, stopping transcription all of the above occur at equal rates when tryptophan levels get too high
When referring to attenuation in the regulation of the trp operon it would be safe to say that, when there are high levels of tryptophan available to the organism, transcription termination at the attenuator region is likely the ribosomes stall during translation of the attenuator region translation termination of the trp operon is likely the trp operon is transcribed at relatively high levels tryptophan inactivates the repressor protein