A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation A = 0.0182x + 0.007 where A is the corrected absorbance of the solution and x is quantity of protein in the solution in units of micrograms (µg). Determine the quantity of protein in a solution that has an absorbance of 0.344. A blank solution has an absorbance of 0.095.

A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation A = 0.0182x + 0.007 where A is the corrected absorbance of the solution and x is quantity of protein in the solution in units of micrograms (µg). Determine the quantity of protein in a solution that has an absorbance of 0.344. A blank solution has an absorbance of 0.095.

Chemistry & Chemical Reactivity

9th Edition

ISBN:9781133949640

Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Chapter4: Stoichiometry: Quantitative Information About Chemical Reactions

Section4.8: Stoichiometry Of Reactions In Aqueous Solution-titrations

Problem 2RC

See similar textbooks

Similar questions

The equation of your standard curve of absorbance versus concentration (mM) is y = 1.245x + 0.0012. You put a solution of unknown concentration into the Spec20 and get an absorbance value of 0.227, which lies within the standards used to make the standard curve. What is the concentration of the unknown solution in mM?
1.) Beer's Law can be used to find the concentration of a solution by measuring its absorbance. The law is given by ?=???A=ϵbC Where A is the absorbance, ?ϵ is the molar absorptivity, b is the pathlength of the light (the width of the sample cell), and C is the concentration of the solution. A student is analyzing a solution of a metal, and finds that the absorbance of the solution is 0.532. If ?=187 L⋅g−1⋅cm−1ϵ=187 L⋅g−1⋅cm−1 and ?=1 cmb=1 cm , what is the concentration of the metal in solution (in g/L)? * 352 * 2.84×10−32.84×10−3 * 2.84×10−22.84×10−2 * 96.8 2.) How many significant figures does the number 0.00358 have? Group of answer choices a.1 b.2 c.3 d.5 3.) A 10.00 mL solution of a certain food dye has a concentration of 1.47 g /L. What is the total mass of food dye in the solution? Report your answer with the correct number of significant figures.
Given the following sample concentrations and corresponding absorbances, construct a standard curve of absorbance vs. concentration and determine the molar absorptivity constant. Sample Concentration 15 μΜ 25 μΜ 40 μΜ Absorbance 0.19 0.32 0.49 55 μΜ 0.70
Blue Blue dye stock solution Absorbance at 630 nm Calibration curve Part 1: 0.1944 M 0.00697 y = 0.0682x A purple solution is prepared by mixing 5.00 mL of the blue dye solution with 6.75 mL of red dye solution. The calibration curve for each color is provided. Part 2 out of 3 (a) Determine the theoretical molar concentration of each dye in the mixture. [Blueltheoretical = 8.27 x 10-2 [Red] theoretical = 1.16 [Bluelexperimental [Redlexperimental " *10-1 M M x 10 (b) Using the absorbance and calibration curve for each dye, calculate the experimental molar concentration of each dye in the mixture. x 10 Red Red dye stock solution Absorbance at 500 nm Calibration curve 0.2015 M 0.00193 y = 0.0170x M M
From experimental data, a plot of absorbance (in arbitrary units, a.u.) at a wavelength of 630 nm (on the y-axis) was plotted against the concentration (in mg/L) of Cu2+ (on the x-axis) in known solutions and a linear relationship was found with an equation of y = 7.22x+0.05. If an unknown solution had an absorbance of 1.20 a.u. at a wavelength of 630 nm, what is its concentration of Cu2+?
During your experiment, you measure the absorbance of a sample to be 1.362. You have to convert thisabsorbance to methylene blue concentration (in ppm) using the absorbance measurements of two standardsolutions of known concentration:5 ppm: Absorbance 0.55510 ppm: Absorbance 1.211What is, in ppm, the concentration of your sample? Report using 3 decimal figures. Your answer must becorrect to 0.02 ppm
2. You add 10.00 ml of a 0.630 M methyl red solution to a 100.00 ml volumetric flask. You then add small volumes of sodium acetate and acetic acid solutions to adjust the pH, and then fill the 100 mL volumetric flask to the mark with deionized water. You then make a spectroscopic measurement and obtain absorbance values of 0.253 at 520 nm and 0.079 at 420 nm. What is the concentration of [HIn] in the solution. Remember to use M;V1 = M2V2 as the first step in the calculation process. Hint: see eq. 6 in the lab manual A$20, = 0.367 A520, = 0.010 A420, - 0.012 A420, = 0.275 O 4.30 x 102 O 0.430 O 4.18 x 102 O 0.418
In an analytical laboratory a chemical compound namely Paracetamol (151.163 g/mol) is determined in a sample. A sample weighing 0.0295 g was dissolved in a solvent and diluted the solution to 1 L. The solution has λ max at 243 nm (ε=2.6×104 cm-1 mol-1 L). The solution exhibits an absorbance of 0.638 in a 2 cm cell. Calculate the percentage paracetamol in the sample.
A medical lab technician creates a standard solutions of percent hemoglobin in whole blood. The technician measures the absorbance of each solution at 520 nm and creates a standard curve of the absorbance versus percent hemoglobin in whole blood and finds the linear equation below, with an R2 of 0.9985. y= 0.0523x + 0.072 A patient sample has an absorbance of 0.893. Using the standard curve data, calculate the percent hemoglobin in the patient sample.
Based on the calibration curve, what is the molar concentration for a solution with a measured absorbance of 0.383?
The same water sample from the cave was subjected to an analysis to determine its nitrite concentration. Using a spectrophotometer, the following results were obtained for standard solutions of nitrite and for the unknown. Solution Absorbance at 550 nm 2.00 µM 0.065 6.00 µM 0.205 10.00 μΜ 0.338 14.00 μΜ 0.474 18.00 μΜ 0.598 Unknown 0.402 a. Draw a calibration curve base on the data from the standards. b. Find the slope and intercept of the curve. c. Determine the concentration of nitrite in the water sample.
A student prepared four standard solutions of (NH4)2Fe(SO4)2 and measured their absorbance at 505 nm. She created a calibration plot with the best-fit line of y = 9372 x + 0.0539. She measured the absorbance of her diluted unknown sample solution as (4.69x10^-1) nm. If she prepared her diluted sample solution by diluting 10.00 mL of the original solution to a total volume of 50.00 mL with deionized water, what is the concentration of Fe2+ in her original solution? Answer in units of M. Note: Your answer is assumed to be reduced to the highest power possible.