a) A student performed a thin layer chromatographic separation experiment. In he first run, four standard substances A – azobenzene, B - benzoin, C -cinnamic acid, and D – dextrose were spotted on the plate. The line indicates the solvent front. In he second TLC run a mixture (X) of two of hese same substances was separated. Based on Rf values, what is the identity of the unknown mixture X? b) A second mixture, Y, was run at the same АвсD ime. What is wrong with the solvent system
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- a)Determine the amount of X (in gram) in order to prepare 100 mL of 50 mM stock solution of X. Show your work. (MW of X= 225) b)Determine the volume required (mL) from the stock solution X in (i) to prepare 500 mL of MS medium containing 5 mg/L X. Show your work.You receive an order for 750 mL of 27% dextrose solution. You have available D50W, 1000 mL. How many mL of D50W and sterile water are needed to complete the order? *A 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB medium to obtain a final concentration of 50µg/ml ofampicillin?
- Normal text Calibri BI UA G |EE| +2 I +3+1 CICCO4, 1 LE | ZJ VITICHI SUpstances did not test positive for any of the organic compound Distilled water did not test positive for any of the organic compounds. 5| 6 3) 4) What do all of the indicators you have used have in common? Indicators are all substances whose solutions change color due to changes in p 5) Is there a macromolecule that the tests in this lab did not test for No, there is no macromolecule that the lab did not test for. 6) Which compound is most common in foods that come from plants? Cellocuse is the single most common biochemical compound in living things. 7) If you wanted to reduce the amount of fat in your diet, what foods would you avoid? Steer away from foods high in saturated and trans fats like fried foods, fatty meats, stick margarine, snacks and items with hydrogenated oil. M 6 8) Which foods tested would your body use for a quick burst of energy? a. For energy when no carbohydrates are available? b. For building…A student performs a separation by TLC on a silica get plate that results in three spots. Ared spot is measured 23 mm away from the original sample spot, a blue spot is 42 mmaway, and a yellow spot is 49 mm away. The distance from the original spot to where thesolvent stopped is 55 mm.a) Calculate the Rf value for each component in the mixture. (Show your work and reportthe correct number of significant figures.) b) The silica gel plate is polar. Rank the three components in the mixture from lowest tohighest polarity. Explain your answer.points) You have to make 50mL of 0.05M HCI from a 10M HCI stock solution, but the onlymeasuring devices available are 100mL graduated cylinders and 10mL serological pipes. Howcould you accurately make the dilute HCI solution (clearly explain)?
- The following strip of chromatography paper has been placed in a polar solvent Which pigments the most polar?In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.Provide a chemical test/reagent that would differentiate each of the following pairs of compounds. Also, for each test, indicate the compound (write only the corresponding CAPITAL LETTER) that would give the positive result, and the indication(s) for a positive result. ОН ОН 1. and A -CEC-H and 2. B HO CH3 OH 3. and B A
- When conducting a flame test for sodium iodide, is the result positive or negative?You are asked to prepare Murashige & Skoog (MS) medium according to concentration shown the Table. Answer the following question: 1. Prepare 250 mL stock solution (200X more concentrated) of ammonium nitrate and KNO3. 2. From the prepared ammonium nitrate stock solution above, calculate the volume required to prepare 200 mL MS medium.If I am making 500ml of a solution with 0.625ml ITS, 0.0035ml Linoleic acid, 20ml Nu-serum, 0.75g BSA, and DMEM:F12 as my solvent, how best would I make this solution and what tools would be the most accurate to use?