8. Which reporter construct design would be best for moni IGFP green when, and how much a gene-of-interest is transcribed? fluorescent protein) A. Replace the promoter with the GFP sequence, leaving the CRM and gene coding region intact. B. Include the CRM and promoter but replace the gene coding region with the GFP sequence. C. Include the CRM and promoter and add the GFP sequence to the end of the gene coding region, in the same reading frame. D. Replace the CRM with the GFP sequence and leave the promoter and gene coding region intact.
8. Which reporter construct design would be best for moni IGFP green when, and how much a gene-of-interest is transcribed? fluorescent protein) A. Replace the promoter with the GFP sequence, leaving the CRM and gene coding region intact. B. Include the CRM and promoter but replace the gene coding region with the GFP sequence. C. Include the CRM and promoter and add the GFP sequence to the end of the gene coding region, in the same reading frame. D. Replace the CRM with the GFP sequence and leave the promoter and gene coding region intact.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Concept explainers
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Question
Transcribed Image Text:8. Which reporter construct design would be best for monitoring where,
(GFP green
when, and how much a gene-of-interest is transcribed?
fluorescent protein)
A. Replace the promoter with the GFP sequence, leaving the CRM
and gene coding region intact.
B. Include the CRM and promoter but replace the gene coding
region with the GFP sequence.
C. Include the CRM and promoter and add the GFP sequence to the
end of the gene coding region, in the same reading frame.
D. Replace the CRM with the GFP sequence and leave the promoter
and gene coding region intact.
9. Which feature, if mutated, would destroy the ability of an insertion
sequence element to be transposed?
A. its transposase gene
B. its reverse transcriptase gene
C. its target site duplication
D. its inverted repeats
10. What is a poly-A tail?
A. the region where the ribosome initiates translation
B. the termination signal of bacterial translation
C. the complementary base-pairing region that forms stem-loop
structures
D. a modification of the 3' end of eukaryotic transcripts
11. Why can placental cells "afford" to express ERVs, while they are
silenced in differentiating cells during early development?
A. The placenta is a transient organ, so fitness costs are
unlikely to be high
B. ERVS stimulate the baby's immune system
C. Placental cells arise from eggs, which have most of the
cytoplasm.
D. Placental cells generate more ATP, so replication extra DNA is
not a problem.
:=
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education