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- (c) Public health scientists wanted to investigate the effect of ionising radiation on the growth of Salmonella typhimurium in the presence of antibiotics. They grew bacterial colonies of Salmonella typhimurium in petri dishes labelled A, B and C. The Petri dishes were kept at 36°C for 48 hours, with scientists observing the bacteria every 24 hours. The results for this investigation are below. Petri dish A B с Treatment Was subjected to ionising radiation followed immediately by a dose of antibiotic X An equal amount of antibiotic X was added (as was added to Petri dish A) Was given neither ionising radiation nor antibiotic X After 24 hours Small spots of growth on surface No growth Growth across whole surface After 48 hours (ii) Identify the purpose of petri dish C. Large spots of growth on surface No growth Growth across whole surface (i) Explain the effect of the ionising radiation on the bacteria. (iii) Explain why scientists must continue to develop new antibiotics.8. Pigment or color (e.g.: opaque, translucid, red, yellow, rose, violet, etc..) is also another characteristic that can be used to identify your microorganism in a slant Thus, write down the name of three microorganisms (using scientific notation: Escherichia coli) that present a pigmented pattern of growth in a slant (write down the color of each pigment) and the name of the disease that they may cause. F10 F12 F6 %24 6. R. Home Enter K F G A Shift C Page Up Page Down Alt Ctrl Alt Σ20. Bacterial spores function in bacterial reproduction . a) Trueb) False
- 6)Which of the following statements is a reason why Acid-Fast Bacteria resist the Gram stain a) Because their cell wall architecture varies from both gram-negative and gram-positive bacteria. b) Because they lack the peptidoglycan layer. c) Because they have a thick peptidoglycan layer. d) Because they have a thick layer of fatty acids.review the case scenario uploaded in the photo. in your own words: a) write a hypothesis for identifying this unknown bacteria, the suspected bacteria are e coli, pseudomonas aeruginosa, and staphylocoous aureus. b) the best option to identify is gram staining, explain in your own words the steps taken to gram stain8. Pigment or color (e.g.: opaque, translucid, red, yellow, rose, violet, etc..) is also another characteristic that can be used to identify your microorganism in a slant. Thus, write down the name of three microorganisms (using scientific notation: Escherichia coli) that present a pigmented pattern of growth in a slant (write down the color of each pigment) and the name of the disease that they may cause. 200%- 927 PM 0 日 2/7/2021 21 DELL Insert Delete F11 F12 PrtScr F9 F10 F7 F8 F6 F3 F4 F5 Esc F1 F2 Backspace & # 2$ 8 4 1 2 P Home R T E Enter K Shift V B Page Down Page Up Alt Ctrl Alt THome ノ/ *00 Σ
- 21. Gram psotive bacteria contains a tick peptidoglycan layer and an outer membrane. a) Trueb) FalseAnswer the following questions: 1. What was the first antibiotic and what was its importance? 2. What does resistance mean? 3. Who is affected by resistance? 4. What if the resistance problem is not solved? 5. Describe the structure of the bacterium (its parts) 6. Can bacteria change? explain 7. Why do Bacteria communicate, what is the purpose? 8. Explain how a bacterium achieves its resistance. 9. What is the use given to antibiotics in production animals? 10. Is this use in animals good practice? 11. Once resistance occurs, what has the scientific community had to do? 12. Do antibiotics only affect negative bacteria? explain. 13. What are the most feared diseases due to antibiotic resistance? 14. Should antibiotics be used against viruses? explain. 15. How can we avoid antibiotic resistance?2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.) 3. Please explain how the use of an Eosin Methylene Blue Agar plate can help determine the type of fermenters that bacteria are. Please explain thoroughly.
- a. After conducting the Gram staining procedures and you observed that all cells are stained with too much purple though the bacteria you fixed is Gram negative, what could be the mistake you have done? b. After conducting the Gram staining procedures and you observed that all cells are stained with too much pink though the bacteria you fixed is Gram positive, what could be the mistake you have done?Escherichia coli Mycobacterium phlei Bacillus Micrococcus subtilis luteus A B Figure 1: Agar Slant Cultures of Bacteria (Gary E. Kaiser, Ph.D.- Professor of Microbiology) . Observe and describe the colour of the slant cultures (A-D) in fig 1. ( . Define the following terms: pure culture, sterile medium, inoculum, aseptic technique, and colony. What is the name of the cultures that you used . Where they gram negative or positive Define the following terms: psychrophile, mesophile, thermophile, and hyperthermophile.a) What is morphology? b) Describe the common morphological characteristics of bacteria that are often used in microbiological studies. c) Explain the chemical and physical requirements needed for bacterial growth d) Explain the importance of using growth medium and devise a STEP-BY-STEP plan to grow bacterial cell cultures in the laboratory.