6. What does a low retention factor imply in the analysis? The pigment of interest is well carried by the mobile phase. The pigment of interest is highly attracted to the stationary phase. The mobile phase is not suitable for extracting what is desired. The stationary phase warrants modification.
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Chromatography
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- Explain how it is that SPE cartridges & disks allow analysts to more efficiently pre- concentrate analytes from samples where they exist at low concentrations.Explain the reactions involved in separation separation of benzoic acid from mixture contain 3 components 1.Benzoic acid (acidic nature pKa=4.17) 2.Naphthalene (neutral compound) 3.paraNitroaniline(basic compound pKa=12.991. Colorimetric methods of analysis are based on having a reagent that changes as a function of the concentration of the analyte. Which term best completes the sentence? pH mass color viscosity
- A 50 ml aqueous sample containing 0.015 M amine (Ka = 3.16 x 10-9) was adjusted to pH 9.0 and then extracted 24 with 100 ml of chloroform. The CHCI3-H20 partition coefficient of the amine is K=4 favoring chloroform. What fraction of the amine was extracted by the organic solvent? A: 86% B: 12% C: 95% D: 5.3% Solvent Extraction – pH Effects Fraction remaining in phase 1 after 1 extraction, q V, V,+ DV, HA N HA CHOL K [H *laq K. +[H 1 [HA], D = (acid solute) [HA], +[A ], pH more free acid, less A better extraction into organic solventWhich of the following statements is .14 ?incorrect Number of theoretical plates decreases .with increasing plate height Retention factor (k) directly proportional .to distribution coefficient (K) Large distribution coefficients of an analyte indicates that it will be retained .longer in the column .Selectivity factor always equal 1 The longer the time of separation, the .broader the zone (band) will be3. A total of 5 mL of blood samples were treated with trichloroacetic acid to precipitate proteins. After centrifugation, the resulting solution is adjusted to pH 3 and extracted with 5 mL of methyl isobutyl ketone containing the ammonium pyrolydine dithiocarbamate (APDC) complexing compound. The extract was then measured its absorbance using an atomic absorption spectrometer (AAS) at 7.283.3 nm with an absorbance of 0.502. A total of 5 mL of standard Pb solution with concentrations of 0.4 and 0.6 ppm was also given the same treatment and resulted in absorbance of 0.396 and 0.599 ppm. Determine the pb concentration in the sample in ppm units!
- functional grp analysis compound: myristic acidpls complete the table Test Reagent Observation Inference NaHCO3 Effervescence Br2 in water, dark Yellow solution 2,4-DNPH Orange solution KMnO4 Purple solution1. A two-component mixture is analyzed by paper TLC. Component A is more soluble in the mobile phase than component B. The following result is obtained. Calculate the Rifor each component and label the identity of each Frond (3-0,4)CM3D2.6cmr A (2-6-04 m = 2.2cm B C67-0.ycm=0.3cm RF (A= 2.2cm = 0.85 2. Lem spot. 2 39で Solvent Front A0-2.6m %3D 3cm Spoting Line Rf (8- 0.3cm 2. Scm = 0.12 %3D 2. Which one is the more polar component? Explain IS morC polo Because its Rf Is low 3. If a more polar solvent (mgbil phase) is used for the above experiment, the result will be: O Both component A and Component B will travel further up the plate-Distance may verur b) Only Component A will travel further up the plat c) Only Component B will travel further up the plat d) They will travel on the plate the same distance as when a less polar Choose one of the answers and explain 4. Paper chromatography works as the same principle as TLC however, cellulose paper (the same as filter paper) is used for…A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most soluble in the mobile phase and why?
- Diethylamine (C4H₁1N) and n-butylamine (C4H₁1N) are colorless liquid amine compounds, suggest chromatographic conditions (stationary and mobile phases, pH of the sample) in order to separate these compounds using IEC. Predict the retention.It has been noticed that adulteration of palm oil with carcinogenic dyes are on the rise. Use your knowledge in analytical processes to systematically process adultrated palm oil to gain qualitative and quantitative information spectroscopically about any added toxic.Explain also the basis for your protocol of choiceWhich of the following compounds will elute LAST in a reverse phase chromatography? а. b. Br с. NH2 d.