6. Use the graph below to answer the questions: a. On the graph below label the following phases: lag, log, stationary, and death b. What is the mean generation time of this culture during balanced growth? c. What is the growth rate of the culture during balanced growth? d. Is it likely that this culture will grow faster or slower in nature? Why? 250 Dry weight or cell concn 200- 150- 100- 50 O 2 4 hours 10 Time 12

6. Use the graph below to answer the questions: a. On the graph below label the following phases: lag, log, stationary, and death b. What is the mean generation time of this culture during balanced growth? c. What is the growth rate of the culture during balanced growth? d. Is it likely that this culture will grow faster or slower in nature? Why? 250 Dry weight or cell concn 200- 150- 100- 50 O 2 4 hours 10 Time 12

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Similar questions

8. A. Use Excel (or another graphing program) to draw the growth curve, In (X/X.) vs time, for bacteria grown in a 20 L suspension cell culture, given the following data: - initial concentration: 0.120 gdw cells/L Also report: - lag time: 1.5 hours - mass doubling time during exponential growth: 250 minutes - duration of exponential growth phase: 1 day (24 hours) - negligible time in the deceleration phase - 13 hours in the endogenous metabolism phase with no change in cell concentration - cell death rate with k = 0.0178 min -¹. B. What is the specific growth rate, µ? C. What is the maximum concentration of cells in the reactor? (gdw cells/L) and when does this occur? D. Other than time zero or the end of lag phase, at what time is the concentration of living cells in the reactor equal to the initial concentration of 0.120 gdw/L?
Based on the growth curve, which ONE of the following best describes what would happen if sample of culture in the presence of drug B is inoculated onto drug-free growth medium? Select one: A. Growth present, but cell count increased by at least 99.9% of the original culture B. No growth after 24 hrs C. Growth at 24 hrs D. Not possible to predict because the culture could either be alive or dead E. Growth present, but cell count reduced by at least 99.9% of the original culture
1a.) How pH of the culture medium affects the cell culture? Please give examples of low pH and high-pH culture conditions. 1b.) Describe the difference between defined and undefined cell culture media. What are the advantages and disadvantages of both of them?
3b)A plant suspension culture was used to study the effect of three types of media on the growth of the culture using batch culture approach. Figure 3.1 shows the growth of the plant suspension cultures using Murashige and Skoog (MS), Gamborg B5 and Vacin and Went media. Comment and conclude on the results obtained. Suggest which is the most suitable medium to maintain the suspension cultures with justification.
All of the following are valid measurements of growth except: A. Counting viable cells B. Estimating the weight of cells C. Determining the dry weight of cells D. Streaking for single colonies E. Measuring some cellular component F. Counting the total number of cells
1. Draw and label the parts of the growth curve if it is exponentially growing and inoculated in the same nutritious medium under the same optimum conditions. Answer and draw this completely.
(Please give clear handwritten answer) A medium containing an abundance of substrate was seeded with 20 mg/L VSS of an active mixed culture. After 8.0 hours, the VSS concentration was measured at 590 mg/L. Data indicate that there was a lag time of 0.5 hours. Assuming log growth, what is the average generation time of this culture?
Explain the statements below: 1.Animal cell cultures requires high technical skills to interpret and to regulate animal cell culturing. 2. Animal cell culture makes use of low amounts of reagents.
Using a batch culture method, the eﬀects of 3 types of media on the growth of a plant suspension culture was investigated. Figure below has shown the growth of Murashige and Skoog (MS), Gamborg B5, and Vacin and Went media in plant suspension cultures. Discuss on the findings and draw conclusions. With explanation, recommend which medium is best for maintaining suspension cultures
10. You have a 20mM stock solution of biofilm inhibitor compound X. You are asked to prepare a test tube with growth media and compound X at a final concentration of 100µM. The final volume of the test tube should be 5 ml. How much volume of stock solution and growth media should you use to prepare the test tube? Please show your work and use correct units.
What is the best descriptive match for the following culture media? A. Specific biological characteristics allow this media to select specific groups of bacteria. B. All the components are well known. C. Will contain components that can inhibit certain groups of bacteria. D. Can be used to grow difficult growing or nutritional complex bacteria. E. This media contains amino acids for growing bacteria. select 1. Synthetic media select - 2. Selective media select - 3. Enriched media select 4. Differential media select - 5. Complex media
1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.