5. Use the table below to describe under what conditions the lac operator is turned on and off. Is the lac operon ON/OFF? Condition: No lactose HIGH lactose & HIGH glucose (i.e., high ATP, low cAMP) HIGH lactose & LOW glucose (i.e. low ATP, high CAMP) What is regulating the lac operon to be ON or OFF?

5. Use the table below to describe under what conditions the lac operator is turned on and off. Is the lac operon ON/OFF? Condition: No lactose HIGH lactose & HIGH glucose (i.e., high ATP, low cAMP) HIGH lactose & LOW glucose (i.e. low ATP, high CAMP) What is regulating the lac operon to be ON or OFF?

Name: 5. Use the table below to describe under what conditions the lac oper
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Description: 5. Use the table below to describe under what conditions the lac operator is turned on and off.Is the lac operonON/OFF?Condition:No lactoseHIGH lactose & HIGH glucose (i.e.,high ATP, low cAMP)HIGH lactose & LOW glucose (i.e. lowATP, high CAMP)What i

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 11TYU

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A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcription
INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following types of mutants in E. coli. Mutant a: The map position of this mutation is in the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if large amounts of tryptophan are present in the growth medium. Mutant b: The map position of this mutation is in the trp operon. The mutant cells do not produce any enzymes coded for by the trp operon under any conditions. Mutant c: The map position of this mutation is some distance from the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if the growth medium contains large amounts of tryptophan.
2. The lac operon controls the inducible expression of genes involved in lactose utilization. Several naturally occurring mutations have been discovered that affect how the lac operon operates. Consider the following mutations: I-= a mutation in the repressor protein that cannot bind the operator Is = a mutation in the repressor protein that cannot be removed from the operator Oc= a mutation in the operator that cannot bind the repressor Detail the functionality of the lac operon with following genotypes and cellular conditions (Note if there is no superscript, there is no mutation) Genotype I, O I, OC IS, O I, O Is, Oc Lactose present No No Yes Yes No Repressed (yes/no) N VAS ves NO Constitutive (yes/no) NO NO YES Yes NO Inducible (yes/no) Yes yes NO NO YUS
1. Draw a diagram of the lac operon. PO I+P+O+Z+Y+ I+P+OcZ+Y- I-P+O+Z+Y+ Lab section (circle): lacz gene lac Y gene lacA gene I+P+O+ZY/IPO+Z+Y- 2. The promoter (P) is the start site of transcription through the binding of RNA polymerase before actual mRNA production. Mutated promoters (P) cannot bind RNA polymerase, so no mRNA is made. In the table below, make predictions about the expression of ß-galactosidase and permease for different partial diploid (F') genotypes. Do this for E. coli growth on two different types of media - one without lactose, and one with. Use a to mark where there is no enzyme produced, and a + to mark where the enzyme is made. The first genotype has all wild-type alleles, and has been filled in as an example. Genotype IPOCZ+Y/I-P+O+Z-Y+ ISP+O+Z+Y/I+P+O+Z*Y* CR 1:00 Lactose absent B-galactosidase Permease R 3:-00 + R 6:30 Lactose present B-galactosidase F 10:00 Permease
In your own words describe in detail what happens at the lac operon in each of the following situations Lactose, no glucose (high cAMP) No lactose or glucose (high cAMP) Lactose and glucose (low cAMP) Glucose, no lactose (low cAMP)
1) A. What is the difference between a repressible vs, an inducible operon? B. Using diagrams and words, describe how the Lac Operon is regulated by different levels of lactose and glucose. Be sure to show what’s happening at the molecular level, including the roles of lactose, glucose the repressor protein, cAMP and CAP.
Describe the main functional differences between the TRP and the Lac operons. Main functional differences DO NOT refer to the fact that one operon involves TRP and the other involves Lac. They also don’t include that there are different genes found in the TRP operon compared to the Lac operon or that the repressor in the Lac operon is called Lac l. Also DO NOT simply list how both operons work to answer this question.
For the next two questions, consider the fec operon of Staphylococcus madeupiddis, which encodes an iron citrate uptake system. The fec operon promoter, called "Pfec", is normally off when no regulators are bound to it (like the lac operon promoter). The activator FecA senses iron citrate and brings the appropriate enzyme to Pfec to start fec transcription when iron citrate levels are high. The repressor FecB senses free iron atoms and prevents fec transcription when free iron levels are high by binding to an operator between the promoter and structural genes. MacBook Air DII F9 888 000 F8 80 F7 F6 F5 F4 F3 & 2$ 6. 7 8. 4
All of the following statements about the repressor of the lac operon of E. coli are true EXCEPT which one? O A. The repressor is the product of a regulatory gene i The repressor binds to the operator region of the DNA of the lac OB. operon. The repressor is a protein. OC. The repressor interacts with the operator in such a way as to OD. prevent translation of beta-galactosidase. The repressor can combine with lactose to form a OE. complex that will no longer bind to the operator region. QUESTION 32 The amino acid which is a precursor of serotonin is Histidine OA. O B. Tyrosine O C. Glutamate D. Tryptophan O E. none of the above
Which of the following conditions is most likely to result in an increase rate of transcription of the lac operon? when lactose is present AND the repressor is permanently attached to the operator when lactose is present and there is high levels of glucose when lactose is absent and there are low levels of glucose when lactose is present and there are low levels of glucose
Indicate whether each of the following statements about the lac operon are true or false: Bacteria with a lac phenotype are unable to use lactose because of some defect in expressing the genes A constitutive Lac+ phenotype could mean either that the production of repressor is defective or that the operator sequence is defective The lacl gene is next to, but is not part of, the lac 1. TRUE operon 2. FALSE Bacteria with a lac* phenotype cannot grow on media containing lactose as the only sugar The structural genes of the lac operon are each transcribed as a separate MRNA Expression of the Lac Repressor from the lacl promoter is regulated by lactose
1. The lactose operon is controlled by both lactose and glucose. Fill in the following table to indicate what would be occurring under different cellular conditions Lactose absent present absent present Glucose Operon Activity (high/low) absent absent present present low high 10W high CAMP Repressor (bound/free) (high/low) bound free buind will high high low CAP (bound/free) bound free full