5) The immunoblot shows probing of samples taken from a time course after treatmentwith the proteasome inhibitor MG132. Recall that the proteasome is responsible forselectively degrading only those proteins that have been tagged with ubiquitin. Theleft indicates the antibody that is used to detect protein for each panel.P53P27AGR2GAPDHMG132 (h)0 0.5 1 2 4 8 12 24Image from Wang et al., (2019) Oncogene 38:3458-3474.a) What effect does MG132 have on each protein? What does this tell you abouteach of these proteins?●P53P27AGR2GAPDHb) How do we know that the differences we observe in the proteins is not due to un-equal loading of samples into each lane of the gel?

MG-132 is a potent proteasome and calpain inhibitor. Research studies have shown that MG-132…

5) The immunoblot shows probing of samples taken from a time course after treatment with the proteasome inhibitor MG132. Recall that the proteasome is responsible for selectively degrading only those proteins that have been tagged with ubiquitin. The left indicates the antibody that is used to detect protein for each panel. P53 P27 AGR2 GAPDH 0 MG132 (h) 0.5 1 2 4 8 12 24 Image from Wang et al., (2019) Oncogene 38:3458-3474. a) What effect does MG132 have on each protein? What does this tell you about each of these proteins? P53 P27 AGR2 GAPDH

5) The immunoblot shows probing of samples taken from a time course after treatment with the proteasome inhibitor MG132. Recall that the proteasome is responsible for selectively degrading only those proteins that have been tagged with ubiquitin. The left indicates the antibody that is used to detect protein for each panel. P53 P27 AGR2 GAPDH 0 MG132 (h) 0.5 1 2 4 8 12 24 Image from Wang et al., (2019) Oncogene 38:3458-3474. a) What effect does MG132 have on each protein? What does this tell you about each of these proteins? P53 P27 AGR2 GAPDH

Principles Of Pharmacology Med Assist

6th Edition

ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter16: Antifungal, Antiviral, And Immunizing Agents

Section: Chapter Questions

Problem 20RQ

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2. https://doi.org/10.1186/s12868-022-00692-1 (link to research) a) In the immunohistochemistry section of the materials and methods section the authors wrote “The number of positive cells in hotspot areas in ten high power fields (HPFs) in areas of demyelination and plaques in the brain stem were counted using the image analysis software (Lecia Application Suite Version 4.12.0, Welzlar, Germany).” Why were they looking at demyelination areas for this study? b) In the effect of mitoxantrone on histopathological changes in the brain section of the results section the authors wrote “Active plaques revealed inflammatory cellular infiltrates with abundant macrophages stuffed with myelin debris, an evidence of ongoing myelin breakdown.” What does macrophages stuffed with myelin debris have to do with the study?
You are interested in performing indirect immunofluorescence light microscopy to observe the localization of the catalase enzyme in the cultured HeLa cells, obtained historically from the cervical tumor of Henrietta Lacks. You were going through the lab stock and found a few primary and secondary antibodies. Which of the following secondary antibody can you use in your experiments? O All of the mentioned antibodies can be used in the experiment Goat anti-human antibody conjugated to 10 nm gold Goat-anti-human catalase conjugated to 10 nm gold O Human anti-catalase antibody conjugated to fluorescent rhodamine Goat anti-human antibody conjugated to fluorescent rhodamine
Rheumatoid Factor RF Latex Test RF is a rapid latex agglutination test kit for the detection of rheumatoid factor (RF) in human serum. RF is found in sera of patients with rheumatoid arthritis and is believed to be IgM antibodies directed against the patients own immunoglobulin G. Principle: The RF latex particles are coated with specially purified human gamma globulin. When the latex suspension is mixed with serum containing elevated RF levels on a slide, clear agglutination is seen within 2 minutes. Questions 1. Is Rheumatoid Arthritis an autoimmune disease? 2. Differentiate the different types of Immunoglobulins 3. Differentiate Rheumatoid Arthritis from Gouty Arthritis
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1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge true/false 3) PBST is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8
ANTI - STREPTOLYSIN O TITER ASO Latex Test ASO is a rapid latex agglutination test for the detection of anti – streptolysin O (ASO) antibodies in human serum. ASO antibodies are found in sera of patients in response to infection with hemolytic streptococci of group A, C or G. Streptolysin O is highly antigenic therefore patients with these infections produce specific antibodies detectable by the reagent. Questions: 1. Illustrate how latex agglutination works to bind to ASO and form agglutinates 2. What is the significance of ASO? Is ASO specific to a disease? 3. What are Todd Units? Give the normal value of ASO
Can S-layer proteins be detected by immunolabelling when a capsule is present? How do you know? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/
Serum from individuals with high levels of antibody to SARS-CoV2 has been used to treat patients with severe COVID-19. What is ONE way (there are several) that passive immunization with the antibody to the virus could help these patients? HINT: think about what opsonization with antibody could do for the innate immune response.
1. knowing that the Control is 6.22 x 10-20 moles.channel-2sec-1 and CFTR-CRISPR: 3.84x 10-20 moles.channel-2.sec how did they get the answer and explain how? Immunostaining using Quantum Dot fluorescence reveals that there are 5 CFTR channels/μm2 in the “CONTROL” and 81 CFTR channels/μm2 in the “CFTR-CRISPR”. Calculate the number of moles of Cl-transported per channel per second for both treatments. Note that there are 1x108 μm2 in each cm2
Why is a blocking buffer needed while running the immunoblotting of the PDVF membrane, furthermore why is skim mil powder often used in the buffers?
There are many immunotherapeutics now being used to immunomodulate recipients that are either fully human monoclonal antibodies or partially humanized monoclonal antibodies. Discuss whether or not they may interfere with solid phase antibody assays, CDC assays or cell based flow cytometry crossmatches.
Can a mouse infected with Bacillus anthracis generate antibodies against the S-layer? How do you know? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/