5' 3' ORF1 ORF2 ORF3 ORF4 ORF5 1. Above are pictured 2 operons, one that includes ORF1 and OFR2 and is transcribed using the top DNA strand as the template, and the other includes ORF3, ORF4, and ORF5 and is transcribed using the bottom DNA strand as the template. 3' 5' a) Complete this diagram by using arrows (4) to indicate the position and direction of the promoter(s). It doesn't matter if you draw the arrows above or below the ORFS as long as they are pointing the right way. b) Underline and label with "RBS" the ribosome binding site(s). c) How many different RNA molecules will be made from this region of DNA? d) How many different proteins will be made from this region of DNA? e) How many promoters are found in this region of DNA? f) How many stop codons are found in this region of DNA?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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5'
3'
ORF1
ORF2
ORF3
ORF4 ORF5
1. Above are pictured 2 operons, one that includes ORF1 and OFR2 and is transcribed using
the top DNA strand as the template, and the other includes ORF3, ORF4, and ORF5 and is
transcribed using the bottom DNA strand as the template.
3'
5'
a) Complete this diagram by using arrows (4) to indicate the position and direction of the
promoter(s). It doesn't matter if you draw the arrows above or below the ORFS as long
as they are pointing the right way.
b)
Underline and label with "RBS" the ribosome binding site(s).
c) How many different RNA molecules will be made from this region of DNA?
d) How many different proteins will be made from this region of DNA?
e) How many promoters are found in this region of DNA?
f) How many stop codons are found in this region of DNA?
g) What assay would you use to investigate the protein accumulation of these ORFs?
Transcribed Image Text:5' 3' ORF1 ORF2 ORF3 ORF4 ORF5 1. Above are pictured 2 operons, one that includes ORF1 and OFR2 and is transcribed using the top DNA strand as the template, and the other includes ORF3, ORF4, and ORF5 and is transcribed using the bottom DNA strand as the template. 3' 5' a) Complete this diagram by using arrows (4) to indicate the position and direction of the promoter(s). It doesn't matter if you draw the arrows above or below the ORFS as long as they are pointing the right way. b) Underline and label with "RBS" the ribosome binding site(s). c) How many different RNA molecules will be made from this region of DNA? d) How many different proteins will be made from this region of DNA? e) How many promoters are found in this region of DNA? f) How many stop codons are found in this region of DNA? g) What assay would you use to investigate the protein accumulation of these ORFs?
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