Answered: 39. The most expensive method to purify…

Science

Biology

39. The most expensive method to purify the protein is: Affinity Size exclusion RP-HPLC (reversed phase) lone exchange

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter4: Cell Structure

Section: Chapter Questions

Problem 43CTQ: Pathogenic E. coil have recently been shown to degrade tight junction proteins during infection. How...

See similar textbooks

Related questions

Q: What feature of proteins do each of these techniques utilize to identify or separates proteins: gel…

A: It is a laboratory technique for the separation of a mixture by using a solvent on a filter paper.

Q: Protein Size (kDa) Affinity to Molecule %Hydrophobicity A 22 75 85 45 C. 300 Antigen 60 A. Using…

A: Chromatography is an analytical technique; which has many subtypes based on the different working…

Q: When separated on a polyacrylamide gel, the procedure is abbreviated as SDS-PAGE (Sodium Dodecyl…

A: SDS-PAGE is a biochemical technique, which is used to separate the proteins strictly on the basis…

Q: Which of the following agents does NOT cause protein denaturation? Neutral pH Air Heat Alcohol None…

A: Introduction : Proteins are the nitrogenous organic compounds which are composed of one or more long…

Q: The purpose of SDS in an SDS-PAGE gel is to denature the proteins attach the proteins to the gel…

A: Proteins are the polymer of amino acids. They are joined by peptide bonds.

Q: Distinguish between native gel electrophoresis of protiens and SDS-PAGE of proteins in terms of the…

A: gel electrophoresis is the a biotechnological technique used to separate biological molecules based…

Q: Protein denaturation is the disruption of a protein's secondary to quaternary structures. Protein…

A: When the proteins are exposed to some acids, base, or inorganic solvent they tend to lose their…

Q: You are vworking in a commercial laboratory to purify a certain protein. Describe one of the…

A: There are four basic steps of protein purification cell lysis protein binding to a matrix washing…

Q: Please pick among these 4 Protein chromatography is typically column-based: — lon-exchange —…

A: Column chromatography is one of the most common methods of protein purification based on the…

Q: 3) Define gel electrophoresis, including its theory and application. Describe the steps of running…

A: Introduction:- Electrophoresis is a biophysical technique mainly used to separate proteins and…

Q: a protein pretreated with an sds page detergent shows two bands around 100 and 150 kD on the…

A: SDS imparts an even charge and linearises your macromolecule. Beta-mercaptoethanol breaks down the…

Q: Can someone give me examples of denatured proteins that can be brought back to native state…

A: The question asks the examples of denatured protein which can be transformed into the renatured or…

Q: Mention assays other than biuret and lowry assays that are used to detect the presence of proteins.…

A: Introduction: Protein is the major macromolecule that plays a vital role in the structure and…

Q: A Biochemist perform different experiments involving ELISA in order to determine the amount of…

A: Introduction Linear equation given as Y=0.9988 x -1.437 where Y=log A (Absorbance) and x=log [C]…

Q: The protein will lose its conformation when denatured by some factors such as a big change in pH and…

A: Denaturation: It is defined as the change of conformation protein and its physical properties. This…

Q: 1. The use of natural proteins as drugs requires compliance with certain storage and use conditions,…

A: Protein: Polymer of amino acid joined together through peptide bond removing water in a process…

Q: fter processing all of your protein samples in the laboratory, you are left with one sample to…

A: The mechanical process that is used for the separation of particles in a solution includes…

Q: Protein purification schemes typically involve several techniques. For the techniques listed below…

A: Proteins perform a wide variety of activities, which is enabled by the diversity of amino acid…

Q: Lead acetate solution is an agents of denaturation. Explain in 10 sentences the effect of each on…

A: Denaturation is the process in which the protein loses its structural configuration and as a result,…

Q: You're trying to purify a protein with the aid of an ion exchange column. On the column, the protein…

A: The most commonly used technique to separate and purify proteins, nucleic acids, polypeptides and…

Q: Which of the peptide sequences below best matches the hydropathy plot shown? 5 10 15 Residue Number…

A: Hydropathy plot is a quantitative analysis of the distribution of amino acids in a protein.…

Q: Size exclusion chromatography is a protein purification method based on size. Larger proteins exit…

A: Size exclusion chromatography: A chromatographic technique in which molecules in the solution get…

Q: Total Protein Determination Spectroscopy Values CREATE CALIBRATION CURVE? DRAWN CONCENTRATION AND…

A: The protein estimation in a sample can be done by analyzing and measuring the absorbance of the…

Q: Which of the following protein quantification techniques involves the reduction-oxidation of Cu…

A: Proteins are polymers composed of amino acids. Proteins serve several purposes in the cell and are…

Q: An extraction Buffer, EB(200 mM Trish-HCL(Ph 8), 250 mM NaCl, 25 mM EDTA, 0.5% SDS) is used for DNA…

A: The concentration of a particular substance can be represented as percentage by weight. A 1%…

Q: Compare and contrast the following protein characterization techniques in terms of the principles…

A: Mass spectrometry: a. It generates a beam of positively charged ions from the sample under…

Q: Briefly explain how the protein test for dairy products was cheated by the adulteration of the…

A: Adulteration denotes that the food product does not meet the required food safety and legal…

Q: 12. Protein catabolism can yield which of the following:

A: Protein play wide variety of essential function in our body. They provide strength and structural…

Q: Describe how the following technique work on protein purification? ammonium sulfate precipitation

A: Proteins are organic macromolecules that assist in maintaining the structural and functional…

Q: If so, how can we confirm whether the protein is purified and how to increase its purity?

A: Chromatography In 1906, chromatography was introduced by Michael Tswett to separate chlorophyll…

Q: You perform a Bradford assay. You obtain the absorbance values listed below from the BSA samples;…

A: Protein quantitation is very important process before processing protein samples for further…

Q: Explain why protein purification procedures: normally carried out 4-5°C. are

A: Crude extracts that contain the protein of interest should be refrigerated, and the recombinant…

Q: SDS-PAGE gels are useful in determining the molecular weights of proteins; however, the molecular…

A: SDS-PAGE is an acronym for sodium dodecyl sulfate–polyacrylamide gel electrophoresis. It’s a…

Q: A technique that can be used to separate proteins based primarily on their pl is called gel…

A: A sequence of techniques for isolating one or many proteins from cells, tissues, and whole organs,…

Q: A gel with a lower concentration of acryalmide will be better for separating large proteins True…

A: Polyacrylamide gel electrophoresis is a technique which is used to separate the proteins on the…

Q: Provide a one-sentence broad generalization regarding the % acrylamide one should use in SDS PAGE…

A: Hello. Since your question has multiple parts, we will solve the first question for you. If you want…

Q: Protein purification schemes typically involve several techniques. For the techniques listed below…

A: Proteins perform a wide variety of activities, which is enabled by the diversity of amino acid…

Q: Which of the following techniques can best separate a protein from other proteins on the basis of…

A: Gel electrophoresis techniques are routinely used to separate molecules based on size and molecular…

Q: Describe the principles involved in protein purification by affinity chromatography. Be thorough

A: Affinity chromatography is highly preferable to purify a protein or separate it from a mixture of…

Q: Please describe the protein purification process with the aim of purifying a protein which locates…

A: We will answer the first question since the exact one was not specified. Please submit a new…

Q: c-di-guanylate monophosphate (c-di-GMP) Polyphosphate (poly-P) Exopolysaccharides (EPS) •…

A: Bacteria are single celled organism which affects human beings by causing various diseases…

Q: 1. Describe the modes of separation in terms of their mobile and stationary phase and/or separation.…

A: Chromatography is a laboratory technique for the separation of a mixture into its components. These…

Q: The following protein quantification utilizes Cu ions except for a. Lowry b. Biuret c.Bradford

A: Protein qunatification: Measuring the protein concentration is fundamental for all quantitative…

Q: 28. The simplest method to purify the protein is: Affinity RP-HPLC (reversedphase) lone exchange…

A: Proteins are small molecules found inside cells that are essential for all structures and…

Q: present briefly the principle of size exclusion chromatography. give a brief description of the use…

A: Here, the particles are separated on the basis of their size. In this chromatography the column is…

Q: PEI is a ...... charged polyamine and will complex with and precipitate ........ /…

A: Polyethyleneimine (PEI) is a synthetic water-soluble organic polyamine polymer. These polymers can…

Q: In SIze Exclusion Chromatography, how does the instrument works that it can give data for both the…

A: a. Chromatography is an analytical technique in which compounds of interest from a mixture are…

Concept explainers

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.

Question

39. The most expensive method to purify the
protein is:
Affinity
Size exclusion
RP-HPLC (reversed phase)
lone exchange

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

SDS-PAGE gels are useful in determining the molecular weights of proteins; however, the molecular weights of are usually not reliable. protei
Using ultrafiltration, you concentrate a solution of Protein X as follows: Initial volume protein X = 300 mL Initial concentration protein X = 12 mg/mL Final volume protein X = 15 mL What's the final concentration of protein X in mg/mL?
What are the functions of the following steps in the purification and isolation of proteins: Freeze-thawing the cells • Cell press • Addition of lysozyme and DNase • Sonication • Centrifugation
Pathogenic E. coil have recently been shown to degrade tight junction proteins during infection. How would this provide an advantage to the bacteria?
2 a. You are trying to purify protein C from a mixture of proteins noted in the above Table. If you had only one type of column to choose from, which one would allow you to purify protein C with the least number of contaminants? Size exclusion column Ion exchange column Affinity chromatography using glucose as the bait Affinity chromatography using NAD as the bait Please explain why you chose the column above based upon the properties of the column AND the proteins in the Table.
Under what pH conditions can a protein not bind to the beads in a column? pH = -pKa pH = pI pH = 7 pH = pKa In size exclusion/gel filtration chromatography, the elution order is dependent upon Molecular weight Concentration Overall Charge Enzymatic Activity
Why do we use a stacking gel in SDS-PAGE
The physician ordered platinum 100 mg/m2 iv to infuse over 6 hours once 4 weeks for a patient who has a bsa of 1.66m2. (a) how many mg of this antineoplastic drug should the patient receive? (b) if the dose of cisplatin were administered in 1,000 ml d5 1/2 ns, at how many ml/hr would the iv run?
A protein gives a single band on SDS gel electrophoresis, as shown in lanes 1 and 2 below. There is little, if any, effect from adding β-mercaptoethanol(BME) to the sample; if anything, the protein runs a little bit slower. When treated with the proteolytic enzyme thrombin and electrophoresis in the absence of BME, the protein migrates a bit more rapidly (lane 3). But if BME is present, two much more rapidly migrating bands are found (lane 4). Explain these results in terms of a model for the protein.
Please provide a separation solution of combining two types of chromatography to isolate the protein A from the mixture containing protein A, B, C and D (shown as below). And then explain the related mechanisms in details. Protein A: Met-Leu-Leu-Leu-Leu-Val-Val-Val-lle-lle-lle-Leu-Leu-Leu-Leu- Protein B: Met-Asp-Glu-Glu-Glu-Glu-Asp-Asp-Asp-Glu-Glu-Asp-Asp-Glu Protein C: Met-Leu-Leu-Leu-Leu-Val-Val-Val-lle-lle-lle-Leu-Leu-Leu-Leu-Val-Val-Val- Ile-lle-lle-Leu-Leu- Leu-Leu-Val-Val-Val-lle-lle-lle-Leu-Leu-Leu-Leu-Val-Val-Val-lle-lle- lle-Leu-Leu-Leu-Leu-Val-Val-Val-Ile- Protein D: Met-Asp-Glu--Glu-Glu-Glu-Asp-Asp-Asp-Glu-Glu-Asp-Asp-Glu-Glu-Asp- Glu-Asp-Glu-Glu-Asp-Asp-Glu-Glu-Glu-Glu-Asp-Asp-Asp-Glu-Glu-Asp-Asp-Glu-Glu- Asp-Glu-Asp-Glu-Glu-Asp-Glu-Glu-Asp
Azathioprine is an immunosuppressant drug used in kidney transplants. In vivo, azathioprine is metabolized to the hypoxanthine analog 6- mercaptopurine, which is then converted into 6-mercaptopurine ribose monophosphate, the active form of the drug. 6- Mercaptopurine ribose monophosphate also inhibits de novo purine synthesis, reducing uric acid levels in the blood and urine. However, when administered to Lesch – Nyhan patients, there was no effect on uric acid levels. Explain why this is the case.
1. In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored? 2. How does SDS-PAGE separate proteins and peptides from each other? Explain. 3. Explain the Donnan Membrane Phenomenon. Why is it important for the homeostasis of the cell?