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I am having some trouble understanding this, does it have to do with something with the hemiacetal?
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- Why is agarose an ideal gel matrix for electrophoresis experiments? Because it forms a liquid at all temperatures. Because it is un-ionized and uncharged Because it is positively charged fs f6 f7 fg fg f1o $ [8 19 4 6 7 8. 4. 5 R Y F G J 2 K エ11. all of the following give a positive results in benedict test exept: O galactose xylose glucose fructose trehalose4. How important is time in the characterization of sugar in the Barfoed's test?
- 1. How does the resin facilitate purification in glutathione S-tranferase (GST) affinity? 2. Does protein stick to the resin in glutathione S-tranferase (GST) affinity? If so how do you get it off? 3. Explain how protein of interest would be purified in glutathione S-tranferase (GST) affinity?1. The main sweetener in Mackies Honeycomb is _______________1. Why is the 70:30 hexane:acetone is the more effective separation of pigments in spinach than the 50:50 hexane:acetone? Explain it thoroughly.
- What are the safety precautions in handling 10% Formalin?What are the colour changes occur to the reducing sugars after benedict test is performed? Please mention the colour change observed for each reducing sugars in benedict testWhich is the odd one out ? For the rest, explain the concept/process/technique they are involved with. agarose gel TBE TAE A260 A280 phenol
- 4.outline the processes used in the preparation of agarose gel of 1.5 concentration 5.outline the procedures /steps in gel electrophoresis 6.how do we prepare x1(concentration) TAE buffer from 50x TAE buffer20. According to the package insert, adding 2.5 mL of sterile water to a 1 g cefazolin vial yields a final volume of 3 mL. What volume of this solution would you need to obtain 200 mg of cefazolin?1. What Substances Give The Same Reaction As Sugar With Nylander’s Test And How Do You Remove It? 2. by what other method can reducing sugars be differentiated from each other and from glucose? 3. what substances interfere with the tests for fructose? for lactose?