2. The two diagrams to the right il- lustrate plots of steady-state ki- netic studies to characterize the in- teraction of heart muscle phos- phofructokinase-1 with a non-phy- siological, synthetic substrate fruc- tose-6-sulfate. Because the kcat is 08- 3.6- NH = 3.5 2.2 smaller than that for the natural 10 μΜ 20 µM 48 μΜ substrate, higher enzyme concen- trations could be used. The results show the influence of increasing concentrations of ATP on the initial > velocity of the enzyme catalyzed reaction in the presence of no AMP (•), 10 µM AMP (•), 20 µM AMP (-), and 48 µM AMP (▼). -0.8- 2.0 2.4 [ATP] (µM) log[ATP] (µM) (а) how ATP interacts with the enzyme in the case of no AMP (•). Write the reaction in words catalyzed by the enzyme for the alternative substrate, describe (b) : Explain the physical significance of the displacement of the Hill plots to the right in panel B with respect to the binding of AMP and ATP to the allosteric effector sites on the enzyme. - "A) boj (u/uļu/səjowrl) 0L
2. The two diagrams to the right il- lustrate plots of steady-state ki- netic studies to characterize the in- teraction of heart muscle phos- phofructokinase-1 with a non-phy- siological, synthetic substrate fruc- tose-6-sulfate. Because the kcat is 08- 3.6- NH = 3.5 2.2 smaller than that for the natural 10 μΜ 20 µM 48 μΜ substrate, higher enzyme concen- trations could be used. The results show the influence of increasing concentrations of ATP on the initial > velocity of the enzyme catalyzed reaction in the presence of no AMP (•), 10 µM AMP (•), 20 µM AMP (-), and 48 µM AMP (▼). -0.8- 2.0 2.4 [ATP] (µM) log[ATP] (µM) (а) how ATP interacts with the enzyme in the case of no AMP (•). Write the reaction in words catalyzed by the enzyme for the alternative substrate, describe (b) : Explain the physical significance of the displacement of the Hill plots to the right in panel B with respect to the binding of AMP and ATP to the allosteric effector sites on the enzyme. - "A) boj (u/uļu/səjowrl) 0L
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter18: Glycolysis
Section: Chapter Questions
Problem 17P
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