17-21 Is the rea (PEP) a re
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- During the process of synthesizing palmitate (C16), an acyl ACP __________. For the sake of this question, don’t treat acetyl ACP or malonyl ACP as an acyl ACP; assume the acyl ACP is a product at least 4 carbons long. Choose ALL that apply. (A) can be condensed with malonyl ACP to produce a Cn+2 b-ketoacyl ACP (B) is formed from the reduction of a trans D2-enoyl ACP(C) is a product of acetyl transacylase(D) is reduced by NADPH (E) is a substrate for b-ketoacyl synthase(F) can be condensed with acetyl ACP to produce a Cn+2 b-ketoacyl ACPWhat is the pI value of phosphoglucomutase?The mechanism involved in the reaction catalyzed by phosphoglyceromutase is known to involve a phosphorylatedenzyme intermediate. If 3-phosphoglycerate is radioactively labeledwith 32P, the product of the reaction, 2-phosphoglycerate, does nothave any radioactive label. Design a mechanism to explain these facts.
- [1-14C]Ribose-5-phosphate is incubated with a mixture of purified transketolase, transaldolase, phosphopentose isomerase, phosphopentose epimerase, and glyceraldehyde-3-phosphate. Predict the distribution of radioactivity in the erythrose-4-phosphate and fructose-6-phosphate that are formed in this mixture.10.32 Give an explanation for the Lineweaver-Burk plot for a certain enzyme-catalyzed reaction shown below. Explain why the plot is not linear 1 Vo 1 [S] no to boil an teIn order to function as an oxidative phosphorylation uncoupler, 2,4-dinitrophenol must act catalytically, not stoichiometrically. What does this mean? Identify and discuss an important implication of this conclusion.
- Seduheptulose is an intermediate in respiratory and photosynthetic pathways and plays a vital role in the non-oxidative branch of the pentose phosphate pathway. The structure of the Seduheptulose is shown below. Illustrate the two (2) pyranose Haworth projection of the said compound.384 Are there any negative allosteric regulators for glycogen phosphorylase?In the hexokinase reaction (in glycolysis) Keq caclualtion, what is the OBVIOUS problem with getting a Keq of 678? what should keq be normally? how can you EXPLAIN a Keq of 678?
- Dehalogenase enzymes catalyze the clevage of C-X bonds. One such dehalogenase catalyzes the following reaction. An active site aspartate is thought to carry out the initial nucleophilic attack that expels the chloride. Select the most likely intermediate in the reaction. (Note the stereochemistry. Refer to P11.19 in the textbook if needed.) O O A C D OE -CH₂ H H₂C™ CO₂ H B CO₂ CI N H₂O Cr CO₂ H CO₂ нотум CH3 D CO₂ five N E CH₂Human blood serum contains a class of enzymes known as acid phosphatases, which hydrolyze biological phosphate esters under slightly acidic conditions (pH 5.0): R-O-P-O3-2 + H2O --> R-OH + HO-P-O3-2. Acid phosphatases are produced by erythrocytes, the liver, kidney, spleen, and prostate gland. The enzyme from the prostate gland is clinically important because an increased activity in the blood is frequently an indication of cancer of the prostate gland. The phosphatase from the prostate gland is strongly inhibited by tartrate ion, but acid phosphatases from other tissues are not. How can this information be used to develop a commercial specific procedure for measuring the activity of the acid phosphatase of the prostate gland in human blood serum? * 1. Prostate cancer cannot be diagnosed biochemically. 2. Use tartrate to inhibit phosphatase from prostate gland and then subtract the results from the total serum enzyme activities to get an…2.7 Now that you have figured out the reaction mechanism, let's explore the action mechanism of the following drug. Notice that this molecule is composed of two chloroethylamine groups and therefore can react with two different nucleophiles. Complete the following reaction mechanism. R-N Cl CL + R'-NH₂ R"-NH₂ The above reaction is responsible for the activity of the drug. Because of its ability to react with two nucleophiles, the drug can create a bridge between two biologically active molecules and disrupt their activity. This process is known as 'cross-linking'. For example, it can react with two DNA strands and prevent their separation during replication. This will cause cytotoxicity and cell death. 2.8 Draw a scheme/cartoon to represent the general idea of chemical cross-linking described above.