16. An experiment was conducted for an enzyme catalyzed reaction and data was gathered in a table. In the experiment, the same amount of enzyme was used for each trial. The amount of substrate added, and the pH, were adjusted to see the impacts of concentration and pH on the ability for the enzyme to catalyze the reaction. Using the data table provided from the experiment (listed below), answer the following questions Amount of Substrate a. 2.0g 4.0g 6.0g 8.0g pH 3 18 24 55 55 pH 6 10 19 27 27 pH 9 35 78 92 92 pH 11 20 32 47 47 pH 13 3 10 18 18 Yield of products (number of products produced in the reaction) Looking at the data table, what was the optimum pH for this reaction? (What pH provided the highest yield of products?) b. What pH value generated the lowest yield of products? C. Briefly explain below, why was there no increase in the amount of product formed with 8.0g of substrate as compared to 6.0g of substrate? (If you are supplying more substrate, why are we not seeing more product?)
16. An experiment was conducted for an enzyme catalyzed reaction and data was gathered in a table. In the experiment, the same amount of enzyme was used for each trial. The amount of substrate added, and the pH, were adjusted to see the impacts of concentration and pH on the ability for the enzyme to catalyze the reaction. Using the data table provided from the experiment (listed below), answer the following questions Amount of Substrate a. 2.0g 4.0g 6.0g 8.0g pH 3 18 24 55 55 pH 6 10 19 27 27 pH 9 35 78 92 92 pH 11 20 32 47 47 pH 13 3 10 18 18 Yield of products (number of products produced in the reaction) Looking at the data table, what was the optimum pH for this reaction? (What pH provided the highest yield of products?) b. What pH value generated the lowest yield of products? C. Briefly explain below, why was there no increase in the amount of product formed with 8.0g of substrate as compared to 6.0g of substrate? (If you are supplying more substrate, why are we not seeing more product?)
Chemistry for Today: General, Organic, and Biochemistry
9th Edition
ISBN:9781305960060
Author:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Chapter20: Enzymes
Section: Chapter Questions
Problem 20.53E
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