(1) What is the major difference between the conventional PCR and real time PCR (qPCR) in terms of function?

Human Anatomy & Physiology (11th Edition)
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(ii)
What is the major difference between the conventional PCR and real time PCR
(qPCR) in terms of function?
SyBR green and Taqman dyes are usually utilized in real time PCR. Which dye
is more sensitive and accurate? Explain your answer.
Transcribed Image Text:(i) (ii) What is the major difference between the conventional PCR and real time PCR (qPCR) in terms of function? SyBR green and Taqman dyes are usually utilized in real time PCR. Which dye is more sensitive and accurate? Explain your answer.
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Question. What is the major difference between the conventional PCR and real time PCR (qPCR) in terms of function?

Answer : Major differences between the conventional PCR and real time PCR -

         Cnventional PCR : Conventional PCR is not quantitative , but rather qualitative. In conventional PCR , the amplified DNA product, or amplication, is detected in an end - point analysis. Conventional PCR is not ideal for rapid diagnostic use in most clinical laboratories. It has been used to detect , monitor and identify fungi from an entire set of environmental samples and is the core of molecular fungal diagnostics. The conventional PCR is based on the three simple steps required for any DNA synthesis reaction : 1. denaturation of the template into single strands; 2. annealing of primers to each original strand for new strand synthesis; and 3. extention of the new DNA strands for the primers.

        Real time PCR : also called quantitative PCR (qPCR), and it is an automated technique that allow for detection and quantification of the target material. RT-PCR  is a method frequently used to screen large cell populations , and provide rapid genotyping by HRM. IN real time PCR  , the accumulation of amplification product is measured as the reaction progresses , in real time, with product quantitification after each cycle.

                                                                                       

 

 

 

 

 

 

 

 

 

 

 

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