1) Consider the following set of data and answer the following questions: V (umolimin) ISIM 6 x 10 1x 10 2х 104 6 x 104 1.8 x 104 20.8 29 45 67.6 V (einhibitor) (umolimin) 12 15 20 24 87 28 a. Determine the Km b. Determine the Vmax c. The second set of velocities represents the rate of the reaction when an inhibitor is added. Plot these data on the same graph as above and determine the new Km and Vmax. d. Can the effects of the inhibitor be over-ridden by adding more substrate? Why? 2. The kinetics of an enzyme were analyzed in the absence of inhibitors, as well as in the presence of Inhibitor A and Inhibitor B. Using the given data below, construct or calculate the following (Make sure to label graphs with appropriate axes and equations): (S)M Vi (mmolimin) Inhibitor A 2.40 3.60 5.10 6.60 7.20 Va (mmolimin) inhibitor B 1.29 2.40 3.90 6.30 8.55 No inhibitor 3 6. 12 24 36 Vo (umolmin) 3.90 6.00 8.40 10 80 12.00 a. Write out the Michaelis-Menten equations b. Plot all of the data on one plot as Michaelis-Menten saturation curves c. Define Km and Vmax d. Mathematically determine the Km and Vmax in the absence and in the presence of inhibitors e. Determine what type of inhibitors A and B are
1) Consider the following set of data and answer the following questions: V (umolimin) ISIM 6 x 10 1x 10 2х 104 6 x 104 1.8 x 104 20.8 29 45 67.6 V (einhibitor) (umolimin) 12 15 20 24 87 28 a. Determine the Km b. Determine the Vmax c. The second set of velocities represents the rate of the reaction when an inhibitor is added. Plot these data on the same graph as above and determine the new Km and Vmax. d. Can the effects of the inhibitor be over-ridden by adding more substrate? Why? 2. The kinetics of an enzyme were analyzed in the absence of inhibitors, as well as in the presence of Inhibitor A and Inhibitor B. Using the given data below, construct or calculate the following (Make sure to label graphs with appropriate axes and equations): (S)M Vi (mmolimin) Inhibitor A 2.40 3.60 5.10 6.60 7.20 Va (mmolimin) inhibitor B 1.29 2.40 3.90 6.30 8.55 No inhibitor 3 6. 12 24 36 Vo (umolmin) 3.90 6.00 8.40 10 80 12.00 a. Write out the Michaelis-Menten equations b. Plot all of the data on one plot as Michaelis-Menten saturation curves c. Define Km and Vmax d. Mathematically determine the Km and Vmax in the absence and in the presence of inhibitors e. Determine what type of inhibitors A and B are
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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