Unknown Lab Report

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

A sample of urine was taken from a patient with kidney disease was labeled as

4) A MSA plate test was run on a sample of the organism and the results were consistent with the given results for M. Luteus. The results showed a fair amount of growth on the plate, and the color of the agar around the growth remained red. The MSA test is selective in that the salt will inhibit most gram negative organisms and select for gram positives. If there is growth and the color of the agar turns yellow around the growth, this would mean that mannitol was fermented by the organism and the acid waste released by the bacterium lowered the pH around the growth. Since there was growth and no color change, the sample is said to be gram positive and unable to ferment mannitol (negative for differential). This result was also consistent with the given test results for M. Luteus.

In this experiment, pH indicators, colour indicators, metallic ions, and Kovac’s reagent all aide in the differentiation of different bacteria under different conditions. Proteus vulgaris for example is a rapid production of urease and this is shown through the phenol red indicator, turning from yellow to pink. This experiment is usually done to distinguish from Proteus and other bacteria, which helps to isolate these bacteria since they are infectious (O'hara, et al., 2000).

In this experiment, each student was randomly assigned with a different species of gram- negative bacteria. The organism that I was assigned was Unknown #16. The identity of the gram-negative bacteria was determined to be Escherichia coli. The purpose of this report is to describe the various tests that helped develop a better understanding of the unknown microorganism in terms of the physiology, morphology, motility, and antimicrobic sensitivity it is characterized with. Indole production, hydrogen sulfide, and the colony morphology on the Eosin-methylene blue (EMB) plate, were the critical results that led to the conclusion that the organism was E. coli. In the indole production test, E. coli was one of two organisms,

Patient #1 will need several lab and diagnosis test ran on the current medication list. A CBC and culture & sensitivity test is needed for the Vantin to determine if still needed for bacterial infection. Potassium level along with renal function, bicarb and pH level should be monitored for mineral and electrolyte replacement of potassium Chloride. Furosemide (Lasix), which is a diuretic, is being administered 40mg PO daily. Electrolytes, renal function, hepatic function, glucose levels along with uric acid level should be checked before therapy begins. Also, check for decrease in potassium, sodium, calcium and magnesium. Patient #1 is on several benzodiazepines Librium 25mg Q8h, Ativan 1mg every 1-2hrs orally as needed. And Serax 15mg

For the three nutrient broth you first sterilize the loop and grab a sample of B.S and inoculated by spinning it in a circular motion inside the tube and then repeat the same method for the other two bacteria.

coli / B. subtilis, glass slides, transfer loops, a Bunsen burner, gram’s stains and alcohol) to successfully complete the experiment. Aseptic technique was used and maintained during the entire experiment by utilizing the Bunsen burner. I began by labeling two slides and adding a small drop of water to each slide. Immediately after adding water and maintaining aseptic technique, I smeared samples of bacteria to each slide, one of which received a sample of B. subtilis and one that received a sample of E. coli. My next step was to begin staining the slides with a primary stain such as Crystal Violet for 2 minutes then rinsing it with water afterward.

For this particular assignment, I was given an unknown bacteria from my lab instructor and several procedures were performed to identify the unknown bacteria. The first procedure was completing a quadrant streak on a tryptic soy agar (TSA) plate. The purpose of streaking is to isolate single colonies or colony forming units (CFU). My inoculating loop was placed into the fire of a Bunsen burner to ensure that no other bacteria were present and allowed to cool. Next the inoculating loop was placed into the TSB of the unknown bacteria and in Quadrant I I swiped from left to right from top to bottom. After flaming my inoculating loop once again, I took my loop and from the bottom of Quadrant I, and I took the bottom part of the streak and swiped

Methods: The sterile technique experiment was conducted on at SUNY College of Environmental Science and Forestry. Soil samples were gathered from both primary and secondary forest at Skaneateles Conservation Area, Onondaga County, New York. Soil samples from the primary and the secondary were used to determine the water content. In this lab, we used sterile technique to avoid any contamination of sterile media and equipment during cell culture of unwanted microbes.

Day 2: Agar plates were examined and results recorded. A gram stain was carried out for each of the microorganisms grown and examined under the microscope. The gram stain was able to indicate whether it was a gram negative or positive microbe and with further magnification it was able to indicate the shape (rod or cocci).

Escherichia coli (Gram-positive), Staphylococcus aureus (Gram-negative), and the unknown sample were heat fixed onto a slide via inoculating loop. A series of different stains (Crystal violet, Gram’s iodine, and Safranin) and alcohol are applied to the slide to reveal whether the unknown sample turned Gram-negative or positive.

Professor handed out one unknown organism to each of the students. Students had to record their own unknown ID. Used the aseptic techniques to perform the gram stain and inoculate the nutrient media(nutrient broth, nutrient agar slant, and nutrient agar plate). Only for the nutrient agar plate media used four quadrant streak method. Gram stain was distinguishing the gram-positive or gram-negative cells. Preparing of the heat-fixed slide was taking some times while air dries it. Therefore, inoculated the media of growth during the waiting time. Additionally, placed the media in the “to be incubated area.” The gram stain required four important stains and solution: Crystal violet, iodine, 95% ethanol, and safranin. Crystal violet was for the primary stain. Iodine was the mordant to form the crystal violet-iodine complex. Used the 95% ethanol to decolorize the stained slide. Safranin was the counterstain to colorize the gram-negative cell. One had to use the DI water to rinse every stain between these procedures. It was very hard to decolorize the stains, because it may

In this experiment individuals were given a plate with two unknown specimens. Each group completed a gram stain followed by a series of other test in order to identify the unknown bacteria. Each student conducted separate testing’s on one unknown in order to specifically identify the strain.

The bacteria used in this experiment is Escherichia coli which is not naturally competent. E.