Nitration Lab Report

Gloves are also very important as your hands will be dealing with the hydrochloric acid. Hydrochloric acid is a strong corrosive acid, an acid-resistant lab coat, and face mask or protective

In 2005, a chemistry teaching lab accident at Texas Tech left three undergraduate students and one graduate teaching assistant injured after a waste bottle exploded. While a student had opened the container to pour waste inside, the glass bottle exploded due to an incompatible mixture of chemicals inside. This mixture was composed of an inorganic acid and an organic solvent. The written directions for the experiment failed to omit a step which used a nitric acid wash, despite having removing that part of the procedure years prior. After the explosion, the lab was evacuated and the Texas Tech Police Department and Lubbock Fire Department responded. Fortunately, all students and staff were wearing the proper protective garments (lab coats, goggles,

The standard precautions are implemented at all times to decrease the risk of transmitting infectious agents. Assuming that all patients could carry an infection, this minimises the potential spread of HAI’s. These standard precautions include routine hand hygiene, the use of personal protective equipment, safe handling and disposure of sharps and routine environmental cleaning.4,5 In this situation, where blood is present, this is considered a biohazard. Hand hygiene must be performed before touching the patient, before and after any procedures or exposure to bodily substances and after touching the patient or any of the patient’s surroundings. The use of Personal protective equipment should be used when attending to the patient. This includes protective eye wear, a surgical mask and an apron for protection from any splashes or sprays of blood generated by the patient. Gloves should also be worn for single use only when coming in contact with open skin and bodily fluids. To minimise the spread of blood, the bystander with visibly soiled hands should also be advised to thoroughly clean them with soap and

Due to the microquantities being used, all chemicals will be disposed up with running water. Gloves, an apron, and splash goggles will be used to protect the eyes and skin.

The chemicals are stored in order of risk and state of matter to reduce the risk of danger. Acids are kept away from alkalis and are kept on the floor, surrounded by an absorbent called bunding which reduces the risk if any acid spills. Flash proof lights are used because some chemicals are very sensitive and flash proof lights reduce the risk of fire.

As the experiment was using an acid, there had to be certain precautions taken when handling and pouring out the acid. Running water was readily available in case of skin contact. Lab coats were also worn so that clothes were not damaged. As the experiment was based around heat, gloves also helped in handling hot cylinders which reached high temperature. Running water was also available in case of a skin burn.

The lab consisted of several materials such as test tubes, test tube clamps, graduated cylinder, beakers, hot plates, test tube rack, pipet rubber bulbs, porcelain test plate, medicine dropper, pipettes, thermometer, and a funnel. The solutions and temperature based materials required 0.5% amylase solution, distilled water, 0.5% starch, iodine-potassium-iodide, ice and benedict solution. While

Wear safety goggles to protect the eyes from any splashes and wearing gloves is recommended when handling acids and

If the bacteria were to spread, it could cause sickness. To prevent this from happening we are wearing disposable gloves that are to be worn only during this procedure then are being disposed immediately after the experiment is done.

For this experiment, titrations on a weak acid, acetic acid, and a buffer were performed. Acetic acid was titrated with NaOH in order to observe the half-equivalence point as well as the equivalence point. Then, the buffer and the buffered acetic acid solution prepared faced additional titration with NaOH and HCl to evaluate the differing buffering effects following the addition of a strong acid and strong base. Finally, the buffer’s buffering capacity was calculated. If the experiment were to be repeated, it would be interesting to observe the buffering effects following a titration between a weak base and a buffer instead with greater concentrations. The change in the concentration following the preparation of buffer with a weak base and its conjugate acid would pose for an interesting experiment to observe an increase in the buffering capacity.

We started with a 10.00 mL of sulfuric acid solution that was pipetted into an Erlenmeyer flask. The volume and concentration of the base must be known so that we know three of the four volumes/concentrations. The base concentration was given and the volume was obtained during the experiment. The titration resulted in the two flasks having the desired pale pink color. The volume of the base was obtained using the buret.

The technician would add 0.030mL of ferrous complex and mixed well. At the end of the reaction a report would be printed and the process repeated for 50°C, 46°C and 42°C. B2 that was done by the rest of the class. A 400mL beaker was filled ¾ full with water, with a stirring rod and heated up to 47.5°C. One test tube was contained with 9.5mL distilled water and 0.5mL of complex stock solution, another was filled with 9 mL acid stock solution and when the temperature was stable at 47.5°

The solution of 10-mL of 6M HCl and 35-mL of Acetone was properly made prior to beginning the experiment. 25-mL HCl acetone solution was then

Prior to starting the experimentation, students must wear their appropriate personal laboratory attire including, a lab coat, safety goggles, nonlatex gloves, and chemistry lab manual. After successfully conducting the experiment, students must empty contents in a waste disposal bin provided when all reactions are subsided. Students must clean plastic bag with a little water and dispose of it in a bag in the trash. To determine what combinations of chemicals are responsible for each observed changed, students may conduct a series of controlled experiments with the same quantities of chemicals. If chemicals are spilled on a student’s body or caught on fire, the best method to put it down is he or she must go to the safety shower, take off their clothes entirely and rinse off the chemicals for at least 15 minutes as recommended by the National Institute for Occupational Safety and Health (NIOSH). The same goes if chemicals get caught into the eyes or hands. Remember to carefully handle the chemicals in a cautious and efficient manner, and notify the instructor of any incidents.

The procedure given in the First Year Chemistry Lab Manual for Experiment G4 was followed with the modifications below.