Before starting this practical ensure you have sufficient PPE including lab coat, safety goggles and latex gloves. First prepare your titrant 100ml 1.0M solution of NaOH by using the top-pan balance and spatula to accurately weigh out 4g of NaOH crystals into the weighing boat. Then place the 4g of NaOH into the 250ml conical flask, add 100ml of deionised water and stir thoroughly using the magnetic stirrer. Next prepare your analyte solution of acetic acid (unknown concentration). Use the 25ml measuring cylinder to measure out 25ml of acetic acid and carefully pour into the 100ml conical flask, then using the Pasteur pipette add 3 drops of phenolphthalein (pH indicator) to the acetic acid. Next prepare your burette for use by rinsing through with distilled water catching the runoff water in the waste beaker. Once clean, use the funnel to carefully pour the 100ml 1.0M NaOH solution into the top of the burette (making sure the stopcock is closed and there are no air bubbles) filling as accurately to 0.00 as possible. If not starting on 0.00 then record actual level as your starting point. Now place the conical flask containing the 25ml of acetic acid under the burette and slowly open the stopcock allowing for a steady controlled flow of NaOH into the acetic acid. Ensure you swirl the flask containing the acetic acid as the NaOH is added. As you approach the equivalence point slow down the flow of NaOH to drop by drop. The equivalence point is the point at which the
We know that that the end point of the titration is reached when, after drop after careful drop of NaOH, the solution in the flask retains its pale pink color while swirling for about 30
If there is a chemical spill, notify your instructor immediately to prevent any further damage.
Prepare timer to start at the exact time the Alka Seltzer is dropped into the beaker of water.
The solution is then mixed with 5 ml portions of 5% sodium bicarbonate allowing separate layers to form; the bottom layer is drained out. 5 ml portions of 5% sodium bicarbonate are continuously added until the drained aqueous layer is basic with a pH
* By using the dropper and measuring cylinder, 10ml phenolphthalein was added to the test tube
mL cylinder to the beaker on the stir plate and empty it into the beaker. Place the pH probe in the beaker and record the pH in the data table. Drag the beaker to the red disposal bucket. Double-click the bottle of NaHCO3 to move it to the Stockroom counter. Repeat steps 5 and 6 for KNO3.
Measure 500ml of tap water in the 500cm3 beaker, then measure 5g of sodium hydrogen carbonate using the 50cm3 beaker and weight scale and place in the beaker of water, using the glass rod to dissolve it into the mixture.
7. Using the slider on the right hand side, add NaOH to the HCl in the Erlenmeyer flask (This action is known as titrate). Add the indicator until the color of the indicator turns a light shade of pink.
Measure and add 5cm3 of buffer solution using a measuring cylinder with the pH 3 into a test tube using a pipette and place the potato cylinders into the test tube.
When filling up the burette it is important that a funnel is used, however as the solution reaches the 0 mark it is ideal that the funnel be removed and a pipette used instead to reach the 0 mark, this is to achieve greater precision. During the experiment, it is important to swirl the flask continuously with one hand
To begin, three sets ofabout 0.3000g of KHP are weighed out on an analytical balance. Put the three sets of KHP into three separate, labeled flasks. All three sets of the KHP is then dissolved with approximately 50mL of deionized water. Next, a buret is used to start the actual titration. Buret is initially filled to 0.00mL mark with the NaOH solution, this is recorded as initial volume. Next, add 2-3 drops of phenolphthalein indicator into each of the three flasks containing KHP. A magnetic stir bar is then added to the first flask, and placed above a stir plate. Everything is positioned under the buret. Stirrer is put on medium speed and the titration can start. Slowly release the NaOH into the KHP flask. As the end point is reached, a pink color will be seen in the flask. When the lightest pink possible remains in the solution for more than 30 seconds titration is complete. The final volume is recorded, and the same steps are taken for the other two sets of KHP solution. Finally, blank titration is completed to determine deviation.
1) Pour 25 mL of the 1 M hydrochloric acid into the beaker and rinse the solid by swirling the acid around in the bottom of the beaker.
1 ml of water should be added to the first test tube and make a note. In the second test tube, 1 ml of methyl alcohol should be added. In the third test tube, 1 ml of hexane must be added. Lastly, the fourth test tube will be a control.
Measure 50ml of Sodium Hydroxide and pour in to the burette (use funnel, and remove after use).
3. Use a sterile pipette to transfer 0.1 ml of each dilution on to a MacConkey agar plate.