Digestion Lab

We set up 3 fermentation set-ups, labeling them 1, 2, and 3. Then, filled a tub with hot water and inserted the end of the plastic tubing into one of the test tubes and submerged the collection tube and plastic tubing in the tub. After that, we mixed the fermentation solutions for the other tubes, (tube 1 got 4mL of water and 3mL of corn syrup, tube 2 got 3 mL of water, 1 mL of yeast and 3 mL corn syrup, tube 3 got 1 mL water, 3 mL yeast and 3 mL of corn syrup) . We then mixed each test tube and put the rubber stoppers in the fermentation tubes. Finally, we marked the water level on each collection tube with a wax pencil to use as the baseline. Then at 5 minute intervals we measured the distance from the baseline for 20 minutes.

After complete, continue using HCl and NaOH, but now testing potato cell, liver cell, and the buffer (one at a time)

again I am going to assume this is soluble since I have not done it yet RC42 17 0 LC33 21 4 SC13 17 4Total 83 54 8

Fill a test tube about 1/3 full with cold tap water for use in step 34.

In a third test tube, put 1 mL of chutney. Repeat steps 2 and 3 with the chutney and record the results in Table 3.

Digestion is a complicated process that uses many different processes to digest food efficiently. It is necessary for not only us but for almost every organism. A major part in digestion is pH or how acidic or basic a substance is. pH helps digestion happen, the question is for the Stentor and the Rotifers at what pH ranges does their digestion occur? We will test that by using pH indicators, and observing the digestion happen under the microscope.

When experimenting this lab on your own, there are certain methods and steps you need to follow. The first step is to make sure that you have all the proper equipment needed for sufficiency and accuracy. The following equipment is needed: E.coli, Sterile L-broth, three incubators, spectrophotometer, three scientific flasks along with three cuvettes

Repeat steps 3-4: Fill 1 tube with 50% NaCl. Weigh each of the tubes/cells. Record on the chart. 3. Label a 500mL beaker with 50% NaCl and fill it with 400mL of the 50% NaCl solution.

Test tube 1 has no liver present, test tube 2-5 will have a piece of liver each 4. Test tubes 1-3 is then placed in the test tube rack 5. Test tube 4 in a beaker that is full of ice and let it cool for about 10 minutes 6. Test tube 5 is then placed in a beaker full of water on a hot plate and left to heat for about 10 minutes. 7.

1st step is to label 3 test tubes A,B,C. 2 step is to put 3 mL of homogenate into test tube A and place in ice water for 30 minutes. 3rd step is to put 3 mL of homogenate into test tube B and set it at room temperature. 4th step is to put 3 mL of homogenate into test C and place in hot bath water. After, add hydrogen peroxide to each test tube and observe what

Add the same amount of Benedict’s solution in each tube and for the ones that need iodine use the same amount.

Add 2 drops of soap to the removed test tube, and place it in the test tube holder.

Digestion is the chemical breakdown of food into smaller components that are more easily absorbed. Digestion is a form of catabolism: a breakdown of large food molecules to smaller ones. When food enters into stomach, gastric juice starts protein digestion. Gastric juice mainly contains hydrochloric acid and pepsin. The pH value of hydrochloric in the stomach is 2, as the activity of pepsin is optimal, while it will lose its activity at pH 6.5 and above. However, pepsin will regain its activity at pH of 8. In the range of pH1 to pH6.5, pepsin will be most active at pH2, and starts to decrease its

Collect to 2 large beakers both large beakers are to be filled with hot water (labtutor). Then obtain seven conical tubes these will be used to collect the levels of gas, you will also need test tube a stopper and a plastic tube (labtutor). You want to fill the conical tube to at least 50 ml of water (Cressy). Take the four conical tubes filled with water and place two in each beaker, to do this you must invert the tube and cover the release hole as to not lose any water (Cressy). Then place the beakers with the tubes in the bath so they can be at the same temperature as the bath (Cressy). Next mark all of your test tubes in number order to be sure which tube contains what concentrations and pH (Cressy). Having mixed a solution to the specifications of 2.5 ml of glucose in all tubes, 3 ml of yeast in 2 tubes of pH 5, 2 tubes of pH 9, and the single pH 7 tube, the remaining two tubes will contain no yeast as they will be negative controls. Next add 2 ml of pH buffer 3 tubes will receive pH of 5, three will receive a pH of 9 and a single tube of pH 7. Finally add pure water to make sure all test tubes have 10 ml of solution. When making the solutions

7.When water bath is ready, put each test tube into the water bath. Wait 5 minutes.