Artemia Salina Experiment

This lab was conducted with the purpose of confirming the trait of homeostasis among goldfish. During the experiment, it was recorded that the fish would increase gill movement when placed in colder water two out of the three trials. However, the results showed no significant difference in gill movement in various temperatures of water. This has very little effect on the broad field of science since our only three trials were performed and may have included human error in the trials.

effected the heartbeat rate of a Daphnia. The results of the experiment were that the

Once the experiment was concluded, aged water was slowly added to the temperature- stressed fish in order to raise the temperature of its water. This helped to return its environment back to normal conditions.

For this experiment, Tetrahymena would be starved for 0, 1, 5, 10, and 15 hours. Then, 2ml would be combined with 2ml India ink and fixed at 0, 5, 10, 20 minutes. Wet mounts would be prepared and the average number of vacuoles per Tetrahymena for twenty cells would be counted. Additionally, average cell counts per drop of fixed Tetrahymena would be taken for twenty drops. This would allow us to gain more knowledge on how exactly starvation affects the number of vacuoles and the lifespan of the Tetrahymena. This would be helpful because we observed fewer Tetrahymena in our starvation medium, but we don’t know how quickly the cells died once they were placed in this medium.

The Artemia franciscana can survive in extreme conditions of salinity, water depth, and temperature (Biology 108 laboratory manual, 2010), but do A. franciscana prefer these conditions or do they simply cope with their surroundings? This experiment explored the extent of the A. franciscanas preference towards three major stimuli: light, temperature, and acidity. A. franciscana are able to endure extreme temperature ranges from 6 ̊ C to 40 ̊ C, however since their optimal temperature for breeding is about room temperature it can be inferred that the A. franciscana will prefer this over other temperatures (Al Dhaheri and Drew, 2003). This is much the same in regards to acidity as Artemia franciscana, in general thrive in

Daphnia Magna will be used to measure how quickly dead zones are forming by eutrophication. We will use three different endpoints to measure the Daphnia Magna: Heartbeat, Mobility, and Mortality. We will have three fertilizers mixed with pond water in three separate test tubes per fertilizer with Daphnia Magna and one without ferilizer as the control. There will be three different sets each set will contain four test tubes: Three test tubes each with different fertilizers and one without as the control. We will then measure the three endpoints of the Daphnia Magna after thirty minutes which will be repeated for each set thus the whole experiment will last 90 minutes. The equipment needed for the experiment is the following:

I analyzed my Daphnia Magna everyday by observing if the Daphnia were alive or not at the end of each day. I did this by observing if the Daphnia had sunk to the bottom of the container and not moving at all for a long time. For Trial 1, SIXDAYSOX had 2 Daphnia alive while Gmark and Vitalsox only had one alive. For Trial 2, on Day 1 all the sock brands started out well and all four Daphnia survived. On Day 7 of Trial 2, Vitalsox had two Daphnia left while Gmark and SIXDAYSOX had only one alive.Also for Trial 2 one of the 4 control Daphia had died. For Trial 3, Gmark and SIXDAYSOX both had 4 Daphnia left while Vitalsox had three remaining. On Day 7 of Trial 3, Gmark had no Daphnia alive while SIXDAYSOX and Vitalsox both had one remaining.

Artemia salina are used as environmental indicator species. They are used to test changes in their environment. A. salina is sensitive to toxins, temperature, and drugs. For the experiment, cysts were put into different amounts of ethanol alcohol and their viability was measured. The hypothesis used was if there is more ethanol alcohol, then the viability of the brine shrimp is unfavorable. The brine shrimp were put into sixteen Petri dishes with the same amount of brine solution. Different amounts of ethanol alcohol were added to each Petri dish. After 168 hours, the brine shrimp were

The population dynamics of Daphnia magna are observed under three different conditions; low, medium, and high density. The effects of different population densities on the survivorship and reproduction of Daphnia are observed over a two-week period within a lab environment. Over the two week period, the numbers of parent Daphnia alive and dead are recorded daily, along with the amount of offspring produced each day. From the main parameter investigated, the net reproductive rate, the results of the experiment support that higher densities result in less successful reproduction and decreased fecundity. Values for the instantaneous growth rate of the populations also suggests that low and medium density populations allow for

The null hypothesis for this experiment is that there is no statistically significant relationship between the metabolic rate of goldfish and their exposure to light. The alternative hypothesis is that goldfish exposed to ambient light will exhibit a higher metabolic rate compared to goldfish that are exposed to dark light. Results Overall 86 goldfish (N=43) were used in the experiment. Altogether, 43 trails (2 fish per trial) were conducted in order to track the metabolic rates of the fishes. Differences in metabolic rates (with respect to light exposure) were calculated by measuring the changes of oxygen concentration in the water chambers where the fishes were placed.

For the initial set-up of the experiment, two plastic beakers, each containing approximately 200mL of fish water, were individually weighed and recorded. The selected model organism for the experiment was the Carassius auratus, most commonly known as the goldfish. Each beaker was then filled with two pairs of evenly-sized goldfish and reweighed. These weights were then subtracted by the previously recorded weights to provide an approximation of the combined weights of each paired goldfishes. An oxygen chamber with a built-in probe was then filled with 200mL of fish water. The first pair of goldfish was added, along with the 200mL of fish

However, he also acknowledged that numerous deaths were the direct result of either illnesses obtained during capture or the inability of several aquariums to properly care for the animals (Ridgway,

During this experiment many errors were made. The biggest error that was made was that we did not control the environment that the slime molds were in. We initially kept them at school in favourable conditions, where growth was good. We then took them home and moved them around the house, so the slime mold was constantly having to adapt to these environmental changes, until it could not withstand it anymore, and eventually died. Another error that was made was the fact that in transferring the slime molds, and feeding them we used only inoculating hoops, these inoculating

The last animal to examine is the goldfish. I would look at the quality of water in the fishbowl. This is going to be the first and most obvious indicator of the well being of the fish. I would ask the owners how often they change the water out and how they manage the water. A fish lives in water 24/7, and that water needs to be at a certain pH level along with other requirements like the amount on oxygen in the water. While looking at the water I would also look at the

This experiment was designed to determine the relationship between the color of light absorbed by the Lemna and the rate of reproduction. The rate of reproduction was determined by the change in the number of Lemna fronds in the beaker over time. The higher the rate of reproduction, the more effective the color of light used is on increasing the rate of reproduction. {Table 11}- Relationship between the number of Lemna fronds present in the beaker under the white light and the days passed Color of Light Average rate of reproduction of Lemna (Lemna fronds /day) Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Overall Average Overall Standard Deviation