Apple Juice Vs Pectinase Lab Report

In this experiment, 4 grams of peeled turnip was used to prepare the enzyme extract opposed to the 1 gram of turnip suggested by Fundamentals of Life Science. Along with the change to the amount of turnip used, the amount of 0.1M phosphate buffer used to prepare the enzyme extract was changed from 50mL to 30mL. The affect of temperature on enzyme activity was not

In this lab or experiment, the aim was to determine the following factors of enzymes: (1) the effects of enzymes concentration the catalytic rate or the rate of the reaction, (2) the effects of pH on a particular enzyme, an enzyme known and referred throughout this experiment as ALP (alkaline phosphate enzyme) and lastly (3) the effects of various temperatures on the reaction or catalytic rate. Throughout the experiment 8 separate cuvettes and tubes are mixed with various solutions (labeled as tables 1,3 & 4 in the apparatus/materials sections of the lab) and tested for the effects of the factors mentioned above (concentration, pH and temperature). The tubes labeled 1-4 are tested for pH with pH paper and by spectrophotometer, cuvettes 1a-4a was tested for concentration and cuvettes labeled 1b-4b was tested for temperature in four different atmospheric conditions (4ºC, 23ºC, 32ºC and 60ºC) to see how the enzyme solution was affected by the various conditions. After carrying out the procedures the results showed that the experiment followed the theory for the most part, which is that all the factors work best at its optimum level. So, the optimum pH that the enzymes reacted at was a pH of 7 (neutral), the optimum temperature that the reactions occurs with the enzymes is a temperature of 4ºC or

The scientific purpose of this experiment was to examine the rate of enzyme activity of a potato catalase when mixing 2.5 ml of potato catalyst with either 2.5 ml of H2O, 2.5 ml of 5% salt concentration, 2.5 ml of 15% salt concentration, or 2.5 ml of 30% salt concentration. It was hypothesized that the higher the concentration of salt each catalysts solution contained, the rate of enzyme activity would decrease when applied to hydrogen peroxide. This was tested by measuring (up to 240 seconds) in room temperature of 21 degrees Celsius how long it would take for each filter disk (dosed with the various salt and potato catalase solutions) to float off of the bottom of the beaker that contained hydrogen peroxide. The results of the experiment showed that enzyme activity decreased with higher salt concentrations, that being in tube four (which contained 2.5 ml of potato catalase and 2.5 ml of 30% of salt concentration) all four filter paper disks did not float within the 240 second range, having 240 seconds become the mean and 0 becoming the standard

The use of multiple test tubes and Parafilm was used for each experiment. Catechol, potato juice, pH 7 phosphate buffer, and stock potato extract 1:1 will be used to conduct the following experiments: temperature effect on enzyme activity, the effect of pH on enzyme action, the effect of enzyme concentration, and the effect of substrate concentration on enzyme activity. For the temperature effect on enzyme activity, three test tube were filled with three ml of pH 7 phosphate buffer and each test tube was labels 1.5 degrees Celsius, 20 °C, and 60 °C. The first test tube was placed in an ice-water bath, the second test tube was left at room temperature, and the third test tube was placed in approximately 60°C of warm water. After filling the test tubes with three ml of the

Enzymes react differently under different conditions and concentrations, being the most productive at the enzymes specific optimum condition and concentration. The enzyme sucrase, extracted from yeast, breaks down the complex sugar sucrose into the simple sugar glucose. Testing for sucrase’s optimum environment, multiple reactions were ran using varying amounts and concentrations of sucrose and sucrase at different pHs and temperatures. The product was then treated with Benedicts solution to visually observe what amount of glucose was present after the reaction was ran; negative results being little to no glucose present and positive results being

This lab was performed in order to discover the activity of the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close to neutral. When pH was acidic or basic, the catecholase was less effective. Also, when there was a higher concentration of potato juice and a lower concentration of phosphate buffer, absorbance of the enzyme increased.

The hypothesis was that if we investigated samples from different kingdoms, then the rate of reaction run by enzymes would change, and enzymes in animal cells would run reactions at the fastest rate, while enzymes in plant cells would run reactions at the slowest rate. The independent variable for this experiment was the samples of different kingdoms; the dependent variable was the rate of reaction run by the enzyme. To prepare a 500 ml sample, 21g of chicken liver was blended and mixed with water; and the sample of potato and the sample of an active yeast was made the same way. Then, a fermentation tube was marked from the top by centimeters and filled with hydrogen peroxide solution. Next, 1 ml of chicken liver blend was added into the tube by a pipet, and the time for 1 cm peroxide solution to be consumed was measured.

Procedure Part 1: Observe Normal Catalase Reaction Place a potato cube (3x3x3 cm) into a test tube Add 3 mL of H2O2 into each test tube Allow 1 minute for reaction to occur Record the height in cm of the bubbles Rate how rapidly the solution bubbles on a scale of 0-5 (0=no reaction, 1=slow,...5 =very fast) Part 2: Effect of Temperature on Enzyme Activity Label 3 test tubes: hot, cold, and room temperature Place potato cube (3x3x3 cm) into each test tube Place the test tube labeled hot and cold into the coordinating baths Place the test tube labeled room temperature into the test tube labeled room temperature on the test tube rack Level each test tube in place for 3 minutes After 3 minutes record the temperature of each tube Add 3 mL of H2O2 into each tube After a minute, record the height in cm of the bubbles in each tube Rate how rapidly the solution bubbles on a scale of 0-5 Part 3: Effect of pH of Enzyme Activity Label 3 test tubes acid, base, and pH 7 Place 3 mL of potato catalase into each tube Add 10 drops of vinegar to the test tube labeled acid Add 10 drops of ammonia into the tube labeled base Add 10 drops of distilled water into the tube labeled pH 7 After 2 minutes add 3 mL of H2O2 to each

Five different temperatures of enzyme (spinach extract) (5°C, 20°C, 35°C, 45°C and 65°C) were added to individual measuring cylinders -each filled with 7ml of Hydrogen Peroxide (H202). The height of foam (oxygen + water) produced by the reaction was recorded for each temperature of the catalase after 30 seconds, to find at which degrees the enzyme activity had the fastest reaction rate. The data collected from this experiment suggested that the enzyme extract had the greatest efficiency at 20 °C, and the temperatures greater displayed a decline in rate of reaction.

The results in (Table 29, Fig. 31) illustrated that as temperature of heating and time of exposure of the enzyme increase, stability of this enzyme decrease. The purified pectinase enzyme when exposed to 45°C

We tested distilled water, tap water, salt water, and sugar water. The result we recorded showed that the distilled water had the highest enzyme activity, then tap water, and then sugar, and finally salt

By looking at the tables you could see that enzymes are very specific because only test tube B and C had a reaction. The biological molecule for an enzyme is a castylist. The second experiment observe what happens to enzymes when they are exposed to different temperatures. In test tube with the avocado with ice it took 10 seconds for reaction time and foamed to the top. The test tube at room temperature took 12 second to react and also foamed at the mouth.

Enzymes are high molecular weight molecules and are proteins in nature. Enzymes work as catalysts in biochemical reactions in living organisms. Enzyme Catecholase is found on in plants, animals as well as fungi and is responsible for the darkening of different fruits. In most cases enzymatic activities are influenced by a number of factors, among them is temperature, PH, enzyme concentration as well as substrate concentration (Silverthorn, 2004). In this experiment enzyme catecholase was used to investigate the effects of PH and enzyme concentration on it rate of reaction. A pH buffer was used to control the PH, potato juice was used as the substrate and water was used as a solvent.

In the exercise # 2 we observed the effect of substrate concentration, enzyme concentration, pH and temperature on enzyme activity. All the data showed that once potato extract was added to catechol and water the reaction varied dependent on the level of catechol. As in

To find the effect of temperature on the activity of an enzyme, the experiment deals with the steps as follows. First, 3 mL if pH 7 phosphate buffer was used to fill three different test tubes that were labeled 10, 24, and 50. These three test tubes were set in three different temperature settings. The first test tube was placed in an ice-water bath for ten minutes until it reached a temperature of 2° C or less. The second tube’s temperature setting was at room temperature until a temperature of 21°C was reached. The third tube was placed in a beaker of warm-water until the contents of the beaker reached a temperature setting of 60° C. There were four more test tubes that were included in the procedure. Two of the test tubes contained potato juice were one was put in ice and the other was placed in warm-water. The other two test tubes contained catechol. One test tube was put in ice and the other in warm water. After