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1.3.1 response Essay

Satisfactory Essays

Activity 1.3.1: Student Response Sheet

PART A- Restriction Enzymes
Restriction enzymes are a tool that allows us to pinpoint human identity down to single differences in our DNA. Work through the following simulation so you can see these molecular scissors in action.

Find out more about restriction enzymes by viewing the animation and reading the article listed below.
DolanDNALearningCenter: Restriction Enzymes http://www.dnalc.org/ddnalc/resources/restriction.html
Access Excellence Classic Collection: Restriction Enzymes Background Paper http://www.accessexcellence.org/AE/AEC/CC/restriction.php

1. From what organism are restriction enzymes derived? What role do these enzymes play in this natural host?Phages. Enzymes …show more content…

Beneath each strand indicate the number of fragments that would be created and also the size of each fragment (in base pairs). Some of the pieces will have bases overhanging the edge. Use only the bases that are paired up when completing your count. For example, for a piece that looks like

ATTCAACCC
GTTGGGAA

the size of the fragment would be listed as 6 base pairs (6bp).

Person 1:

GGAATTAAGCTTATTG-AATTCTTATAG-AATTCGGGGCCCAAGCTTATG-AATTCAATT
CCTTAATTCGAATAACTTAA-GAATATCTTAA-GCCCCGGGTTCGAATACTTAA-GTTAA

Number of restriction fragments (pieces of DNA after digestion): ___4

Size of restriction fragments (in bp) - listed from largest to smallest
16;7;17;5
______________________________________________________________________

Person 2:

CCATATAG-AATTCAAGCTTAAG-AATTCGGGGGAACGTTG-AATTCAATTAATTGGG
GGTATATCTTAA-GTTCGAATTCTTAA-GCCCCCTTGCAACTTAA-GTTAATTAACCC

Number of restriction fragments (pieces of DNA after digestion): ___4______

Size of restriction fragments (in bp) - listed from largest to smallest

________8;10;13;12______________________________________________________________

PART B: Gel Electrophoresis of Restriction Fragments

After you have reviewed the principles of electrophoresis, use what you know to complete the following:
-

+

2. Place a large“+” on the end of the gel diagram where the positive electrode would go. Place a large

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