Bacteria+Lab+worksheet to jars

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Liberty University *

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220

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Chemistry

Date

May 3, 2024

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pdf

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5

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Name: _____________________________ Date: ______________________________ Bacteria Lab Worksheet Part One: Gram Staining Analysis Review the Step 1 Pre-Lab section on Gram staining and shapes of bacteria to assist you in answering the questions. 1. Observe the micrograph above containing two different bacteria on one slide. The two bacteria are Escherichia coli (E. Coli) and Staphylococcus Aureus (S. Aureus). Remember that Gram-negative are pink or red in color and gram-positive are purple or violet in color. After completing the Gram staining process, the E. Coli is determined to be Gram-negative while the S. Aureus is Gram-positive. Answer the following questions: a. What is the shape of the S. Aureus? What is the shape of the E. Coli? (3 pts)
b. What color is the E.Coli? What color is the S. Aureus? (3 pts) c. Which bacteria absorbed the counterstain (safranin)? Why? Explain in at least 3 well-written sentences. (6 pts) d. Which bacteria is more resistant to antibiotics? Why? Explain in at least 3 well-written sentences. (6 pts) 2. Gram-Positive Bacteria Analysis a. What carbohydrate comprises 90% of the cell wall? (3 pts) 3. Gram-Negative Bacteria Analysis a. In the extra outer membrane, lipopolysaccharides are considered to be endotoxins. What does this mean? (4 pts)
Part Two: Antibiotic Sensitivity Analysis Review the Step 1 Pre-Lab section on Antibiotic Sensitivity to assist you in answering the questions. 1. Observe the Petri dish above containing the bacteria Staphylococcus aureus (S. aureus). Answer the following questions based on the results from the Kirby-Bauer test. a. Rank the antibiotics from 1-5 starting with the most effective (1) to least effective (5) against the S. aureus in the chart below: (10 pts) 1. 2. 3. 4. 5.
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