Unknown Report fall 2020

.doc

School

Indian River State College *

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Course

2010

Subject

Biology

Date

Dec 6, 2023

Type

doc

Pages

Uploaded by EarlBee304 on coursehero.com

Student Name:__________________________ Identifier for your unknown (number on your starter culture):_________________ MCB 2010C Unknown Laboratory Report Due Date: (please refer to syllabus) *If you are unable to turn in the laboratory report in-class on the due date, please email me ( katherine.rawls@fscj.edu ) the report in advance if possible. Late reports will be accepted up to 2 class periods after the due date, however, point deductions will taken be as outlined in the syllabus . This report is worth 50 points toward your overall grade (45 points for report, 5 points for biochemical matrix turned in separately). Directions: 1. Fill in your name and unknown identifier at the top of this page. 2. Complete the following packet by filling in the required information into the space provided (typed or hand-written; make sure answers are legible!). 3. During the observation/conclusion section, be sure to write what you have visually observed for the observation section and what you can learn from your observation in the conclusion section. 4. Failure to FOLLOW DIRECTIONS will result in point deductions! 1

Your unknown microorganism is one of the following microbiological species. Bacillus subtilis Corynebacterium diptheriae Edwardsiela tarda Enterobacter aerogenes Enterobacter cloacae Escherichia coli Klebsiella pneumoniae Micrococcus luteus Proteus mirabilis Proteus vulgaris Salmonella typhimurium Serratia marcescens Shigella flexneri Staphylococcus aureus Staphylococcus epidermidis Streptococcus salivarius 2

Part I (20.5 points): Complete the following data tables with test observations and conclusions from experiments performed on your microbial unknown. Please use complete sentences; don’t just state “positive” or “negative” for the conclusions or you will be marked off. Note: When filling out an observation, describe what you see (i.e. lactose fermentation media has turned yellow and there is air present in the inverted Durham tube). When filling out a conclusion, describe what you can learn from your observation (i.e. the unknown is capable of fermenting lactose to an acid and a gas). Test Condition Observation Conclusions Colony pigmentation on TSA plate - use inoculated stock plate image N/A Colony form on TSA plate - use inoculated stock plate image N/A Colony margin on TSA plate - use inoculated stock plate image N/A Cell Morphology - use simple stain, Gram stain, and negative stain images (Shape? Arrangement?) – Remember to list both! N/A Gram Stain Reaction - use Gram stain image (Color?) (Gram-positive or Gram- negative?) Presence of endospores – use endospore stain image (Are endospores present? If so, what position are they?) N/A 3

Test Condition Observation Conclusions Glucose Fermentation - glucose fermentation broth, use glucose fermentation test image (Color of broth/Durham tube? Gas in Durham tube?) (Glucose fermented to acid and/or gas?) Lactose Fermentation - lactose fermentation broth, use lactose fermentation test image (Color of broth/Durham tube? Gas in Durham tube?) (Lactose fermented to acid and/or gas?) Sucrose Fermentation - sucrose fermentation broth, use sucrose fermentation test image (Color of broth/Durham tube? Gas in Durham tube?) (Sucrose fermented to acid and/or gas?) 4

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