Lab 2 Immunocytochemistry

Question 20 What test requires inoculation of rabbit serum? O ELISA O Novobiocin Agglutination O Coagulase

Question:- 1. Innate immunity is a defense mechanism that present at 2 years True False 2. ELISA test determine by antibody linked to enzyme- True False 3. Which of the following is not antifungal? Tetracycline Nystatin Flucytosine None of the above Amphotericin B

PROCEDURE 1 Time to Trace! In this procedure, you will be tracing two invaders: bacteria in the extracellular fluid and a virus that has infected cells. To keep things simple, you will trace the main pathway and the main immune response to the pathogen. Do note, however, that the actual response is much more complex, and your instructor may wish you to include more information. You are provided with hints and the number of steps you should aim to include for each organism, but you will likely want to consult your textbook for more help. After you have completed the activity, answer Check Your Understanding Questions 5 and 6 (p. 586). Start: bacteria in the extracellular fluid → bacteria damage local macrophages and damaged macrophages release that attract to the area → these cells phagocytize and bacteria and secrete chemicals to attract granulocytes called at the same time, cells called cells ingest bacteria and migrate to these cells present antigens to TĦ cells → TH cells activate…

This is homework not a test!  From NTSA case study  https://static.nsta.org/case_study_docs/case_studies/cystic_fibrosis.pdf Please help with questions 2, 3 and 4 of part four 2. "The successful use of gene therapy to cure SCID syndrome (2000) is hoped to be a permanent cure for those patients because a good copy of the problem gene was inserted into the patients' blood stem cells in the bone marrow (hematopoietic stem cells). Once white blood cells enter the blood stream they have a limited life span, on the order of a few week to months. The blood stem cells are the cells that create more white blood cells to replace those that are lost. If the gene was only inserted into the circulating mature white blood cells, the patient would only be temporarily cured until those cells were used up or died." The current gene therapy approaches to cure CF involve inserting a functional CFTR gene into the mature epithelial cells of the lungs. In light of the preceding paragraph, do you think that…

shift T ctrl caps lock tab VILION fn Quiz Submissions - Take: Unit D Practice Quiz JUDITH BIRECH (username: nj2191ne) Attempt 1 Written: Feb 9, 2023 2:20 PM - Feb 9, 2023 3:06 PM Submission View Released: Feb 13, 2023 12:01 AM Question 2 A patient had decreased mast cells. Which do you predict? K a) decreased histamine & increased inflammation Ob) increased histamine & increased inflammation 34°F Sunny /" @ Q ion x360 Laptop ment anywhere A Z W ■ 3 S E 4 alt % الالالال التاكاك R O Search 5 F T 6 8 9 ||G|H|J|K M

Please help me answer correctly the table this is my assignment in biochem lab Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 40°C , the 4th tube at 60°Cwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict’s tests.

Review Figure 1-8, "Antibody Panel, Case 1-6." In this case study, antibodies are excluded only if the patient's serum does not react with panel cells that are homozygous for the antigen. What antibodies cannot be ruled out by the antibody panel in Figure 1-8? Explain the difference in strength of reaction between cells 1,2, and 8 and cells 3,4, 9, and 10.

Question 1. Pretend you are performing an Indirect ELISA on patient to detect Streptococcus pyogenes. You did all the steps correctly, except you forgot to wash the tube before adding the substrate. How would this mistake affect your results ? Would you still be able to tell if the patient was positive or negative for S. Pyogenes?

https://youtu.be/w7aIxiZQ60g Multiplexing agglutination https://youtu.be/uWStmyJ5Qc0 This is the multiplexing agglutination. Lab report I don’t really know what to talk about, the data, conclusions and the purpose of this. Need help please

Question:- Your patient has a three month history of weight loss, night sweats, and swollen lymph nodes. He has been seen for numerous opportunistic infections. These are infections by pathogens that usually do not cause significant disease in humans. He has a history of IV drug abuse and admits to having shared used needles with other drug abusers. You suspect he has Acquired Immune Deficiency Syndrome (AIDS). The virology lab is having technical difficulties right now and is unable to test for the presence of Human Immunodeficiency virus. However, the hematology lab is able to test for the presence of different types of lymphocytes. If patient does have AIDS, which type of lymphocyte would be most affected and would the numbers of this lymphocyte be increased or decreased?

Factors which can influence antibiotic susceptibility testing (size of zone inhibition) are_________________.

Your lazy labmate needs your help! They set up a 10-ml culture of the slow-growing Myxococcus xanthus yesterday, but then they decided to stay up late playing videogames and won't be in until next Monday to recover. Whoops! They call you in lab that Friday and ask you to take an OD reading for them. You do this favor for them, and helpfully do the conversion into cells/mL, finding that their culture is currently sitting at about 3.0 x108 cells per mL. Your labmate is horrified: they won't be back in time before the cells hit stationary phase, since M. xanthus doubles every 5 hours. They beg you to make a new culture using these cells and set it up to reach the same cell density Monday morning. That's 59 hours away. If the cells usually lag for three hours upon dilution into fresh medium, what cell density should you dilute the culture to? About 1.3 x 107 cells/ml About 1.3 x 105 cells/ml About 1.3 x 106 cells/ml About 1.3 x 104 cells/ml

ACTIVITY NO. 14: ASSAYS OF ANTITHROMBIN TEST 1. Illustrate the process behind Antithrombin activity assay. Write the interpretation of results. 2. Illustrate the process behind Antithrombin antigen level test. Write the interpretation of results. 3. Explain the mechanism of the following etiologies of acquired antithrombin (AT) deficiency. A. DIC B. Nephrotic syndrome C. Heparin therapy D. Liver dysfunction

QUESTION 2 How does Complement fixation test differ from haemolytic titration assay for complement? (Answer should not be more than three lines). QUESTION 3 ELISA technique was used to assay all three pathways of complement. Which molecule will be adsorbed to the ELISA plate in each of the pathways. QUESTION 4 How can radial immunodiffusion be used to demonstrate complement function assay? (Answer should not be more than two lines) QUESTION 5 A 3-year-old child has a history of serious infections and is currently hospitalized with meningitis. The doctor suspects that he may have a complement deficiency and orders testing. A buffer that chelates calcium was added to specimen. There was a decrease radial haemolysis on agarose plate a) Which complement pathway accounted for the result obtained and how will the result in terms of hemolysis at 50 %?  b) What is hydrodynamic focusing and why is it an important feature of an instrument.

Can you please check and answer the questions that not answered, that is not graded https://www.studocu.com/en-us/ document/university-of-arkansas/microbiology/other/immunology-lab-worksheet-student/7960193/view Questions from ELISA Simulation Introduction Document (posted in Lab Module 8 folder): 1. What term is the word “antigen” derived from? 2. Where are antibodies found in the body? Plasma of the blood 3. In general, what is the cause of an autoimmune disease? 4. What kind of antibodies are present in patients with systemic lupus erythematosus (SLE)? 5. What does ELISA stand for? enzyme-linked immunosorbent assay 6. In general, what is an ELISA test used to detect? to measure the concentration of antibodies or antigens 7. In an indirect ELISA, which antibody is linked to the enzyme? (i.e the primary antibody from the blood serum or the secondary anti-human antibody) Questions from HHMI Virtual Immunology Lab Website Diagnosis…

Question:- The patient was a 6 year-old male who had been in good health without significant medical problems. In late September, he was taken to his pediatrician’s office with a complaint of a sore throat, fever, headache, and swollen glands in his neck for the past 36 hours. On physical examination, he had a fever of 100.4F, a red posterior pharynx, yellowish exudate on his tonsils, and multiple, enlarged, tender cervical lymph nodes. There were no other pertinent symptoms. Give all of the possible organisms (that we’ve studies) and why those organisms are possibilities for causing the patient’s symptoms. For the possible organisms that you gave for your answer in part a, based on the symptoms only, which of the organisms can you rule out and why? What laboratory tests can you use to rule out even more of the organisms? What results would you expect for each organism that you have not ruled out in part b. Pick ONE of the organisms that you have left, give rational why this…

Guide Question 1.What does high ASO result suggest? 2.Is ASO Test Confirmatory? 3.Describe the mechanism of streptococcal infection in immune response. 4. What are the errors in Weil-Felix test?

BONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J K

Question: A rapid COVID antigen test: may result in a false positive if you test too early. detects antibodies made against the virus in the body. can detect the COVID and flu virus simultaneously in one strip. may result in a false negative if you test too early.

Patient X- diagnosed with G6PD deficiency since birth, was prescribed with cotrimoxazole for UTI. She did not reveal her diagnosis to her physician. After 3 days, she began experiencing paleness and easy fatigability, when she went back for a follow-up, her RBC count was low.  Which sets of laboratory and diagnostic parameters must be done to monitor the patient’s response to therapy?   a. CBC, urinalysis, WBC with differentials and electrolytes b. CBC, urinalysis, serum creatinine, WBC differential, ALT c. CBC, urinalysis, fecalysis, ECG, ALT and AST, d.CBC, urinalysis, serum creatinine, ECG

a 55 -year-old man who has upon catheterization two-vessel CAD. He has adverse effects on nitrates and refused to use them again because they cause severe headaches. His medical history includes asthma and hyperlipidemia. What drug do you choose for him? Specify the name of the drug or its specific class? And Why?

Question: After completing the cells and calculating the attack rate, describe which food is the source of the food poisoning and on what you base your conclusion Please help me answer this, i was confused. Thank you!

Now that Mr. James has completed 25 days of antibiotics to treat S. marcescens in the blood, should single or double antibiotic coverage be recommended for the remainder of his 6-week course of therapy? Provide the rationale for your recommendation. Mr. Jones’ serum creatinine is 1.4 mg/dL at 1 month after discharge. At what point would you consider restarting the ACE inhibitor? Justify the use of ACE inhibitors in patients with chronic kidney disease.

This is a walk-through of Minimum Inhibitory Concentration, MIC: This tray has 8 rows, 4 for Staphylococcus aureus (SA) and 4 for Methicillin-Resistant S. aureus (MRSA). Each row has 12 compartments, or wells. The wells have different concentrations (ug/ml) of the antibiotic Oxacillin in them. From Left to Right, the concentration of Oxacillin is going down acillin concentration, pg/ml 64 32 16 8 4 2 1 0.5 0.25 0.13 0.06 0 SA dn The same amount of S. aureus broth culture is added to each well and the tray is incubated. A color change shows that S. aureus grew in a given well. MRSA What is the lowest concentration at which SA is inhibited (does not grow)? 64mg/ml What is the lowest concentration at which MRSA is inhibited? What is the MIC for Oxacillin vs SA? What is the MIC for Oxacillin vs MRSA? - Notes Comments 33°F

Write about Pfizer-BioNTech COVID-19 vaccine (Tozinameran) You must include the following information Chemistry of the drugs: Structure, physicochemical properties, (log P, pKa, & stereochemistry) and chemical properties and stability. Mechanism of action: (including the possible drug target interactions) in CoVid-19 Pharmacokinetic: Absorption, distribution (% plasma binding), chemical basis of metabolic pathways and elimination  Pharmacological properties including side effect, ADR, drug interaction, precautions, dose and dosage forms Stability and storage conditions, as well as Brand names

3:53 1 + t A 42.0 KB/S 7i ul l 62 chegg.com/homework-h 1 home / study / science / anatomy and physiology / anatomy and physiology questions and answers / asked with an image Your question has been posted. We'll notify you when a Chegg Expert has answered. Post another question. O Next time just snap a photo of your problem. No typing, no scanning, no explanation required. Get Chegg Study App Question: O Edit question Nerve Origin Movements muscles innervated Cutaneous or sensory innervation allary radial Muscilo-cutaneous ulnar median obturator Femoral nerve tibial Common fibular nerves Deep fibular nerve Superficial fibular nerve Sciatic nerve Gluteal nerves Pudendal nerves Mioinguinal nerve Lumbosacral nerves Expert Answero This question hasn't been answered COMPANY LEGAL & POLICIES CHEGO PRODUCTS AND SERVICES CHEGO NETWORK About Chegg Chegg For Good College Marketing Mobile Apps EasyBib Internships.com Advertising Choices Cheap Textbooks Cookie Notice Chegg Coupon Sell Textbooks…

It was important for Lilly to understand how the bacteria were able to cause disease in patients. The mechanism of pathogenesis by M. tuberculosis starts in the lung alveoli. The cell wall of M. tuberculosis allows it to resist phagocytolysis by the alveolar macrophages, where they can multiply. They can then induce apoptosis in macrophages, which die, and the bacteria is free to infect other macrophages. As the cycle of infection slowly progresses, the body's response to the infection is to try to wall off the bacteria in granulomas (wall of immune cells, both dead and alive, surrounding the bacteria to restrict its spread). Eventually, the bacteria can escape the granuloma and infect other parts of the lung. Transmission of Mtb Initial infection and Granuloma cavitationy replication of Mtb in macrophages and dissemination of Mtb in the lung Infected macrophages Caseating granuloma Recruitment of Innate and adaptive immune cells Infected cells undergo necrosis resulting in the…

OO HUAWEI Nova 2 Plus DUAL CAMERA Question 39 Regardless on the method of generating the vaccine or its type, there is a common fact which is? Select one: Not yet O a. The immune system will produce specific and determinant antibody for each virus answered Marked out of O b. The immune system will not react to viruses but it will react to bacterial infections only 1.00 P Flag O C. The immune system will produce several antibodies for one type of virus question O d. The immune system will produce a general antibody to any invading virus e. The immune system will engulf any virus Question 40 HETEROCHROMATIN refers to O a. Regions of the genome that is unfavorable for gene translation Not yet answered Ob. Region of the genome that is favorable for gene replication Marked out of O C. Regions of the genome that is favorable for gene transcription 1.00 Od. Regions of the genome that is unfavorable for gene transcription P Flag question O e. Regions of the genome that is favorable for gene…