07 Lab 5 Movement of Molecules

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Lone Star College System, North Harris *

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1406

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Biology

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Apr 26, 2024

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pdf

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Lab 5: Movement of Molecules 19 Lab 5: Movement of Molecules The plasma membrane of a living cell regulates the traffic of molecules in and out of the cell. Much has been learned about the movement of substances in and out of the cell by the use of artificial membranes. In this lab, dialysis tubing will be used to simulate a plasma membrane. Like a living membrane, it is selectively permeable. It has tiny pores that limit the passage of substances based on size. Anything larger in size than a monosaccharide may not be able to cross the “membrane”. Unlike a real membrane, it does not discriminate among nonpolar, polar, and ionic substances. _____________________________________________________________________________________________ In this lab, you will investigate the passive movement of molecules. This process requires a concentration gradient. Although there are many factors that affect the rate of movement, only size of molecules and the steepness of the gradient will be studied. This lab has 6 objectives: Observe Brownian movement. Determine the effect of solute concentration on the rate of osmosis. Investigate the selective permeability of an artificial membrane. Determine the effect of molecular weight on the rate of diffusion. Observe osmosis in plant cells. Observe osmosis in animal cells. P ROBLEM O F THE D AY How does size of molecules and steepness of concentration gradient affect movement of molecules? A. BROWNIAN MOVEMENT In 1827 Robert Brown, a Scottish botanist, observed that small pollen grains suspended in water exhibit a peculiar random vibrating movement. He erroneously thought that this motion was due to living activity. Instead, this motion, called Brownian movement , is due to random collisions resulting from kinetic energy. 1. Open the “Lab 5” folder on the desktop and click on the “Brownian Movement” file to observe India ink magnified 1000 times. India ink is a suspension of tiny particles of carbon in water. 2. Compare the movement of large particles with smaller ones. Do not confuse Brownian movement with bulk flow. Bulk flow occurs when all the particles of carbon are swept along in one direction. 3. Turn to section A of the Lab Report and answer the questions. B. THE EFFECT OF SOLUTE CONCENTRATION ON THE RATE OF OSMOSIS Problem 1: Osmosis only occurs when there is a concentration gradient. It is the diffusion of water across a selectively permeable membrane. What effect does the concentration gradient have on the rate and direction of osmosis? To answer this question, you will use pieces of dialysis tubing that have been made into small bags by tying off the ends. Each bag contains a 10% sucrose solution. The bags will
2 0 Lab 5: Movement of Molecules be immersed into beakers containing solutions of various concentrations: Beaker 1 = distilled water Beaker 2 = 10% sucrose solution Beaker 3 = 20% sucrose solution Beaker 4 = 40% sucrose solution Predictions: Predict what will happen to the 10% sucrose bag when placed in each beaker. Complete the Predictions Table (3rd page of Lab Report), indicating whether the bag will lose weight, gain weight, or not change. Procedure : This experiment will be conducted as a class. It will require 4 teams to gather data. 1. Lab Tech from each team, obtain the following 2 items from the side counter. a. a beaker containing 100 mL of your assigned solution b. a bag containing 10% sucrose 2. Technician, in this experiment, you will now be referred to as the “weigher.” Blot the bag dry, weigh it, and record its weight to the nearest 0.1 gram in Results Table (3rd page of Lab Report). 3. Once all 4 teams are ready, the 4 bags will simultaneously be dropped into their respective beakers. For the next 40 minutes, at 10 minute intervals, the “weigher” for each group will remove the bag from the beaker, blot it dray, weigh it, and report the data so that everyone can record it. On the instructor’s signal, “weighers” will return their bags to the beakers. 4. For each 10 minute reading, calculate the change in weight ( W) by subtracting the initial weight (0 minutes) from the weight for each 10 minute reading. Record your data in the Results Table (3 rd page of Lab Report). 5. Once all measurements have been made and recorded, use all the data to graph the results (4th page of Lab Report) and answer the related questions in part B of the Lab Report. ____________________________________________________________________________________________________________________________________________________________ C. SELECTIVE PERMEABILITY OF AN ARTIFICIAL MEMBRANE Problem 2: How does the size of the solute particle affect its ability to diffuse across an artificial membrane? A dialysis bag containing a solution of 10% starch, 10% protein, 10% NaCl, and 10% glucose (for a total solute concentration of 40%) will be immersed into a beaker containing distilled water. Predictions: 1. Is the solution in the bag hypertonic, hypotonic, or isotonic to the distilled water in the beaker? _________________________ 2. Will osmosis occur? _____________________ 3. If so, will water enter or exit the bag? _____ 4. What will happen to the weight of the bag? __________________________________ 5. Predict which substance(s) will diffuse out of the bag. _____________________________ 6. Now, predict which substance(s) cannot diffuse out of the bag. __________________ Procedure: 1. Obtain a dialysis bag containing the solution described above and a beaker containing 100 mL of distilled water. Rinse the bag off thoroughly with tap water and then blot it dry. 2. Determine the initial weight of the bag. Record this weight to the nearest 0.1 g in the space provided below. 3. Obtain 4 clean white-coded test tubes and using a 3 mL pipette, add 3 mL of dH 2 O from the beaker to each tube. Set these samples aside. They will serve as negative controls for the chemical tests performed later. 4. Now, place the bag into the beaker of distilled water for 30 minutes.
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