Your HRP sample had a concentration of 2.0 mg/mL and you added 10 uL to a tube containing 10 uL of 2X Sample Buffer. You mixed this well and then heated at 75C for 5 min. Then you added 10 uL of this solution to a well of a gel. How much protein did you add to that well?
Q: Enter your answer in the provided box. A constant current is passed through an electrolytic cell…
A:
Q: ОН Br
A:
Q: 6) C,H,X in this compound 100 80 MASS SPECTRUM 0.0 40 120 80 m/z NIST Chemistry WebBook…
A: Note : M:M+2 ratio is 1:1. Therefore the compound is having bromine atom. m/z = 43 belongs to propyl…
Q: Calculate the magnetic moment of Mn in K4[Mn(NCS) 6].
A: The complex given is K4[Mn(NCS)6].
Q: Fill in the molecular orbitals for the H2 molecule. How do you describe the highest occupied…
A: Fill in the molecular orbitals for the H2 molecule. How do you describe the highest occupied…
Q: Consider the compounds below: (i) (ii) (iii) Ho (iv) OH Which molecule is most water soluble? O (1)…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: The solubility-product constant for Ce(IO3)3 is 3.2 x 10-10. What is the Ce³+ concentration in a…
A: Given: Concentration of Ce3+ = 0.0450 M Volume of Ce3+ solution = 50.0 mL = 0.050 L Concentration…
Q: What is the pH of a solution prepared by mixing 50.00 mL of 0.10 M NH3 with 20.00 mL of 0.10 M…
A:
Q: Select the meta-substituted dibromobenzene Br Br Br Br & § Br Br B C A B C D A Br D Br
A:
Q: A flask contains 600 mL of chlorine gas at 560mm Hg. If the volume of chlorine gas inside the flask…
A:
Q: The gre ench chemist Antoine Lavoisier discovered the Law of Conservation of Mass in part by doing a…
A:
Q: What is the mole percent of ethanol (C2H5OH) in the 180 proof vodka, which consists of 71.0 g of…
A:
Q: What is the expected product A for the following Claisen condensation reaction? 1. NaOEt H₂C. A OEt…
A: In this question we have to tell the product of the Claisen condensation reaction.
Q: E. REAGRRANGING EQUATIONS. If you want to calculate the amount of energy transferred the equation…
A: Answer: As given, E=mC∆T Here: E=energy transferredm=mass of waterC=specific heat of…
Q: A mixture of pure BaCO3 and pure Na2CO3 weighs 1.000 g and has the total neutralizing power of 15.37…
A: the solution is shown below
Q: 2. Describe the underlying principles behind the complexometric determination of water hardness.…
A: Water is pure when nothing is added to it, but it becomes hard when the calcium and magnesium ions…
Q: If a gas with a volume of 10mL and a pressure of 236 torrs inside a dewar flask is opened to an…
A:
Q: What is the shape of the chlorine orbital that contains only one electron
A:
Q: A two-component mixture is analyzed by paper TLC. Component A is more soluble in the mobile phase…
A: Note : Compound A is more soluble in mobile phase. Therefore compound A is less polar and having…
Q: NaOH + HCl ---> NaCl + H2O 123.60 mL of 3.90 M aqueous NaOH is required to titrate 346.00 mL of an…
A: Given reaction is : NaOH + HCl ---> NaCl + H2O Molarity of NaOH = 3.90 M Volume of NaOH =…
Q: V. Mechanism. 1. Consider the following reaction. CH₂OH Ho Br OCH₂ 3. Draw out a complete electron…
A: 1 a. Methanol is polar protic solvent. The prefered mechanism is sn1. Here rearranged product is…
Q: Given the equation in a basic medium: MnO4¯ + SbH3 ---> MnO2 + Sb Choose all the statements that…
A: Here we are required to find the balance the redox reaction by ion electron method.
Q: Pentane has a [Select] hexane has a [Select] Butane has a [Select] pentane has a [Select] pentane is…
A: Here we are required to compare the solubility and boiling point of various organic compound
Q: (a) Draw the condensed structural formula, and give the name for the dipeptide Pro-Asn. (b) Draw the…
A:
Q: calculate the pH of a solution that is 1.00 M CH3COOH (ka = 1.0 x 10^-5) and 1.00 M CH3COONa
A: Given: Concentration of weak acid CH3COOH = 1.00 M And the concentration of CH3COONa = 1.00 M
Q: How many valence electrons do you have to account for when drawing the Lewis dot structure of PCl5?
A: Valence electron of phosphorus is five and valence electrons of chlorine is seven
Q: Example 7. Which one of Fe2+ and Fe3+ ions is more para- magnetic and why? Explain. Solution T.3+ 2+…
A: Ion with more number of unpaired electrons is more paramagnetic than the ion with less number of…
Q: How many KJ of energy are released when 6.39 g of Fe reacts with excess O₂? Given the following…
A: Given : Mass of Fe = 6.39 gm 4Fe + 3O2 -----> 2Fe2O3. ∆H = -1625 KJ
Q: 1:34 1 v4 nk MASS SPEC LAB spectra o... 1) A Hydrocarbon - aliphatic or aromatic MASS SPECTRUM 100…
A: Compound 1 is hydrocarbon. Molecular ion peak at 114. m/z = 43. Therefore the compound is n-octane.…
Q: Determine the correct pressure (P1) of the dry hydrogen gas.
A:
Q: 2. One way to determine the concentration of lead (II) in water is by precipitating it via titration…
A: The relationship between Molarity(M) , volume (V) and stoichiometric coefficient (n) of any two…
Q: Chemistry 1a. Draw the structure of dehydroascorbic acid. 1b. Draw the structure for ascorbic acid.…
A: Ascorbic acid is an acid and dehydroascorbic acid will be the dehydrated form of ascorbic acid. In…
Q: C + Lo |||
A: 1.Ethyne is alkylated using NaNH2 and methyl iodide. Methyl acetylene is subjected to hydroboration…
Q: An allylic free radical halogenation occurs to give the major product shown. Note NBS is a source of…
A: Detail mechanistic pathway is given below
Q: What mass of aluminum metal would absorb 250.0 kJ when it melted at its melting point? The molar…
A:
Q: Arrange the following molecules in order of increasing average molecular speed. Ar at 331K, H₂ at…
A: Below attached file showing the details answer. All the best.
Q: The acid-dissociation constant (Ka) of hydrocyanic acid (HCN) at 25.0°C is 4.9 × 10-10. What is the…
A:
Q: Calculate AG and the equilibrium constant for the following reaction at 298 K: Zn²+ (aq) + Ni(s) →…
A:
Q: Consider the following reaction: 2- bromooctane reacted with Sodium tert-butoxide complete reaction…
A: 2-bromooctane + sodium tert-butoxide --> ->Sodium tert-butoxide has more basic character than…
Q: For how many minutes would 10.00 A of current need to flow to produce 5.0 x 104C? O a. 5000 minutes…
A:
Q: 600.0 mL of a gas is at a pressure of 8.00 atm. What is the volume of the gas at 2.00 atm?
A: Given : Initial volume(Vi) = 600 ml Initial pressure (Pi) = 8.00 atm Final pressure (Pf) = 2.00…
Q: The pH of an acidic solution is 2.41. What is (OH-)
A:
Q: The pH of a 0.64M solution of acrylic acid (HC3H₂CO₂) is measured to be 2.22. Calculate the acid…
A:
Q: What is the difference between ideal gas and real gas laws?
A:
Q: For the aqueous [Co(NH₂) complex K = 1.58 × 10³5 at 25 °C. 3+ Suppose equal volumes of 0.0012 M Co…
A: Answer: 3.7×10^-37 M
Q: Structural form of Fischer projection and Haworth projection of D-Mannose
A:
Q: c. Describe the coupling pattern (i.e. doublet, triplet, doublet of doublets, etc.) for the peak at…
A: Solutions- Coupling pattern is the doublet, triplet, the 1H NMR spectrum was obtained on a 300 MHz…
Q: VII. Predict the product(s) of the following cleavage reactions. HI H₂O 1. HI 2. H₂O хо HI H₂O 3.
A: 1.Ether react with alkyl halide by Bimolecule nucleophile substitution reaction and give…
Q: Based on the thermodynamic properties provided for water, determine the energy change when the…
A: Answer: In this cooling process water will lose its energy via 3 step process: 1. Vapor will be…
Q: Review Reactions 1. MCPBA 2. H30+
A:
Step by step
Solved in 4 steps
- You have a 10 mg/ml stock solution of ethidium bromide (EtBr) and need to dilute it to 0.2 micrograms/mL in gel running buffer and the gel itself. Your gel volume is 50 mL and you need 400 mL of the running buffer. How much of the concentrated stock solution do you put in the gel and the running buffer?Below is the procedure described for determination of paracetamol according to BP: "Weigh and powder 20 tablets. Add a quantity of powder equivalent to about 0.35 g paracetamol to 50 ml of 0.1 M NaOH, dilute with water to 200 ml and shake for 15 min. Add sufficient water to make up 250 ml. Filter the solution and dilute 5 ml of the filtrate to 100 ml with water. Dilute further 10 ml to 100 ml. Measure the absorbance at 257 nm. The A," following is correct? 715 at 257 nm". Which of the O a. An analyst can take 0.37g of the powdered tablets instead of 0.35. O b. An analyst can replace NaOH with HCI OC. An analyst can skip the shaking step O d. B andC O e. All of the aboveBelow is the procedure described for determination of paracetamol according to BP: "Weigh and powder 20 tablets. Add a quantity of powder equivalent to about 0.35 g paracetamol to 50 ml of 0.1 M NaOH, dilute with water to 200 ml and shake for 15 min. Add sufficient water to make up 250 ml. Filter the solution and dilute 5 ml of the filtrate to 100 ml with water. Dilute further 10 ml to 100 ml. Measure the absorbance at 257 nm. The A = 715 at 257 nm". Which of the following is correct? O a. An analyst can take 0.37g of the powdered tablets instead of 0.35. O b. An analyst can replace NAOH with HCI C. An analyst can skip the shaking step d. B and C e. All of the above
- Below is the procedure described for determination of paracetamol according to BP: "Weigh and powder 20 tablets. Add a quantity of powder equivalent to about 0.35 g paracetamol to 50 ml of 0.1 M NaOH, dilute with water to 200 ml and shake for 15 min. Add sufficient water to make up 250 ml. Filter the solution and dilute 5 ml of the filtrate to 100 ml with water. Dilute further 10 ml to 100 ml. Measure the absorbance at 257 nm. The A, = 715 at 257 nm". Which of the following is correct? O a. An analyst can take 0.37g of the powdered tablets instead of 0.35. O b. An analyst can replace NaOH with HCl O c. An analyst can skip the shaking step O d. B andC O e. All of the above3. You are loading a protein gel that holds up to 50ul. You need to load 25ug of protein sample that is at a concentration of 8 ug/ul. You are mixing your sample with a 4x Laemmli Buffer prior to loading. How much of each component will you mix and how much of total volume will you load in the well? You-a- EL COL DDOT.no:You need to make a 1% agarose gel in 1X SB buffer. How would you make 500 ml of 1X SB buffer from a 20X SB stock? How many grams of agarose would you use in a 50 ml gel?
- From a 10-mL sample, a 1-mL aliquot was taken and diluted to 100mL. From this, a 5-mL aliquot was taken and diluted to 20mL.The final 20mL was found to have a concentration of 0.004M analyte X.What is the concentration of analyte X in the 10-mL sample?A typical protein contains 16.2 wt% nitrogen. A 0.500-mL aliquot of protein solution was digested, and the liberated NH3 was distilled into 10.00 mL of 0.021 40 M HCl. Unreacted HCl required 3.26 mL of 0.019 8 M NaOH for complete titration. Find the concentration of protein (mg protein/mL) in the original sample.A 1.0-g fat was titrated against a 0.500-M KOH and consumed 13.0-mL of the titrant. A blank analysis was performed, and a 20.0-mL of the titrant was used. Calculate the saponification number of the fat.
- Submit a clean, dry, and properly labeled 50-mL reagent bottle for your unknown solution. Pipet 20.00 mL of the sample in 250-mL Erlenmeyer flask. Add 5 mL of buffer and 5 drops of indicator. Titrate the solution until it turns light blue. If the titration consumes more than 50 mL of the titrant, dilute the sample accordingly. Compute for ppm of CaCO3 using the following table. Mean Molarity = 2.487 x 10^-3 MBlank correction = 0.015 Formula = V (mL) titrant x Mean Molarity of Titrant x Molecular weight of CaCO3 x 1/V (L) sampleAcetylcholine esterase, 3.0 mg, was dissolved in 10.0 mL solution. From this solution, 0.60 mL was added to the sample tube, brining the volume to a total of 3.0 mL. How many mg of acetylcholine esterase did you have in the sample tube?22 g of potato salad was added to 60 mLs of buffer and blended. Three (1/10) dilutions of the mixture were made and 1 mL was plated from the third dilution. The resulting count was 89 colonies. What was the original concentration of bacteria per gram of potato salad?