You want to study the oxygen binding ability of hemoglobin at pH 7.4. You decide to use a phosphate buffer. How would you make up 600 ml of 50 mM phosphate buffer, pH 7.4, starting with 3 M Na3PO4 and either 2 M HC1 or 1 M NaOH? Show all calculations.

Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter15: Additional Aqueous Equilibria
Section: Chapter Questions
Problem 105QRT: Apatite, Ca5(PO4)3OH, is the mineral in teeth. On a chemical basis explain why drinking milk...
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You want to study the oxygen binding ability of hemoglobin at pH 7.4. You decide to use a
phosphate buffer. How would you make up 600 ml of 50 mM phosphate buffer, pH 7.4, starting with
3 M Na3PO4 and either 2 M HC1 or 1 M NaOH? Show all calculations.
Transcribed Image Text:You want to study the oxygen binding ability of hemoglobin at pH 7.4. You decide to use a phosphate buffer. How would you make up 600 ml of 50 mM phosphate buffer, pH 7.4, starting with 3 M Na3PO4 and either 2 M HC1 or 1 M NaOH? Show all calculations.
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