Which of the following is false about cyclin-cdk complexes? Cdk's do not have to bind to cyclin proteins for complex to be active. OCdk/cyclin complexes phosphorylates proteins required to trigger next cell cycle phase. Process acts as molecular brakes to ensure cell is ready to continue with cell cycle. OCyclin concentrations increase gradually, but cdk must be phosphorylated by specific kinase for complex to be active |0|0
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- Which of the following would result in cell cycle arrest? O phosphorylation of Rb by G1-Cdk phosphorylation of p53 O phosphorylation of helicase by S-Cdk phosphorylation of M-Cdk by CAK phosphorylation of APC by M-CdkIn mammalian cells, different cyclin-CDK complexes regulate progression of cells through the cell cycle. Which of the following would be true during the cell cycle? Select all that apply Cyclin D phosphorylates and activates MCM protein, a helicase Cyclin B phosphorylates and breaks down the nuclear lamina OThe anaphase promoting complex, or APC/C is phosphorylated and activated by cyclin B Cyclin D helps promotes passage through the restriction pointThe cell cycle contains a series of events that control the division of cells. It is controlled by which of the following: P. dephosphorylation of cyclin-dependent kinases (S) Q. binding of CDK inhibitor proteins (R) R. cyclin synthesis and degradation (P) S. phosphorylation of cyclin-dependent kinases (Q) R and S P, Q, R, and S Q and R P and Q
- The cell cycle contains a series of events that control the division of cells. It is controlled by which of the following: P. dephosphorylation of cyclin-dependent kinases (S) Q. binding of CDK inhibitor proteins (R) R. cyclin synthesis and degradation (P) S. phosphorylation of cyclin-dependent kinases (Q) Q and R P, Q, R, and S R and S P and Q O(Q) During M-phase of cell cycle, active M-Cdk phosphorylates several target proteins in order to regulate their activities. Identify TWO specific examples of such target proteins that are phosphorylated by active M-Cdk, and explain how each of them affects the cell (i.e. their respective biological significance). i) target protein phosphorylated: _______________________________ Biological Significance: ii) target protein phosphorylated: _______________________________ Biological Significance:Diagram (just use arrows in the same way you diagrammed in a former test a stimulus leading to the activation of PKC, for example) in as much detail as possible what happens to a mammalian cell when it is irradiated, leading to when the cycle stops due to DNA damage. Include and name the famous mammalian checkpoint protein known by its molecular weight, as well as name another protein it activates. This other protein's function can be described by a 3 letter acronym that contains the letter K. Mention the acronym. 1.
- The following figure shows how the activities (y-axis) of Cdk-Cyclin B complex and the APC complex change through the cell cycle. Active Cdk1-Cyclin Inactive Cdk1-Cyclin Active APC A B A B timeWhich situation would be the most likely result of a mutation in a eukaryotic organism that causes it to produce less p21 protein than normal? Cells with radiation-induced DNA damage would continue through the cell cycle without repairing the damaged DNA. O Cells with incomplete DNA replication would continue through the checkpoint. Cell replication would be slowed due to an increase in active G1/S cyclin-dependent kinase. Cells would not respond to growth factors. O Cells would have a hypersensitive response to growth factors.B. Cyclin-dependent Kinase 1. What are the proteins that determine the forward momentum of the cell cycle? 2. What essential nutrient (aside from protein) is needed so that the cycle may grow or develop? 3. What do you infer, in what conditions will the cell may advance the cell cycle? Explain briefly. CDK Activity G₁ G₁ Checkpoint The cyclin-CDK system checks for favorable environmental conditions and damaged DNA. DNA Replication Checkpoint The cyclin-CDK system checks for stalled replication machinery and damaged DNA. Time G₂ G₂ Checkpoint The cyclin-CDK system checks for unreplicated or damaged DNA. Metaphase Checkpoint The cyclin-CDK system checks that all chromo- somes are attached to spindle microtubules.
- Cell cycle checkpoints are surveillance mechanisms that monitor the integrity and fidelity of the major events of the cell cycle. Which of the following are true about cell cycle checkpoints? Select all that apply The M phase checkpoint examines whether all sister chromatids are correctly attached to the spindle microtubules Progression through checkpoints is determined by the activation of cyclin dependent kinases bound to cyclins The G2 checkpoint is also called the restriction checkpoint O If a cell passes the G1 checkpoint, it irreversibly commits to complete the whole cell cycle and divideAfter DNA damage (e.g. caused by X-ray exposure) in eukaryotic cells, the cell cycle can be arrested by the stabilisation of the protein which drives the transcription of the gene, whose protein product interacts with the G1/S-Cdk and S-Cdk complexes. O PDGF, acetyltransferase O phenylalanine hydraxylase, PDGF O p53, acetyltransferase O p53, p21 O p21. p53Which structure will form during the cytokinesis of a plant cell? septum cell plate cleavage furrow Which choice is an example of a product that is not a negative regulator protein. p21 RB1 p53 Cdk Which cell cycle checkpoint occurs during the mitotic phase of the cell cycle? M G2 G1 The mitotic phase of the cell cycle consists of cell growth DNA replication mitosis and cytokinesis The formation of a cleavage furrow only occurs during the cytokinesis of bacterial cells plant cells animal cells Single-celled eukaryotic organisms reproduce by the cell division process called reduction division mitosis meiosis binary fission In which stage of the cell cycle do cells that are not going to divide further are shunted into: S G1 G0 G2 Human sperm or egg cells contain one set of chromosomes which is a configuration known as __________ or ________. diploid, 2n diploid, n haploid, 2n haploid, n…