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What is with broth that made it an optimum specimen for Francisco Redi for his experiment to prove or disprove spontaneous generation?
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- How would the presence of endospores in Louis Pasteur's nutrient solutions have affected his conclusions about spontaneous generation?In order to have a well-designed experiment, why would it be important to include the beaker of yeast and water.Jessie is working with a 100% stock concentration of Clorox. She decides to add 100 µL of bacteria to a tube containing 3 mL of nutrient broth and then adds 400µL of the Clorox stock to the tube as well. What is the unknown concentration of the tube?
- Jordan is working with an 60% stock concentration of Windex. She decides to add 100 μL of bacteria to atube containing 3 mL of nutrient broth and then adds 1000μL of the Windex stock to the tube as well.What is the unknown concentration of the tube?Was there any difference in the amount of gas produced by yeast in room temperature tapwater compared with yeast in tapwater at 35-40 degrees Centigrade? Explain why this might be so.Why would farmers be concerned about nitrifying bacteria?
- Use the following diagram to explain how Pasteur’s swan-necked flasks prevent contamination of sterile broth the flasks.Describe what happens to the sterile broth (a) after it has beenallowed to cool as in (b). What happens to the broth after theflask has been tipped enough to let the broth come in contactwith the dust and microorganisms and is tipped back as in (c)?Which among the group would be affected when excessive Zn++ is present in the medium? Provide two (2) corrective actions to resolve this error.Answer the following questions in three sentences only: What evidences supported spontaneous generation? How was spontaneous generation disproved?
- propose a hypothesis regarding the organisms (staphylococcus, escherichia, pseudomonas, and bacillus) that will be resistant to the most disinfectants, and why? if the hypothesis is supported, what specific experimental results will be observed? what would be the independent and dependent variable? zone size, disinfectant type, colony countJessie is working with a 100% stock concentration of Clorox. She decides to add 100 μL of bacteria to atube containing 3 mL of nutrient broth and then adds 400μL of the Clorox stock to the tube as well. Whatis the unknown concentration of the tube?The arrowhead shaped zone shows what reaction? How do you set up the test for this reaction? For which organism is this an important test?