What is the function of gRNA in CRISPR-Cas technologies?It cuts DNA at a targeted sequence and inserts a new sequence -.It cuts protein at a targeted sequence and inserts a new sequence.It ensures the Cas9 enzyme cuts at the correct place in the DNA.It ensures the Cas9 enzyme cuts at the correct place in the RNA.It replaces uracil nucleotides with thymine nucleotides.

What is the function of gRNA in CRISPR-Cas technologies?It cuts DNA at a targeted sequence and inserts a new sequence -.It cuts protein at a targeted sequence and inserts a new sequence.It ensures the Cas9 enzyme cuts at the correct place in the DNA.It ensures the Cas9 enzyme cuts at the correct place in the RNA.It replaces uracil nucleotides with thymine nucleotides.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 7QP: Cloning Genes Is a Multistep Process The following DNA sequence contains a six-base sequence that is...

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What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.
CRISPR/Cas9 can be used in genome editing. Among the following statements, which one is correct? The CRISPR and the Cas9 parts both recognise the DNA target sequence and then recruit an endonuclease for cutting it The Cas9 part recognises the DNA target sequence and the CRISPR part cuts it The CRISPR and the Cas9 parts both recognise and cut the DNA target sequence. The CRISPR part recognises the DNA target sequence and Cas9 cuts it.
Which of the following statements is not true about the guide RNA (see this interactive demonstration for help with this question: https://www.biointeractive.org/classroom-resources/crispr-cas9-mechanism-applications) Group of answer choices it contains a sequence of 20 nucleotides that matches a specific sequence in a cell’s DNA When the guide RNA is combined with Cas9, it will guide Cas9 to the target sequence its target sequence can be almost any sequence as long as it occurs near a PAM motif it is a nuclease, a type of enzyme that cleaves DNA
Which of the following is a correct statement about CRISPR-Cas-9 gene editing? Group of answer choices A single guide RNA (sgRNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence A single guide RNA (sgRNA) recognizes a genomic region followed by a long sequence palindrome repeat A single guide DNA (sgDNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence PAM sequences induce single stranded breaks that are then repaired by the CAS-9 enzyme
What is the guide RNA for? It is an RNA sequence that binds to a DNA 5'-NGG sequence where N can be any nucleotide (A, T, C, or G). It is the RNA code upon which the DNA to be inserted is built It connects the DNA sequence to the Cas9 protein It binds to the target DNA sequence, defining where Cas9 should cut the DNA It is inserted into the target DNA sequence, causing the desired gene edit
Why is genome editing by CRISPR-Cas advantageous over traditionalmethods for creating knockout or transgenic animals?Explain your answers.
Which of the following statements about the CRISPR-Cas9 technique is correct? 1. Catalytically inactive Cas-9 (dCas9) cuts only one strand of DNA. 2. The guide RNA used in the lab is analogous to the tracrRNA/crRNA hybrid in the adaptive immune system of bacteria. 3. Homology directed repair has a high degree of efficiency (ie, most target regions show integration of donor DNA). 4. NHEJ can lead to mutations. O A. 1, 2 and 3 B. 1 and 3 C. 2 and 4 D. 4 only E. All of 1, 2, 3 and 4 are correct O
What is the role of streptomycin in CRISPR experiment? What biochemical changes (DNA, protein) occurred in those cells in which CRISPR worked?
Consider the reagents required to perform CRISPR genome editing. Which molecule specifies the a new sequence that will occur at the target site if genome editing is successful? Cas9 nuclease Cas1 Cas2 tracrRNA crRNA sgRNA donor DNA
A number of advances have been made in biotechnology. CRISPR/Cas9 one of the most controversial, and has had a lot of media attention in recent years. It is a method by which scientists can precisely edit DNA sequences at exact locations. Benefits obviously include the potential to “repair” mutated genes that cause disease. In fact, preliminary results from one of the earliest clinical trials of CRISPR/Cas9 provide evidence that the technique is safe and feasible to use for treating human diseases. What other potential applications of this application do you see (you can use any organisms to illustrate your answer)? What are the potential dangers or downsides of using this technology? Do you think this technology should be used in gene editing in humans? Explain your stance.
What can serve as footprints to identify genome sites that are or have been occupied by transposable elements? options: SNPs Microsatellite DNA Transposase Transposable DNA elements at the insertion site
Which of the following are required to set up a CRISPR-Cas9 experiment to fix a mutation in a gene so that the gene sequence will be changed to the wild-type sequence? Circle all that apply. O CRISPR-Cas9 nuclease O sgRNA (single guide RNA) complementary to the part of the target sequence to be fixed O a PAM site (immediately adjacent to the target sequence in the genome) O donor DNA containing wild-type sequence of the part of the gene that is targeted (donor DNA can also be called repair template)