Q: Describe the techniques used to identify macromolecules.
A: Macromolecules are polymers that are composed of the sequence of smaller molecules called monomers.…
Q: Which of the following techniques takes advantage of NET CHARGE to purify/characterize proteins? O…
A: Proteins are purified from contaminants using a separation technique that finally produces a useful…
Q: Give applications of fluorimetric analysis with respect to A) determination of metal ions by the…
A: Fluorometric analysis is a method of chemical analysis in which a sample when exposed to radiation…
Q: A biochemist discovers and purifies a new enzyme, generating the purification table below. Procedure…
A: Specific activity of an enzyme can be calculated by dividing the number of unit by its concentration…
Q: tion chromatography separates chemicals base on their differences in
A: Chromatography is a technique that is used for the separation of various mixtures. Depending upon…
Q: Draw a structure for the compound, C3H5Br, that fits the following 1H NMR data: δ 2.32 (3H, singlet)…
A: Nuclear magnetic resonance Spectrum represents the different interactions of stereochemically…
Q: the stationaryphase is a thinlayer of silicagel or aluminaon a glass,metal or plasticplate?
A: The item to be separated is selectively adsorbed on the stationary phase of a chromatography system,…
Q: Are there any components found between the macromolecule test reagents (Benedict's, Iodine, Sudan…
A:
Q: On what basis are elements divided into macroelements and trace elements?
A: Living organisms needs elements/ minerals in order to proper growth and nutrition. These are called…
Q: Why is it important to select immiscible solvents when performing liquid-liquid extraction? In…
A: Liquid-liquid extraction is a process of separating components present in solutions by their…
Q: Which of the following techniques takes advantage of NET CHARGE to purify/characterize proteins?
A: There are many techniques to purify protein, the main principles of all these techniques is to use…
Q: How to identify the proteins and its function using mass spectrometry?
A: Mass spectroscopy is an analytical method that is used to predict the molecules present in a sample…
Q: Isoelectric focusing is used to separate …………….. based on their ................ A. salts,…
A: Electrophoresis is a method to separate the macromolecules based on their sizes under the influence…
Q: 1. B,010H22 (aq) 2. HB,010H4 (aq) 3. H2B4010H4(aq) Using the above chemicals to match the below…
A: Asked : Chemicals to be matched with given terms
Q: Explain the role of scrubbing liquid, salt, meat tenderizer, and alcohol in the experiment of…
A: Nucleic acid extraction is the method to isolate the nucleic acid from the cell. It involves three…
Q: Could Hershey and Chase have used a radioactive isotope of carbon instead of 32P? Why or why not?
A: No. They could not have used radioactive isotope of 32P because both DNA and proteins contain carbon…
Q: What are the two principal types of mass spectrometry?
A: Mass spectrometry is used to determine the mass-to-charge ratio of ions and separate them according…
Q: Comment on the boiling technique for nucleic acid extraction? Why is it done?
A: DNA extraction involves three main steps. They are, Cell disruption. DNA extraction. DNA…
Q: What is a method of separation will you use to acquire elemental iodine from potassium iodide?
A: Potassium iodide(KI) is most commonly used iodine supplement for humans and animals and is also…
Q: What is radioactivity? What does it mean for an atom to beradioactive?
A: Atoms are the smallest particle of material. Atoms are composed of subatomic particles such as…
Q: Explain how the type of decay, the half life and the chemical properties make the following…
A: Asked : Type of decay, the half life and the chemical properties for the given application.
Q: How is mass spectrometry used to identify proteins in a cell?
A: Mass spectrometry is the important method, which is used in mass determination of proteins and their…
Q: Describe the different types of chromatography used to purify proteins.
A: Introduction: Protein purification is a collection of procedures for isolating one or a few proteins…
Q: Compare and contrast the following protein characterization techniques in terms of the principles…
A: Mass spectrometry: a. It generates a beam of positively charged ions from the sample under…
Q: 430 rads of αα-particle radiation is equivalent to how many rads of X-rays in terms of biological…
A:
Q: Explain the use of mass spectrometry (MS) ?
A: Mass spectroscopy is a laboratory analytical tool. It is used to detect the mass-to-charge ratio of…
Q: The following are the absorption range for the functional group in IR spectrocopy below: D) alkyne…
A: Infrared (IR) spectroscopy is a technique that relies on the absorption of infrared radiations by…
Q: What are the benefits and disadvantages of IMAC for purifying proteins?
A: The purification of the proteins is performed by the use of different chromatographic procedures…
Q: A biochemist discovers and purifies a new enzyme, generating the purification table below. Procedure…
A: Enzymes are biological protein molecules that accelerate chemical reactions. Enzymes can be purified…
Q: What is meant by the term plating efficiency?
A: The cells are cultured over the plate at the particular growth conditions within a particular time…
Q: What is the importance and relation of biochemistry in pharmacy?
A: Biochemistry is the field of advanced science where it deals with the study of chemical reactions…
Q: Why does PET use different isotopes than that of nuclear medicine exams with gamma cameras? What…
A: Nuclear medicine is a branch of medicine that deals with the use of radioactive substances in…
Q: Why annealing, denaturation temperatures are different? Please answer at your own words.
A: Question: Why annealing, denaturation temperatures are different?
Q: Hershey & Chase labeled protein with radioactive and DNA with radioactive O phosphorus; phosphorus O…
A: Hersey and chase gave an outbreak that DNA was the genetic material.
Q: How is a forensic chemist similar to and different from a forensic toxicologist?
A: Forensic relates to law and involves scientific tests and methods in crime investigation.
Q: Define about "Mass Spectrometry for Protein Identification" ?
A: A proteomics study identifies all proteins in a biological system (e.g., cells, tissues, organisms)…
Q: What are Organic Compounds? What are Functional Groups and why are they so important in regard to…
A: What are organic compounds? Componds which contains carbon chain are called as organic componds.…
Q: One of the following is a physical denaturation of proteins except: X-ray irradiation Vigorous…
A: Protein denaturation is a wide concept and dependent on multiple factors. whenever the protein is…
Q: Which of these factors influences the radioactive decay measured by radiometric dating?…
A: Geologists use radiometric dating to estimate how long ago rocks formed and to infer the ages of…
Q: The following are denaturing agents for proteins, except:* Please choose one correct answer only.…
A: Proteins are composed of twenty standard amino acids that are attached together via peptide bonds.…
Q: Explain. What is the importance of purifying proteins?
A: Protein purification is the process by which the desired protein is isolated from a sample mixture.…
Q: What type of nuclear reaction does a CAT scan use? a. Gamma Emission b. Electron Capture c. Beta…
A: CAT scan is also known as CT (computer tomography) scan is the process of viewing the cross-section…
Q: What is the difference between the fields of Biological Chemistry and Biophysical Chemistry? In…
A: The study of chemical processes within and pertaining to live beings is known as biochemistry or…
Q: what are the other treatments on developing or improving a catalyst aside from like x-ray…
A: Catalysts can be any substance that accelerates the reaction rate without itself being consumed.…
What is “Autoradiography”? This method enables the determination of which macromolecule labeled with which isotope and how.
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- Various methods have been developed for detecting proteins. Describe how radioisotopes and autoradiography can be used for labeling and detecting proteins. How does Western blotting detect proteins?Describe the different types of chromatography used to purify proteins.Describe various detection methods used in High-Performance Liquid Chromatography (HPLC) and types of biomolecules that are detected in each method?
- Discuss the principle behind Salkowski test, reaction with ammonium ferrothiocyanate, and saponification to differentiate TAGs, phospholipid, and cholesterol. What are the general reactions involved? Include chemical reactions if necessary.To analyze a sample by mass spectrometry, the sample must consist of gas phase ions. a) Explain why it is difficult to make gas phase ions of proteins. b) Generally explain how electrospray ionization turns proteins in solution into gas phase ions.What are other chromatographic techniques that can be used for separating non-polar biomolecules such as lipids. What are the advantages (mention at least three) of using TLC over the other techniques?
- What is doping? Explain the mechanism of mass spectrometry.Draw the chemical reaction involved in each qualitative reactions of proteins. Ninhydrin test (ninhydrin solution) Xanthoproteic test (concentrated nitric acid) Millon’s test (Millon's reagent) Hopkins-Cole test (glyoxylic acid) Biuret test (Biuret solution)Explain the chemical basis (reaction) of Millon’s test. (Generic reaction and explanation) What is the difference between Millon's test and Biuret test?(minimum 5)