Using the data below from a batch test, estimate the observed yield and the decay rate of an organism growing on toluene as a substrate. Show / explain your work. Be sure to include units. Time (hrs) Toluene (mg/L) Biomass (mg VSS/L) 300 100 200 150 4 100 200 8. 25 250 16 252 24 0.1 248 36 0.1 230 48 0.1 220
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- RESULTS Please give the time-absorbance values tab? Draw graph-growth curve in Excel? Find the equation of slope line? Calculate the generation time? Choose the values in log phase Abs 0.005 25 0.003 50 0.023 75 0.026 100 0.029 125 0.035 150 0.1 175 0.26 200 0.32 225 0.375 250 0.42 275 0.46 300 0.475 325 0.489 350 0.506 375 0.515 400 0.521 425 0.526 450 0.532 475 0.522 500 0.515 525 0.514 550 0.506 575 0.489 600 0.465 625 0.42 650 0.38 675 0.36 700 0,345 725 0.333 BACTERIAL GRO WTH CURVE1.1 Microbes in the soil could be beneficial to plants and one way to evaluate this is by determining the R/S ratio. Define the R/S ratio. 1.2 Both rhizospheric and root-free soils were collected from five (5) plants before (BT) and after (AT) treatment. Using the microbial biomass that appear in Table 1, the R/S ratios before (BT) and after (AT) can be determined. Use these values to determine the respective R/S ratios. Kindly show all workings. Microbial population Before Treatment (BT) Plants R/S Microbial population After Treatment (AT) R/S ratio ratio (BT) (AT) Rhizospheric Non- soil (CFU) Rhizospheric Non- rhizospheric soil (CFU) soil (CFU) rhizospheric soil (CFU) Typha sp. 764 168 988 186 Pistia sp. 286 86 386 112 Salvinia 397 132 528 166 sp. Lemna sp. 274 121 406 152 Eichhornia 443 140 648 196 sp.Formulate a compost mixture of at consisting of least three ingredients using the Cornell Composting Spreadsheet that will satisfy the range of percent moisture content from 50-60% and the range of C:N ratio from 25 to 35. A composting bioreactor has a total capacity of 500 kg. The total weight of the mixture ranges from 400-500 kg. Example: Ingredient % Moisture % Carbon % Nitrogen Mass (Ibs.) Laying hens manure 69.0 48.0 8.0 100 Leaves, Loose and dry 15.0 48.6 0.9 65 Corn cobs 15.0 58.8 0.6 150 Water 100.0 0.0 0.0 150 Total 465 Calculated mixture moisture content: 54.0 Calculated mixture C/N ratio: 31.2
- A batch reactor was initially filled with organic substrate (S0) with an equivalentconcentration of COD = 1000 mg/L and 100 mg/L of bacterial biomass (X0). After 24 hraeration, the biomass in the reactor increased to 300 mg/L, while the substrateconcentration decreased to 200 mg/L as of COD; what is the observed biomass yield (Y)during this biodegradation process?Yeast cells has been cultured on glucose (Table 1). The growth data follows the Monod Equation Table 1: Growth data for yeast cells Glucose concentration (mg/L) 7 10 15 40 200 1000 Specific growth rate (h*¹) 0.066 0.088 0.12 0.294 0.330 0.364 0.419 0.451 0.2 0.304 0.340 0.458 Observed yield, Y'x/s (g/g) a) Calculate the maximum specific growth rate (max) and the substrate constant (Ks) b) Estimate the true yield Y'x/s and the maintainance coefficient ms c) Calculate the doubling time at a concentration of 15 mg/L glucose d) Calculate the substrate consumption rate (rs) assuming a substrate concentration of 40 mg/L and a biomass concentration of 100 mg/LTwo microorganisms that use naphthalene as a food source have the same Umax but different Ks values for naphthalene. If both types of microorganisms are inoculated at the same cell density into a liquid culture containing naphthalene as the sole carbon source (i.e. the only food source), which microorganism will outcompete the other: the one with the higher Ks or the one with the lower Ks. Why?.
- You are asked to determine the settleability of biomass in a suspended growth activated sludge sample that was brought to the laboratory. Describe the protocol you follow to achieve the objective. (If you prefer it, you can use your own numerical example to illustrate the process). Use drawings if you like to illustrate your point(s).A surface water treatment plant operates round the clock with a flaw rate of 35 m3/min. The water temperature is 15°C and Jar testing indicated and alum dosage of 25 mg/l with flocculation at a Gt value of 4 x 104 producing optimal results. The alum quantity required for 30 days (in kg) of operation of the plant isYou have received the following measurement data from microbial culture: Time (h) Biomass (g/L) 0 1.8 1.35 1.845 2.7 1.89 4.5 1.98 6.75 2.88 9 4.23 13.5 9 18 18.9 22.5 39.78 25.2 62.1 27 84.6 28.8 93.6 31.5 101.7 33.3 102.6 Calculate the specific growth rate using the start and end points of exponential growth. Give the result (1/h) to three decimal places.
- When I am doing a Generation time graph with Log CFU/mL vs Time I am getting a straight line instead of the growth curve with the phases. Is this okay or how can I get the curve?Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium whose components and concentrations are given below. Yeast extract 5 g/L Peptone 10 g/L NaCl 10 g/L Agar 1.5 g/LYour provide with 100kg of maize grain as a sample From market analysis of different parameters, Explain what will be estimated laboratory sample