Treatment with Ellman's reagent (DTNB; (5,5')-difthig-bis-(2-nitrobenzoic acid; MW =296.4 g/mol) produced an absorbance of A412 nm = 1.025. If the extinction coefficientfor the thiolate form of Ellman's reagent = 14,100 M-1-cm-1 (which arises when Ellman'sreagent reacts with a single Cys-SH group), then how many free cysteine residues(Cys-SH) are in this protein?SulfhydrylMoleculeDTNB2-R-5"OR-S-N*HS-Mixed DisulfideTNB2-

Spectroscopy is a field of analytical chemistry used to determine the amount of an unknown substance…

Answered: Treatment with Ellman's reagent (DTNB;…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Assuming the molar extinction coefficient for para-nitrophenyl phosphate (PNP) is 55,290 mol "dm cm, calculate the concentration of the molecule that produced an absorbance of 0.770 OD units in a 1.5 cm cuvette. Show all working -1 3 -1
Quinine ( C20 H24 N2 O2) is the most important alkaloid derived from cinchona bark. It is used as an antimalarial drug. For quinine, pK, = 5.1 and pK, = 9.7 ( pKp = – log Kp). Only 1 g quinine will dissolve in 1920.0 mL of solution. Calculate the pH of a saturated aqueous solution of quinine. Consider only the reaction | Q+ H2O= QH+ + OH- described by pK, where Q = quinine. pH =
80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pl. The solution is then titrated with 0.2M HCI to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCl needed to reach each relevant pKa value and equivalence point(s). Relevant pka values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.
80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pI. The solution is then titrated with 0.2M HCL to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCL needed to reach relevant pKa value and equivalence point(s)z Relevant pKa values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.
The-carotene molecule has λmax 450 nm and ɛ = 15.000 m2 / mol. Calculate the expected absorbance value for a solution in which 0.1 mg is dissolved in 10 mL of water. ( β-carotene, C40H56, 536 g/mol path length 1 cm)
MD ordered morphine SO4 gr 2/5 and the pharmacist has a stock solution of gr 1/8 per milliliter of morphine SO4. How many milliliters of the stock solution is required to fill the Rx?
What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4. Please indicate the full solutions.
The main constituents in vinegar are water and ethanoic acid (CH3COOH). In order to determine the concentration of acid in homemade vinegar, a student titrated 25 cm3 of 001 M NaOH against the vinegar. The equation for the reaction is: CH3COOH(aq) + NaOH(aq) ® CH3COONa(aq) + H2O(l) The following titration results were obtained: Burette readings (cm3) Rough 1 2 Final burette reading 20.10 38.90 31.40 Initial burette reading 0.10 20.00 12.50 Volume of vinegar used 20.00 18.90 18.90 (a) What volume of vinegar should be used in the calculation? (b) What is the mole ratio of NaOH:CH3COOH? (c) Calculate the number of moles of alkali in 25 cm3 of NaOH solution used. (d) How many moles of acid were used in the titration? (e) Calculate the…
6 x 10⁻⁵¹ = (0.15 M)² [S²⁻] [S²⁻] = (6 x 10⁻⁵¹) / (0.15 M)² Calculating, we find the minimum concentration of sulfide ion required: [S²⁻] ≈ 2.7 x 10⁻⁴⁹ M. check it calcution is write
A 10.00-mL aliquot of unknown oxalic acid solution required 15.44 mL of 0.011 17 M KMnO4 solution to reach the purple end point. A blank titration of 10 mL of similar solution containing no oxalic acid required 0.04 mL to exhibit detectable color. Find the concentration of oxalic acid in the unknown.
How many moles of EDTA (C10H16N2O3) do you have in 785µl of a 74MM EDTA solution (Mr(C10H16N203) = 292.24)? Consider all digits of your Givens significant. Round results to the appropriate number of Significant Digits. The number of moles is mmol.
Concanavalin (ConA) is a 25.5KDa protein with pl of 4.7 and optical absorbance (A 0.1% 289) of 1.14. calculate the concentration of an aqueous solution of pure BSA protein with an absorbance of 0.66 at 280nm. give your answer in units of mg/ml ?

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