Tissue culture media is a buffered nutrient solution used in laboratories to grow mammalian tissue culture cells for experiments. When cell biologists grow mammalian tissue culture cells a major issue is media acidification caused by the cells excreting metabolic byproducts. Media makers must be careful not to include too much solute in their media, as it can kill the cells through osmotic pressure imbalances, so getting the balance right is important. Based on this, if you were designing tissue culture media what ratio of acid to base would you think is best for the buffer component of your media? O90% acid: 10% base 25% acid: 75% base 75% acid: 25% base base 10% acid: 90% base O 50% acid: 50%

Tissue culture media is a buffered nutrient solution used in laboratories to grow mammalian tissue culture cells for experiments. When cell biologists grow mammalian tissue culture cells a major issue is media acidification caused by the cells excreting metabolic byproducts. Media makers must be careful not to include too much solute in their media, as it can kill the cells through osmotic pressure imbalances, so getting the balance right is important. Based on this, if you were designing tissue culture media what ratio of acid to base would you think is best for the buffer component of your media? O90% acid: 10% base 25% acid: 75% base 75% acid: 25% base base 10% acid: 90% base O 50% acid: 50%

Human Physiology: From Cells to Systems (MindTap Course List)

9th Edition

ISBN:9781285866932

Author:Lauralee Sherwood

Publisher:Lauralee Sherwood

Chapter3: The Plasma Membrane And Membrane Potential

Section: Chapter Questions

Problem 6RE

See similar textbooks

Similar questions

Gel-filtration chromatography separates molecules according to their size . Smaller molecules diffuse faster in solution than larger ones, yet smaller molecules migrate more slowly through a gel- filtration column than larger ones. explain this paradox. What should happen at very rapid flow rates?
In the pharmaceutical industry, any batch that deviates from the set parameters is designated as an ’atypical‘ batch and must be investigated before the product can be released. You are a pharmaceutical process engineer, responsible for granulating a pharmaceutical product in a 600litre mixer containing 150kg of dry powders. It was noticed while manufacturing a new batch that the liquid delivery stage ended earlier than usual and the batch contained larger granules than normal. During normal production, the impeller speed is set to 90 rpm and water is added at a flow rate of 2 litre/m n through a nozzle producing an average drop size of 400 um. Due to an incorrect setting, the actual flow rate used in the atypical batch was 3.5 litre/min and the actual drop size was estimated at 250 um. The spray width and powder surface velocity were unaffected and remained constant at 40 cm and 60 cm/s, respectively. Calculate the dimensionless spray flux for the normal case and the atypical batch,…
A dialysis bag contains 10% starch solution. The dialysis bag is placed in a beaker filled with deionized water. Ten drops of Starch indicator (iodine solution, KI) solution is placed into the deionized water. The water turns a yellowish-brown color, however, you notice that the starch solution begins to turn black. Describe what is happening with respect to the diffusion of starch.
Animal cell cultures usually require 5% carbon dioxide in the incubator. This gas along with sodium bicarbonate found in the culture medium helps to regulate what property of the cell medium? (i.e. what environmental condition does carbon dioxide/sodium bicarbonate regulate?)
Consider the solutions shown in the containers below. The composition of each solution is given in the image. 0.15 Mlactose isotonic hypotonic hypertonic crenation hemolysis no change A 0.28 M Urea B Fill the blank with the appropriate term from the list below. 0.16 MKCI The tonicity of the solution in container C is
a. Define diffusion. Is diffusion a passive or an active process? Explain how solute moves in this way. b. Use your knowledge of diffusion to explain what happened over time when you observed a crystal of methylene blue dropped into a beaker of water. Be sure to use equilibrium in your explanation. c. Explain your observations over time after a drop of methylene blue and a drop of potassium permanganate were placed in the agar. What factors affect the rate of diffusion? d. What are tissues? Name the four major types of tissues in the human body. e. Name the three primary germ layers. Name the primary germ layers from which epithelial tissues and connective tissues develop. f. List general characteristics that all epithelial tissues have in common. g. Name the functions of epithelial tissue. Which type of epithelial tissue is found in areas that need protection? h. Know how to identify simple squamous epithelium, simple cuboidal epithelium, simple columnar…
Regarding ISOTONIC solutions, which of the following are TRUE? SELECT ALL THAT APPLY A. Water movement between the ICF and EFC is equal B. Solvent concentration inside the cell and outside the cell are equal. C. Solute concentration inside the cell and outside the cell are equal. D. Solute concentraion inside the cell is equal to solvent concentration outside the cell.
In an investigation of osmosis, apple cores were submerged in different molarity solutions to determine the unknown osmolarity of the apple. To determine the molarity five uniform apple cores were massed before submerging them in different molarity solutions. After 24 hours the apple cores were massed again. Then the percent change in mass was determined for the five uniform apple cores in each molarity solution. c) Describe the environmental conditions taking place in each of the six solutions with the apples
Two to three drops of mouse blood samples were placed in three different vials containing 0.07 M NaCl,0.15 M NaCl, and 0.30 M NaCl. A drop from each of the three vials were obtained and put on a slide forobservation under HPO. The effects of the different osmotic concentrations on the cells are shown in figures in your worksheets. Label the cell membrane for each figure. Give a short description (size and cell shape) for each of the RBC samples on the space provided in your worksheet. Compare their appearances with RBCs in the blood smear. Use the following guide questions in providing descriptions for each item. Which preparation has cells that look similar as those in the blood smear? What does this indicate about the movement of water in the cells? In which solution do the cells appear differently from the normal RBCs? What part of the cell could have possibly controlled such movement of water? What is its property that allowed this movement?
Identify (state whether each item is an example of diffusion, osmosis, filtration, or active transport) 1. Movement of water from an area of lower impermeant solute concentration to an area of higher impermeant solute concentration. 2. Dye particles spread evenly through water. 3. Starch particles pass through a paper membrane. 4. A cell uses energy to "pump" sugar molecules from its external environment. 5. Water in cell moves out into a concentrated salt solution bathing the cell.
determine if osmosis or simple diffusion A crystal of blue copper sulfate is placed in a test tube of water. The next day, the solid is gone, but the water is evenly colored. A fresh potato is peeled, weighed, and soaked in a strong salt solution. The next day, it is discovered that the potato has lost a significant amount of mass. A perfume bottle is opened, and soon the odor can be sensed in all parts of the room. Garden grass wilts after being exposed to dry chemical fertilizer. Plant seeds soaked in water swell and become several times as large as before soaking. A sugar cube is dropped into a cup of hot water, and without being stirred, all of the liquid becomes sweet tasting.
Various chemical methods can be used to permeabilize or lyse cells including the use of enzymes. List three important factors to consider when developing a lysis method using enzymes. Estimate the osmotic pressure drop across the membrane of an animal cell in a 0.10 M NaCl solution, assuming that the internal total solute concentration is 0.36 osM and temperature is 30.0°C. Do you think that this pressure drop would lyse cells? Why or why not?