The term "gene expression" refers to the O process by which genetic information is transformed nucleic acid information into a functional protein. O the fact that each individual of a species has a unique set of genes. O the fact that individuals of the same species have different phenotypes. flow of information from parent to offspring.
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- What is the Shine-Dalgarno sequence? Question 15 options: A consensus sequence in prokaryotic promoters A consensus sequence in eukaryotic promoters A consensus sequence in eukaryotic enhancers A consensus sequence in prokaryotic mRNA that binds to rRNA A consensus sequence in eukaryotic mRNA that binds to rRNAWhich of the following sequences best describes the correct (5' – 3') organization of a typical eukaryotic gene? Question 12 options: TSS - TATA box – enhancer - coding sequence – BREd - termination site TATA box – TSS – BREd - coding sequence – termination site – enhancer TATA box – BREd - TSS – operon – termination site – enhancer TATA box – BREd – TSS – coding sequence – termination site - enhancer Enhancer - BREd – TSS – TATA box – coding sequence – termination siteWhich area (A, B. C. or D) represents the region important for maintaining the gene of interest during binary fission? Arabinose operon Origin region ori Ndel arac Promoter Paan PGLO (5,400bp) Ndel Ampicillin Resistance Gene Amp Hindll Ndel Green Fluorescent Protein (GFP) Gene Hindll ECORI GraphicOE Schmid/2003 D O A OB OC OD
- The symbols a, b, and c in the table below represent theE. coli lac system genes for the repressor (I ), the operator(O) region, and the β-galactosidase (Z), although notnecessarily in that order. Furthermore, the order inwhich the symbols are written in the genotypes is notnecessarily the actual sequence in the lac operon.Activity (+) or inactivity (−) of Z geneGenotype Inducer absent Inducer presenta- b+ c+ + +a+ b+ c- + +a+ b- c- - a+ b- c+/a- b+ c- + +a+ b+ c+/a- b- c- - +a+ b+ c-/a- b- c+ - +a- b+ c+/a+ b- c- + +a. Which symbol (a, b, or c) represents each of the lacgenes I, O, and Z ?b. In the table, a superscript minus sign on a gene symbolmerely indicates a mutant, but some mutant behaviorsin this system are given special mutant designations.Using the conventional gene symbols for the lac operon,designate each genotype in the table.Six strains of E. coli (mutants 1–6) that had one of thefollowing mutations (i–vi) affecting the lac operonwere isolated.i. deletion of lacYii. ocmutationiii. missense mutation in lacZiv. inversion of the lac operon (but not an inversion ofthe lacI gene)v. superrepressor mutationvi. inversion of lacZ, Y, and A but not lacI, P, oa. Which of these mutations would prevent the strainfrom utilizing lactose?b. The entire lac operon (including the lacI gene andits promoter) from each of the six E. coli strainswas cloned into a plasmid vector containing an ampicillin resistance gene. Each recombinant plasmidwas transformed into each of the six strains to create partial diploids. In analysis of these strains, mutant 1 was found to carry a deletion of lacY, so thisstrain corresponds to mutation i in the list above.Which of the other types of mutations would be expected to complement mutant 1 in these partial diploids so as to allow lactose utilization?c. In part (b), each strain was plated on…Examine the following theoretical operon (gen) in E. coli which contains structural genes (genC, genB, genA) that are involved in amino acid biosynthesis. The gen operon also includes the gene encoding for the regulator molecule within it. When the amino acid that this operon helps synthesize is present, it binds to the regulator forming a complex which can bind to the operator region. Assume that the regulator molecule only binds to the operator in the presence of the amino acid. Promoter || Operator genC genB gen operon Regulator genA
- Question 3 In constitutive mutants of the lactose operon the lactose operon is never transcribed the lactose operon is always transcribed if CRP is bound the lactose operon is transcribed in the presence of lactose none of the above L, A Moving to another question will save this response. MacBook ProOrca is a new type of E. Coli bacterium. Suppa is a nutrient that Orca needs to survive. Suppa can be synthesized by four (4) enzymes (SupA, SupB, SubC, and SupD) in its cell or it can acquire it from its environment. It only synthesizes it's own Suppa when it's really desperate, because there's none around! Draw and describe Orca's operon controlling its synthesis of Suppa.You are studying an operon containing three genesthat are cotranscribed in the order hupF, hupH, andhupG. Diagram the mRNA for this operon, showingthe location of the 5′ and 3′ ends, all open readingframes, translational start sites, stop codons, transcription termination signals, and any regions thatmight be in the mRNA but do not serve any of thesefunctions.
- How does the sigma factor of E. coli RNA polymerase bind to promoters?Question 10 options: a region of the protein binds to a stem-loop structure in the DNA a region of the protein binds to a stem-loop structure in the RNA a region of the protein inserts into the minor groove of the DNA a region of the protein binds to the negatively charged backbone of the DNA a region of the protein inserts into the major groove of the DNAPart A Which of the following could be used to turn on a regulon (composed of multiple genes and operons) in a bacterium? There are multiple correct answers. You must select all correct options1 O use riboswitches that all respond to the same molecule O activate an alternative RNA polymerase mutate (change) a promoter sequence V activate an alternative sigma factor O specifically degrade one kind of repressor protein Submit Previous Answers Request Answer X Incorrect; Try Again; 3 attempts remainingExamine the following theoretical operon (gen) in E. coli which contains structural genes (genC, genB, genA) that are involved in amino acid biosynthesis. The gen operon also includes the gene encoding for the regulator molecule within it. When the amino acid that this operon helps synthesize is present, it binds to the regulator forming a complex which can bind to the operator region. Assume that the regulator molecule only binds to the operator in the presence of the amino acid. Promoter || Operator genC genB gen operon Regulator genA