The relative displacement (r/ro) of bovine serum albumin was observed as a function of time: t (s) 700 3580 4540 5020 r/r. 1.0129 1.0679 1.0871 1.0965 Given w = 6 260 /s, find the sedimentation coefficient. Assuming v = 7.34x 10 3 m3 kg and p = 9.93 x 102 kg/m3, diffusivity = 6.97 x 10-11 m2/s at 25 °C, determine the molar %3D %3D mass of the sample in kg/mol.
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- You prepared a stock solution by dissolving a compound with the molar mass of 110.65 in a 100 mL volumetric flask. Then you removed a 2 mL aliquot and placed it in a 250 mL volumetric flask and dilute it to the mark. The absorbance of this diluted solution in the 250 mL volumetric flask at 288 nvm was 0.421 using a 2.50 cm path length curvet. The molar absorptivity for this compound at 288 nvm is 65000 M-1cm-1 How many ng of the compound were used to make the stock solution? a)1.44 b)0.96 c)0.72 d)1.22 e)2.87A 10 cm 3 air saturated Fricke dosimetry solution in a 1 cm diameter tube is irradiated for 10 min in a 6~ source of gamma rays. The optical density measured at 304 txm in a 1 cm light path at 30~ was 0.260 after the completion of irradiation and 0.003 before irradiation. (a) What is the total dose in gray absorbed by the solution?(b) If 10 cm 3 of methanol is irradiated in the same tube for the same 10min, what is the absorbed dose in gray. The Z/A for methanol is 0.562 and that for the Fricke dosimeter is 0.553. Explain any assumption you make to solve the problem.A solution was prepared using 1.0 mg of a compound of Molecular weight 160 g / mol isdissolved in 10 mL of Ethanol and part of the solution is placed in a 1 cm UV cell. TheUV spectrum is taken at a (I: 247 nm). The maximum absorbance is 0.5. Determine the value of molar absorptivity (c) in M ^ -1cm ^ -1
- 3.) The accuracy of a spectrophotometer is evaluated by preparing a solution of 60.06 ppm K2Cr2O7 and measuring its absorbance at a wavelength of 350 nm in a cell with a pathlength of 1.00 cm. The expected absorbance is 0.640. What is the expected molar absorptivity of K2Cr2O7 at this wavelength?(a) You measure the percent transmittance of a solution containing chromophore X at 400nm in a 1-cm path length cuvette and find it to be 50%. What is the absorbance of this solution? (b) What is the molar absorptivity of chromophore X if the concentration of X in the solution measured in question (a) is 0.5mM? (c) What is the concentration range of chromophore X that can be assayed if, when using a sample cell of path length 1, you are required to keep the aborbance between 0.2 and 0.8?The accuracy of a spectrophotometer can be evaluated by preparing a solution of 60.06-ppm K2Cr2O7 in 0.0050 M H2SO4 and measuring its absorbance at a wavelength of 350 nm using a cell with a pathlength of 1.00 cm. The absorbance should be 0.640. What is the molar absorptivity of K2Cr2O7 at this wavelength?
- A Dubosq colorimeter consists of a cell of fixed path length and a cell of variable path length. By adjusting the length of the latter until the transmission through the two cells is the same. the concentration of the second solution can be inferred from that of the former. Suppose that a plantdye of concentration 25 μg dm-3 is added to the fixed cell. the length of which is 1.55 em. Then a solution of the same dye. but of unknown concentration. is added to the second cel l. It is found that the same t ransmittance is obtained when the length of the second cel l is adjusted to 1.18 em. What is the concentration of the second solution?An atomic absorption method for the determination of the amount of iron present in used jet engine oil was found from pooling 30 triplicate analyses to have a standard deviation s = 3.1 µg Fe/mL. If s is a good estimate of o, calculate the 95 and 99% confidence intervals for the result 21.7 µg Fe/mL if it was based on the following criteria. (a) a single analysis 95% confidence interval= 21.7 + 99% confidence interval = 21.7 + (b) the mean of two analyses 95% confidence interval = 21.7 + 99% confidence interval= (c) the mean of six analyses 21.7 ± 95% confidence interval = 21.7 + 99% confidence interval= 21.7 + μg Fe/mL μg Fe/mL μg Fe/mL μg Fe/mL μg Fe/mL μg Fe/mL22-30. (a) The mean free path is the average distance a molecule travels before colliding with another molecule. The mean free path, A, is given by A = kT/(V20P), where k is Boltzmann’s constant, T is temperature (K), P is pressure (Pa), and o is the collision cross section. For a molecule with a diameter d, the col- lision cross section is nd. The collision cross section is the area swept out by the molecule within which it will strike any other molecule it encounters. The magnetic sector mass spectrometer is maintained at a pressure of ~103 Pa so that ions do not collide with (and deflect) one another as they travel through the mass analyzer. What is the mean free path of a molecule with a diam- eter of 1 nm at 300 K in the mass analyzer?
- A standard of solution was put through appropriate dilutions to give the concentrations of glucose (mM) shown in the accompanying table. The absorbances in the table (1.00-cm cells) were recorded at 490 m table. Use the method of least squares to find an equation relating absorbance and the concentration of glucose.Absorbances of a series of phosphate standard solutions measured using UV- Vis spectrophotometer using 1.0 cm pathlength glass cuvette at 415 nm are given in table 2 below. 7. Table 2 Standard solution Concentration (mmol/L) Absorbance Std 1 0.0 0.1 Std 2 0.15 Std 3 0.2 0.28 Std 4 0.3 0.4 Std 5 0.4 0.55 Std 6 0.5 0.7 If an unknown sample exhibits an absorbance of 0.45. Determine the phosphate concentration in this unknown sample solution, using: a) Calibration plot b) Least-square methodLlë 4 A (0.002M) Cu2+ solution gave an absorbance of O.50 at 560 nm in a 1.0 cm :cell. The molar absobitivity of solution is equel * 2.24 O 250 1120 O 0.004