The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67
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The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar.
I - 0.25
II - 0.84
III - 0.56
IV- 0.67
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- what is chromatography? Explain different types of chromatography and explain how to seperate compounds. indicate stationary phase mobile phase.1. How does chromatography separate the components of leave samples? 2. Can you use chromatography to purify a mixture? why? 3. What is Rf value and its significance in chromatography?Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.
- What does a refractometer reading of 2 mean when distilling ethanol?The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67 Group of answer choices II > IV > III > I I > III > IV > II I > II > III > IV IV > II > III > IColumn chromatography is also very useful for separating mixtures of organic compounds that are not coloured. Briefly explain how you could use column chromatography for the separation of a colourless mixture, and how you could identify your separated compounds.
- What kind of sample cleanup does solid-phase extraction accomplish in the analysis of caffeine and theobromine in chocolate?Briefly explain how cation exchange chromatography can used in protein separation?Chemistry Briefly describe the differences between the scanning and SIM approaches with reference to your data. Discuss how and why your scanning (TIC) and SIM (mass chromatograms look different. Pay particular attention to selectivity of compounds detected and signal-to-noise. What are some advantages of SIM? What are some disadvantages?
- A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.144. Assuming that the best fit linear line of the standard curve was y=0.04144x+0.01521 (μgmL), calculate the percent protein by mass in the original protein powder.3. What is particulate gravimetry? When do we use this technique? 4. What are the conditions for a successful gravimetric analysis. 5. Define nucleation. What conditions are important for this stage.Identify the detectors in gas chromatography that can be used for the detection of each type of analyte. More than one detector may be appropriate for a given analyte, and a given detector may be appropriate for more than one type of analyte. compounds ionized by UV radiation sulfur or nitrogen containing compounds halogenated compounds flame ionization Answer Bank mass spectrometer nitrogen and phosphorous containing compounds thermal conductivity electrolytic conductivity hydrocarbons electron capture thermionic photoionization