Sweetzyme IT Extra is a trademarked immobilized enzyme formulation of glucose isomerase sold by the Danish company Novozymes. The following reaction is carried out in a 4.0 L reactor in the presence of an non-competitive inhibitor, I: E + G <=1=> EG =2=> E + F EG+1 <=3 => EIG G = glucose, F = fructose The Sweetzyme beads added to the reactor have a total surface area, a = 342 cm². Kinetic Parameters k₁= 0.00820 M-¹ s¹ k.₁ 0.00190 s¹¹ k₂ = 0.570 s¹ Initial/Total Enzyme concentration: Initial Bulk Substrate Concentration: Mass transfer coefficient, Eo 2.30 x 105 M Sb = 0.152 M k₁= 9.15 x 10 cm/s A. What is the maximum reaction velocity with no inhibitor (in mol/L-s)? (recall that Rapid Equilibrium and Quasi steady state approaches predict the same value for Vmax for this mechanism) B. What is the maximum reaction velocity (in mol/L-s) with the non-competitive inhibitor, I, at a concentration of [1] = 0.0029 M, with K₁= 1.8 x 10³ M.

Sweetzyme IT Extra is a trademarked immobilized enzyme formulation of glucose isomerase sold by the Danish company Novozymes. The following reaction is carried out in a 4.0 L reactor in the presence of an non-competitive inhibitor, I: E + G <=1=> EG =2=> E + F EG+1 <=3 => EIG G = glucose, F = fructose The Sweetzyme beads added to the reactor have a total surface area, a = 342 cm². Kinetic Parameters k₁= 0.00820 M-¹ s¹ k.₁ 0.00190 s¹¹ k₂ = 0.570 s¹ Initial/Total Enzyme concentration: Initial Bulk Substrate Concentration: Mass transfer coefficient, Eo 2.30 x 105 M Sb = 0.152 M k₁= 9.15 x 10 cm/s A. What is the maximum reaction velocity with no inhibitor (in mol/L-s)? (recall that Rapid Equilibrium and Quasi steady state approaches predict the same value for Vmax for this mechanism) B. What is the maximum reaction velocity (in mol/L-s) with the non-competitive inhibitor, I, at a concentration of [1] = 0.0029 M, with K₁= 1.8 x 10³ M.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter18: Glycolysis

Section: Chapter Questions

Problem 18P: Distinguishing the Mechanisms of Class I and Class I Aldolases Fructose bisphosphate aldolase in...

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