Question 3. Your professor asks you to make a staining solution to stain and observe specimens under the microscope. You are told to prepare 100 mL of staining solution containing 40% methanol, 1X hematoxylin dye and 0.01 M calcium chloride (CaC12). In the histology lab, you find a stock of 100% pure methanol (liquid), a 10x hematoxylin dye stock solution (liquid), plenty of water and a stock solution of 5M CaC12. There are also all kinds of pipets, cylinders and beakers to measure volumes. a) Describe how you make the staining solution? b) On your following trip to the histology lab, you realize that all the pipets to accurately measure volumes smaller than 1 ml are broken. What alternative method do you need to use to avoid pipetting small volumes (just explain in a couple of sentences, no calculation needed)?

Principles Of Pharmacology Med Assist
6th Edition
ISBN:9781337512442
Author:RICE
Publisher:RICE
Chapter15: Antibiotic Agents
Section: Chapter Questions
Problem 16RQ
icon
Related questions
Question
Question 3.
Your professor asks you to make a staining solution to stain and observe specimens under the
microscope. You are told to prepare 100 mL of staining solution containing 40% methanol, 1X
hematoxylin dye and 0.01 M calcium chloride (CaC12).
In the histology lab, you find a stock of 100% pure methanol (liquid), a 10x hematoxylin dye
stock solution (liquid), plenty of water and a stock solution of 5M CaC12. There are also all kinds
of pipets, cylinders and beakers to measure volumes.
a) Describe how you make the staining solution?
b) On your following trip to the histology lab, you realize that all the pipets to accurately
measure volumes smaller than 1 ml are broken. What alternative method do you need to use to
avoid pipetting small volumes (just explain in a couple of sentences, no calculation needed)?
Transcribed Image Text:Question 3. Your professor asks you to make a staining solution to stain and observe specimens under the microscope. You are told to prepare 100 mL of staining solution containing 40% methanol, 1X hematoxylin dye and 0.01 M calcium chloride (CaC12). In the histology lab, you find a stock of 100% pure methanol (liquid), a 10x hematoxylin dye stock solution (liquid), plenty of water and a stock solution of 5M CaC12. There are also all kinds of pipets, cylinders and beakers to measure volumes. a) Describe how you make the staining solution? b) On your following trip to the histology lab, you realize that all the pipets to accurately measure volumes smaller than 1 ml are broken. What alternative method do you need to use to avoid pipetting small volumes (just explain in a couple of sentences, no calculation needed)?
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Forensic Analysis
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Principles Of Pharmacology Med Assist
Principles Of Pharmacology Med Assist
Biology
ISBN:
9781337512442
Author:
RICE
Publisher:
Cengage
Essentials of Pharmacology for Health Professions
Essentials of Pharmacology for Health Professions
Nursing
ISBN:
9781305441620
Author:
WOODROW
Publisher:
Cengage
Principles Of Radiographic Imaging: An Art And A …
Principles Of Radiographic Imaging: An Art And A …
Health & Nutrition
ISBN:
9781337711067
Author:
Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:
Cengage Learning