Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter2: Basic Hematology
Section2.3: Microhematocrit
Problem 3RQ
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Question:
1. Enumerate the different application of Dyna Bead Analysis.
2. Discuss the significance of this method.
Transcribed Image Text:Dynabead Applications for Genome Analysis
Applications include: Differential display, 5'RACE, S1 nuclease mapping, DNase footprinting,
purification of RNA/DNA binding proteins, and subtractive hybridization for isolation of tumor
specific alterations and identification of toxin genes in pathogens.
Streptavidin-Coupled Dynabeads or Dynabeads Oligo (dT)25 (Cat. Nos. 61002, 61005) are used
in many gene expression profiling methods, e.g. based on representational cloning and
sequencing of cDNAs from libraries.
Double stranded DNA sequences longer than 2 kb are effectively immobilized with
the Dynabeads kilobaseBINDER Kit.
Streptavidin-coupled Dynabeads magnetic beads used in a RTPCR/ECL assay provide a
practical and sensitive approach for detection of various metastatic cancers in tissues and
blood.
The beads have also been cited in numerous published articles on the SAGE® technology and
in a related method published by S. Brenner et
IV.
QUESTIONS:
1. Enumerate the different application of Dyna Bead Analysis.
2. Discuss the significance of this method.
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