Provide a simplified schematic presentation of (non-cyclic) photophosphorylation. Clearly indicate the location and direction of electron and proton flow as well as organelle compartments. (You can also draw the Z-scheme but then you have to include ADP phosphorylation).
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Provide a simplified schematic presentation of (non-cyclic) photophosphorylation. Clearly indicate the location and direction of electron and proton flow as well as organelle compartments. (You can also draw the Z-scheme but then you have to include ADP phosphorylation).
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- (c) Compare the differences between oxidative phosphorylation and photophosphorylation by redrawing (if necessary) and completing the table given below: Table 1: Comparison of oxidative phosphorylation and photophosphorylation Oxidative phosphorylation Photophosphorylation Organelle Source of electrons Final electron acceptor Source of energy Role of ATPIdentify the chemical basis for ApH and AY across the chloroplast thylakoid membrane by dragging the descriptions to their targets. Be sure to notice that the upper arrow iindicates ApH and the lower arrow indicates ΔΨ. ATP synthase complex H+ N ADP + P₁ Light energy ATP H*N Photosystem I/II- Chloroplast N side Aus PN ApH T + Thylakoid membrane HTp H+p Lumen Stroma P side Proton circuit A B High H concentration Low positive charge High positive charge Low H+ concentration Within the image, identify the types of proton translocation by dragging each label to its target. O XH₂ 2H+ + Z 2 H* ZH₂ O XH₂ Z 2H+ ZH₂ 2H+ C A B Proton pump Redox loopFor the following questions, choose one to discuss: chloroplast/photosynthesis State at the outset which one you will discuss. A) What role do proton gradients play in the process of photosynthesis proton gradients allow B) Where are proton gradients formed? Within what structures are they seen in chloroplasts How do the structures help them to maintain a gradient? C) Explain where and how the chloroplast or mitochondria uses passive transport and active transport to complete photosynthesis or cellular respiration.
- Thylakoids were isolated from chloroplasts and incubated in the dark in an acidic solution (pH 4) to equilibrate the pH. After 30 minutes, the thylakoids were transferred to a basic solution (pH 8) and kept in the dark. Will this system produce ATP? Explain. Will this system produce G3P? Explain.Describe cyclic and non cyclic photo phosphorylation. Include all the stepsDescribe the chemiosmotic coupling mechanism. Draw a simple picture illustrating this mechanism and how ATP is synthesized in mitochondria. Label the components.
- The Relative Efficiency of ATP Synthesis in Noncyclic versus Cyclic Photophosphorylation If noncyclic photosynthetic electron transport leads to the translocation of 7 H+/2e- and cyclic photosynthetic electron transport leads to the translocation of 2 H+/e-, what is the relative photosynthetic efficiency of ATP synthesis (expressed as the number of photons absorbed per ATP synthesized) for noncyclic versus cyclic photophosphorylation? (Assume that the CF1CF0-ATP synthase yields 3 ATP/14 H+.)Consider the structures and functions of mitochondria and chloroplasts. For each of the statements below, identify which part(s) of the chloroplast or mitochondrion (identified by letters in the figure below) are described. Some answers may include more than 1 letter. In those cases, separate the letters by a single space (eg. c g) Electron transport chains are located in ------ Photosystem I and II are located in ------- Ubiquinone is located in ------- NADPH is produced in ------ Pyruvate oxidation takes place in ------ ATP is produced in ------ NAD+ is produced in ----- High H+ concentration is produced in ----- O2 is produced in ----- RuBP is produced in ------Daniel Arnon and coworkers carried out experiments with intact, salt- washed chloroplasts to study photophosphorylation. When the chloroplasts were illuminated in the presence of ADP +P, ATP was produced, but oxygen was not produced or nor consumed. ATP formation was not accom- panied by a measurable electron transport involving any external electron donor or acceptor. The overall reaction for this result is: ADP + P -", ATP When NADP+ was included in addition to the ADP + P, ilumination of the intact chloroplats again resulted in resulted in the photophosphorylation of ADP to ATP. In addition, the NADPpt was reduced to NADPH + H* and o, was produced. Moreover, the light-induced reduction of NADP* was greatly decreased if ADP + P; were ommitted. The equation for this reaction is: NADP* + H,0 + ADP + R -", NADPH + H + ATP + }02 Briefly describe the mechanism(s) of these two types of photophosphoryla- tion that explain all of these results.
- Assume a pH gradient of 4.0 units across a thylakoid membrane, with the lumen more acidic than the stroma.What is the standard free energy change per mol O2 produced? How does this compare to the energy required to drive the synthesis of ATP?Calculate (in kJ/mol) the energy that is required by the Rhodopseudomonas viridis photophosphorylation system to synthesize 3 ATP? (Assume that the R. viridis F1F-ATP synthase c-subunit rotor contains 12 c-subunits and that the R. viridis cytochrome b/c complex translocates 2 H/e". Also assume that the wavelength of lig! is 680 nm.) Number of photons required to synthesize 3 ATP = photons Energy = kJ/molAssume a pH gradient of 4.0 units across a thylakoid membrane, with the lumen more acidic than the stroma. What is the longest wavelength of light that could provide enough energy per photon to pump one proton against this gradient, assuming a 20% efficiency in photosynthesis and T = 25 °C?