In an experiment, separation of albumin from chicken liver was attempted at 31%, 58%, and 65% saturation with ammonium sulfate, (NH4)2SO4. After homogenization and centrifugation, 23 ml of the supernatant was obtained. What is the volume of saturated buffered (NH4)2SO4 (pH 7.0) that must be added to make the solution 31% saturated?
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- What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4. Please indicate the full solutions.N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic A purified protein is in a Hepes acid) buffer at pH 7 with 375 mM NaCl. A dialysis membrane tube holds a 2.0 mL sample of the protein solution. The sample tube floats in a beaker containing 1.00 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na+, Cl-, and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 2.0 mL protein sample after dialysis in 150 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaCl] after a single dialysis: [NaCl] after a double dialysis: mM mMThimerosal Tincture USP contains 0.1% w/v thimerosal and 50% v/v ethyl alcohol. If the cap is left off of a 15-mL bottle of the tincture, and the ethyl alcohol evaporates leaving a final volume of 9.5 mL, what is the concentration of thimerosal in the evaporated solution expressed as a ratio strength?
- 80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pl. The solution is then titrated with 0.2M HCI to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCl needed to reach each relevant pKa value and equivalence point(s). Relevant pka values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.How much caffeine can be extracted using 50.0ml of DCM if a tea bag contains 15 grams of tea leaves with 0.9% of caffeine? And has undergone solid-liquid extraction with 100ml of distilled water followed by liquid-liquid extraction using DCM. The solubility of caffeine requires 15 ml of water and 2.0ml of DCM for 40.0mg.Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.
- Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength. Answer: You did not give the correct unit. The correct answer is: 333 mL XStreptomycin sulfate 400 mg IM daily is ordered for a child weighing 77lbs. The recommended dosage is 20-40 mg/kg/day once daily. A 1 gram vial of streptomycin sulfate is available in powdered form with the following instructions: Dilution with 1.8 mL of sterile water will yield 400mg per mL. If safe, how many mLs will you give?A 10.00-mL aliquot of unknown oxalic acid solution required 15.44 mL of 0.011 17 M KMnO4 solution to reach the purple end point. A blank titration of 10 mL of similar solution containing no oxalic acid required 0.04 mL to exhibit detectable color. Find the concentration of oxalic acid in the unknown.
- N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) buffer at pH 7 with 400 mM NaCl. A A purified protein is in a Hepes dialysis membrane tube holds a 5.0 mL sample of the protein solution. The sample tube floats in a beaker containing 0.50 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na*, CI", and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 5.0 mL protein sample after dialysis in 300 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaCl] after a single dialysis: [NaCl] after a double dialysis: mM mM80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pI. The solution is then titrated with 0.2M HCL to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCL needed to reach relevant pKa value and equivalence point(s)z Relevant pKa values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.Describe the preparation of 2.00 L of 0.100 M glycine buffer, pH 9.0, from glycine and 1.00 M NAOH. What mass of glycine is required, and what volume of 1.00 NaOH is required? The appropriate pK, of glycine is 9.6.