In a typical microbiology laboratory, reasons for no bands from a gel of a polymerase chain reaction may be due to errors relating to omission of ingredients in the reaction mix and absence of the target sequence in the template DNA. Based on (i) primer problem and (ii) purity/potential contamination of the target sequence, explain the reason for non-appereance on bands.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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In a typical microbiology laboratory, reasons for no bands from a gel of a polymerase chain reaction may be
due to errors relating to omission of ingredients in the reaction mix and absence of the target sequence in
the template DNA. Based on (i) primer problem and (ii) purity/potential contamination of the target sequence, explain the reason for non-appereance on bands.

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