If a selection assay be made to identify cells that have incorporated the recombinant DNA, what type of medium will be used? How will it work?

Q: When a company changes from the LAL assay to the Recombinant Factor C assay, they must perform a…

A: LAL or Limulus amebocyte lysate assay is a technique used to assess and quantify the presence of…

Q: What is southern blot? Understand PCR reaction in detail. What polymerase enzyme is used in PCR…

A: Both southern-blotting and PCR are types of recombinant DNA technology. Recombinant DNA technology…

Q: How does an RT-PCR work?

A: RT- PCR is a technique used in genetic studies used for the detection and quantification of mRNA.…

Q: Do you think a U-tube could be used to distinguish betweentransduction and transformation?

A: Transformation is the process of horizontal gene transfer, where the genetic material moves from one…

Q: Although many cloning applications involve introducing recombinant DNA into bacterial host cells,…

A: Cloning can be defined as the process by which identical copies of the organisms are created. More…

Q: Why is it difficult in a single experiment to transfer a largenumber of genes to a recipient cell…

A: Gene transfer is the process of insertion of unrelated DNA into cells. There are three mechanisms of…

Q: What phase of PCR (exponential, linear, or stationary) is analyzedto quantitate the amount of DNA or…

A: The genetic entity of the organism is mainly composed of nucleic acids. The nucleic acids DNA and…

Q: Describe a method of labeling a DNA probe. Explain how this probe maybe detected after DNA…

A: The probe is basically a single stranded sequence of DNA or RNA which is used commonly for the…

Q: In rRT–PCR, why do need to convert RNA to DNA first? Why can’t you amplify the RNA molecule…

A: Taq polymerase does not work on RNA samples, so PCR cannot be used to directly amplify RNA…

Q: How are probes used to screen DNA libraries? Explain how a synthetic probe can be prepared when the…

A: A probe is an oligonucleotide stretch of DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) which…

Q: Explain The replica plating technique?

A: Replica plating is used in the microbiological experiment. It helps in the testing of various…

Q: Describe and contrast the common steps of DNA replication in vivo and the PCR reaction in vitro?

A:

Q: In the bacterial transformation experiment, what is the primary purpose of using an…

A: Microbiology is the branch of science that deals with the study of microorganisms that are too small…

Q: Why is PCR beneficial?

A: PCR is a very useful tool and has many applications in various fields such as medical, forensic,…

Q: which is the delivery techniques can bbe used to best transfer recombinant plasmids into E.coli…

A: ANSWER) The process of transferring the recombinant plasmids into the E.coli cells is called…

Q: Can we do PCR on a plasmid?

A: In molecular biology, the plasmid is most abundantly used. The plasmid is a circular autonomously…

Q: In gene mapping using generalized transduction, bacterial genes that are cotransduced are a. far…

A: Transduction is a process used to map the bacterial genes. The transduction is of two types,…

Q: What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with…

A: In the conventional amplification technique of PCR (polymerase chain reaction), the amplified…

Q: Coomassie dye is the equivalent of what component used in analysis of DNA by agarose gel…

A: Western blot helps to identify specific proteins from a mixture of proteins and this step follows…

Q: By seeing the picture, how are Recombinant DNA formed? What is the difference between genetic…

A: Introduction: Genetic engineering or genetic modification or genetic manipulation involves direct…

Q: To maximize the number of mutations following UV irradiation, should you incubate the irradiated…

A: A mutation is a modification that happens in the deoxyribonucleic acid (DNA) sequence, either due to…

Q: What are the reagents used for Reverse Transcriptase PCR (RT-PCR) and Reverse Transcriptase–Real…

A: Introduction Reagents are substances used for the detection or determination of another substance,…

Q: What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with…

A: The amplification of the DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) can be performed by…

Q: What is artificial transformation used for?

A: Transformation is a process in which an organism acquires exogenous DNA. This process occurs in two…

Q: Explain what is  Flow cell DNA sequencing ?

A: What is DNA sequencing? It is the process of finding out the order of nucleotides i.e. adenine,…

Q: Describe the steps, starting from an endonuclease digested DNA sample, to complete a Southern blot…

A: Southern blotting is a hybridization method for identifying a specific DNA sequence in the host DNA.…

Q: Would it be appropriate to use DNA probes such as VNTR in DNA fingerprinting of an bacteriophage?

A: Any group of Viruses that attach to the bacteria and infect the bacteria is known as bacteriophage.…

Q: . In the PCR process, if we assume that each cycle takes5 minutes, how manyfold amplification would…

A: Introduction: PCR(Polymerase Chain rlReaction) is a technique used to amplify a single copy or a few…

Q: How is DNA amplified in a polymerase chain reaction(PCR) procedure?

A: Step 1 Polymerase chain reaction (PCR) sometimes also called molecular photocopying is a technique…

Q: In DNA cloning, why do we need to check colonies for insert after transformation?

A: Gene is the basic unit of heredity. All living organisms contain certain nucleic acids as their…

Q: How does RT-PCR differ from traditional PCR?

A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…

Q: distinguish between bacterial cells that obtained the plasmid and those that did not

A: Bacteria are single celled organisms with a unique internal structure.

Q: Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells…

A: An example of a reporter gene is the Lac Z gene, this gene encodes for fluorescent proteins in the…

Q: Describe the technique of in situ hybridization. Explain how it can be used to map genes.

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: Gel Electrophoresis Separates DNAFragments According to which thing?

A: Electrophoresis is a technique used for separating the components of a mixture of charged molecules…

Q: blue-white colony selection method in the recombinant colony screening

A: Blue - white screening for selection recombinat colony :- Blue - white screening is a rapid and…

Q: The purpose of using soap in the DNA extraction activity using frozen strawberries is to

A: A gene is an area of DNA molecule or polymer of amino acid that encodes function. A chromosome…

Q: how can PCR products be labeled using fluorescence?

A: Polymerase Chain Reaction (PCR) is a molecular biology technique that is used for various purposes…

Q: Can you explain how PCR( Polymerase Chain Reaction can detect very) low amounts of DNA?

A: Polymerase Chain Reaction (PCR) is a molecular biology technique that is used to amplify a copy of…

Q: Vhat is needed from the cells for PCR?

A: The polymerase chain reaction (PCR) is a technique for replicating a piece of DNA millions of times.…

Q: What determines the size (length) of the primary PCR product?  What might a successful gel check of…

A: PCR stands for polymerase chain reaction. It is used to amplify the specific region of gene of…

Q: What are five variables to consider when designing a primer for standard PCR and state why.

A: Polymerase chain reaction involves amplification of desired DNA strand using three steps i.e..…